Identification and verification of novel rodent postsynaptic density proteins

Bryen A. Jordan, Brian D. Fernholz, Muriel Boussac, Chongfeng Xu, Gabriela Grigorean, Edward B. Ziff, Thomas A. Neubert

Research output: Contribution to journalArticle

226 Scopus citations

Abstract

The postsynaptic density (PSD) is a cellular structure specialized in receiving and transducing synaptic information. Here we describe the identification of 452 proteins isolated from biochemically purified PSD fractions of rat and mouse brains using nanoflow HPLC coupled to electrospray tandem mass spectrometry (LC-MS/MS). Fluorescence microscopy and Western blotting were used to verify that many of the novel proteins identified exhibit subcellular distributions consistent with those of PSD-localized proteins. In addition to identifying most previously described PSD components, we also detected proteins involved in signaling to the nucleus as well as regulators of ADP-ribosylation factor signaling, ubiquitination, RNA trafficking, and protein translation. These results suggest new mechanisms by which the PSD helps regulate synaptic strength and transmission.

Original languageEnglish (US)
Pages (from-to)857-871
Number of pages15
JournalMolecular and Cellular Proteomics
Volume3
Issue number9
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

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  • Cite this

    Jordan, B. A., Fernholz, B. D., Boussac, M., Xu, C., Grigorean, G., Ziff, E. B., & Neubert, T. A. (2004). Identification and verification of novel rodent postsynaptic density proteins. Molecular and Cellular Proteomics, 3(9), 857-871. https://doi.org/10.1074/mcp.M400045-MCP200