Identification of Helicobacter spp. in bile and gallbladder tissue of patients with symptomatic gallbladder disease

M. Shirin Sabbaghian, Jeffrey Ranaudo, Lin Zeng, Alexandra P. Alongi, Guillermo Perez-Perez, Peter Shamamian

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: This experimental study was designed to determine if Helicobacter spp. contribute to benign gallbladder disease using polymerase chain reaction (PCR) methods. Methods: Patients with benign gallbladder disease scheduled for elective cholecystectomy at New York University Langone Medical Center were recruited from February to May 2008. Bile, gallbladder tissue and gallstones were collected. DNA was isolated from these specimens and amplified via PCR using C97F and C98R primers specific for Helicobacter spp. Appropriate positive and negative controls were used. Products were analysed with agarose gel electrophoresis, sequenced and results aligned using SEQUENCHER. Plasma was collected for detection of anti-Helicobacter pylori antibodies via enzyme-linked immunosorbent assay. Results: Of 36 patients, 12 patients' bile and/or tissue were positive for Helicobacter spp. by PCR. Species were most homologous with H. pylori, although other Helicobacter spp. were suggested. Six of 12 patients demonstrated anti-Helicobacter antibodies in plasma, suggesting that the remaining six might have demonstrated other species besides H. pylori. Four of six plasma samples with anti-Helicobacter antibodies were anti-CagA (cytotoxin associated gene) negative. Discussion: Helicobacter spp. can be detected in bile and gallbladder tissue of patients with benign gallbladder disease. The contribution of these bacteria to the pathophysiology of gallbladder disease and gallstone formation requires further study.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalHPB
Volume12
Issue number2
DOIs
StatePublished - Mar 2010
Externally publishedYes

Fingerprint

Helicobacter
Gallbladder Diseases
Gallbladder
Bile
Helicobacter pylori
Gallstones
Polymerase Chain Reaction
Anti-Idiotypic Antibodies
Agar Gel Electrophoresis
Cytotoxins
Cholecystectomy
Enzyme-Linked Immunosorbent Assay
Bacteria
Antibodies
DNA

Keywords

  • Cholecystitis
  • Gallbladder
  • Gallstones
  • Helicobacter

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Identification of Helicobacter spp. in bile and gallbladder tissue of patients with symptomatic gallbladder disease. / Sabbaghian, M. Shirin; Ranaudo, Jeffrey; Zeng, Lin; Alongi, Alexandra P.; Perez-Perez, Guillermo; Shamamian, Peter.

In: HPB, Vol. 12, No. 2, 03.2010, p. 129-133.

Research output: Contribution to journalArticle

Sabbaghian, M. Shirin ; Ranaudo, Jeffrey ; Zeng, Lin ; Alongi, Alexandra P. ; Perez-Perez, Guillermo ; Shamamian, Peter. / Identification of Helicobacter spp. in bile and gallbladder tissue of patients with symptomatic gallbladder disease. In: HPB. 2010 ; Vol. 12, No. 2. pp. 129-133.
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