Identification of human autoantibodies to transcription factor IIB

Francena D. Abendroth, Scott R. Peterson, Matthew Galman, Akira Suwa, John A. Hardin, William S. Dynan

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We have characterized the ability of various human autoimmune sera to react with RNA polymerase II transcription factors. One serum, which strongly Inhibited transcription in a cell-free system, was shown to contain antibodies directed against human TFIIB. The serum did not show reactivity against the other general transcription factors, including human TBP, TRIE and TF1IF. The inhibition of transcription was directly attributable to depletion of TFIIB activity, as demonstrated by reconstftution of activity with recombinant TFIIB. It has long been recognized that components of the RNA processing machinery are major human autoantigens. The present results show that at least one general transcription factor required for messenger RNA synthesis Is an autoantigen as well.

Original languageEnglish (US)
Pages (from-to)2770-2774
Number of pages5
JournalNucleic acids research
Volume23
Issue number14
DOIs
StatePublished - Jul 25 1995

ASJC Scopus subject areas

  • Genetics

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    Abendroth, F. D., Peterson, S. R., Galman, M., Suwa, A., Hardin, J. A., & Dynan, W. S. (1995). Identification of human autoantibodies to transcription factor IIB. Nucleic acids research, 23(14), 2770-2774. https://doi.org/10.1093/nar/23.14.2770