TY - JOUR
T1 - Identification of Helicobacter spp. in bile and gallbladder tissue of patients with symptomatic gallbladder disease
AU - Sabbaghian, M. Shirin
AU - Ranaudo, Jeffrey
AU - Zeng, Lin
AU - Alongi, Alexandra P.
AU - Perez-Perez, Guillermo
AU - Shamamian, Peter
PY - 2010/3
Y1 - 2010/3
N2 - Background: This experimental study was designed to determine if Helicobacter spp. contribute to benign gallbladder disease using polymerase chain reaction (PCR) methods. Methods: Patients with benign gallbladder disease scheduled for elective cholecystectomy at New York University Langone Medical Center were recruited from February to May 2008. Bile, gallbladder tissue and gallstones were collected. DNA was isolated from these specimens and amplified via PCR using C97F and C98R primers specific for Helicobacter spp. Appropriate positive and negative controls were used. Products were analysed with agarose gel electrophoresis, sequenced and results aligned using SEQUENCHER. Plasma was collected for detection of anti-Helicobacter pylori antibodies via enzyme-linked immunosorbent assay. Results: Of 36 patients, 12 patients' bile and/or tissue were positive for Helicobacter spp. by PCR. Species were most homologous with H. pylori, although other Helicobacter spp. were suggested. Six of 12 patients demonstrated anti-Helicobacter antibodies in plasma, suggesting that the remaining six might have demonstrated other species besides H. pylori. Four of six plasma samples with anti-Helicobacter antibodies were anti-CagA (cytotoxin associated gene) negative. Discussion: Helicobacter spp. can be detected in bile and gallbladder tissue of patients with benign gallbladder disease. The contribution of these bacteria to the pathophysiology of gallbladder disease and gallstone formation requires further study.
AB - Background: This experimental study was designed to determine if Helicobacter spp. contribute to benign gallbladder disease using polymerase chain reaction (PCR) methods. Methods: Patients with benign gallbladder disease scheduled for elective cholecystectomy at New York University Langone Medical Center were recruited from February to May 2008. Bile, gallbladder tissue and gallstones were collected. DNA was isolated from these specimens and amplified via PCR using C97F and C98R primers specific for Helicobacter spp. Appropriate positive and negative controls were used. Products were analysed with agarose gel electrophoresis, sequenced and results aligned using SEQUENCHER. Plasma was collected for detection of anti-Helicobacter pylori antibodies via enzyme-linked immunosorbent assay. Results: Of 36 patients, 12 patients' bile and/or tissue were positive for Helicobacter spp. by PCR. Species were most homologous with H. pylori, although other Helicobacter spp. were suggested. Six of 12 patients demonstrated anti-Helicobacter antibodies in plasma, suggesting that the remaining six might have demonstrated other species besides H. pylori. Four of six plasma samples with anti-Helicobacter antibodies were anti-CagA (cytotoxin associated gene) negative. Discussion: Helicobacter spp. can be detected in bile and gallbladder tissue of patients with benign gallbladder disease. The contribution of these bacteria to the pathophysiology of gallbladder disease and gallstone formation requires further study.
KW - Cholecystitis
KW - Gallbladder
KW - Gallstones
KW - Helicobacter
UR - http://www.scopus.com/inward/record.url?scp=77449114943&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77449114943&partnerID=8YFLogxK
U2 - 10.1111/j.1477-2574.2009.00148.x
DO - 10.1111/j.1477-2574.2009.00148.x
M3 - Article
C2 - 20495657
AN - SCOPUS:77449114943
SN - 1365-182X
VL - 12
SP - 129
EP - 133
JO - HPB
JF - HPB
IS - 2
ER -