Identification of C. elegans sensory ray genes using whole-genome expression profiling

Douglas S. Portman, Scott W. Emmons

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The three cells that comprise each C. elegans sensory ray (two sensory neurons and a structural cell) descend from a single neuroblast precursor cell. The atonal ortholog lin-32 and the E/daughterless ortholog hlh-2 act to confer neural competence during ray development, but additional regulatory factors that control specific aspects of cell fate are largely unknown. Here, we use full-genome DNA microarrays to compare gene expression profiles in adult males of two mutant strains to identify new components of the regulatory network that controls ray development and function. This approach identified a large set of candidate ray genes. Using reporter genes, we confirmed ray expression for 13 of these, including a β-tubulin, a TWK-family channel, a putative chemoreceptor and four novel genes (the cwp genes) with a potential role in sensory signaling through the C. elegans polycystins lov-1 and pkd-2. Additionally, we have found several ray-expressed transcription factors, including the Zn-finger factor egl-46 and the bHLH gene hlh-10. The expression of many of these genes requires lin-32 function, though this requirement may not reflect direct activation by lin-32. Our strategy provides a complementary foundation for modeling the genetic network that controls the development of a simple sensory organ.

Original languageEnglish (US)
Pages (from-to)499-512
Number of pages14
JournalDevelopmental Biology
Volume270
Issue number2
DOIs
StatePublished - Jun 15 2004

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Genome
Genes
Sensory Receptor Cells
Tubulin
Oligonucleotide Array Sequence Analysis
Reporter Genes
Transcriptome
Mental Competency
Fingers
Transcription Factors
Gene Expression
polycystic kidney disease 1 protein

Keywords

  • C. elegans
  • DNA microarrays
  • Genetic network
  • lin-32
  • Male tail
  • Neural subtype
  • Polycystins
  • Proneural
  • Sensory rays

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Identification of C. elegans sensory ray genes using whole-genome expression profiling. / Portman, Douglas S.; Emmons, Scott W.

In: Developmental Biology, Vol. 270, No. 2, 15.06.2004, p. 499-512.

Research output: Contribution to journalArticle

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