Identification and verification of novel rodent postsynaptic density proteins

Bryen A. Jordan, Brian D. Fernholz, Muriel Boussac, Chongfeng Xu, Gabriela Grigorean, Edward B. Ziff, Thomas A. Neubert

Research output: Contribution to journalArticle

219 Citations (Scopus)

Abstract

The postsynaptic density (PSD) is a cellular structure specialized in receiving and transducing synaptic information. Here we describe the identification of 452 proteins isolated from biochemically purified PSD fractions of rat and mouse brains using nanoflow HPLC coupled to electrospray tandem mass spectrometry (LC-MS/MS). Fluorescence microscopy and Western blotting were used to verify that many of the novel proteins identified exhibit subcellular distributions consistent with those of PSD-localized proteins. In addition to identifying most previously described PSD components, we also detected proteins involved in signaling to the nucleus as well as regulators of ADP-ribosylation factor signaling, ubiquitination, RNA trafficking, and protein translation. These results suggest new mechanisms by which the PSD helps regulate synaptic strength and transmission.

Original languageEnglish (US)
Pages (from-to)857-871
Number of pages15
JournalMolecular and Cellular Proteomics
Volume3
Issue number9
DOIs
StatePublished - Sep 2004
Externally publishedYes

Fingerprint

Post-Synaptic Density
Rodentia
Proteins
ADP-Ribosylation Factors
Ubiquitination
Protein Biosynthesis
Cellular Structures
Tandem Mass Spectrometry
Fluorescence Microscopy
Synaptic Transmission
Fluorescence microscopy
Western Blotting
High Pressure Liquid Chromatography
Mass spectrometry
RNA
Rats
Brain
postsynaptic density proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Jordan, B. A., Fernholz, B. D., Boussac, M., Xu, C., Grigorean, G., Ziff, E. B., & Neubert, T. A. (2004). Identification and verification of novel rodent postsynaptic density proteins. Molecular and Cellular Proteomics, 3(9), 857-871. https://doi.org/10.1074/mcp.M400045-MCP200

Identification and verification of novel rodent postsynaptic density proteins. / Jordan, Bryen A.; Fernholz, Brian D.; Boussac, Muriel; Xu, Chongfeng; Grigorean, Gabriela; Ziff, Edward B.; Neubert, Thomas A.

In: Molecular and Cellular Proteomics, Vol. 3, No. 9, 09.2004, p. 857-871.

Research output: Contribution to journalArticle

Jordan, BA, Fernholz, BD, Boussac, M, Xu, C, Grigorean, G, Ziff, EB & Neubert, TA 2004, 'Identification and verification of novel rodent postsynaptic density proteins', Molecular and Cellular Proteomics, vol. 3, no. 9, pp. 857-871. https://doi.org/10.1074/mcp.M400045-MCP200
Jordan, Bryen A. ; Fernholz, Brian D. ; Boussac, Muriel ; Xu, Chongfeng ; Grigorean, Gabriela ; Ziff, Edward B. ; Neubert, Thomas A. / Identification and verification of novel rodent postsynaptic density proteins. In: Molecular and Cellular Proteomics. 2004 ; Vol. 3, No. 9. pp. 857-871.
@article{6cdf85786a3c47b190bac81099197225,
title = "Identification and verification of novel rodent postsynaptic density proteins",
abstract = "The postsynaptic density (PSD) is a cellular structure specialized in receiving and transducing synaptic information. Here we describe the identification of 452 proteins isolated from biochemically purified PSD fractions of rat and mouse brains using nanoflow HPLC coupled to electrospray tandem mass spectrometry (LC-MS/MS). Fluorescence microscopy and Western blotting were used to verify that many of the novel proteins identified exhibit subcellular distributions consistent with those of PSD-localized proteins. In addition to identifying most previously described PSD components, we also detected proteins involved in signaling to the nucleus as well as regulators of ADP-ribosylation factor signaling, ubiquitination, RNA trafficking, and protein translation. These results suggest new mechanisms by which the PSD helps regulate synaptic strength and transmission.",
author = "Jordan, {Bryen A.} and Fernholz, {Brian D.} and Muriel Boussac and Chongfeng Xu and Gabriela Grigorean and Ziff, {Edward B.} and Neubert, {Thomas A.}",
year = "2004",
month = "9",
doi = "10.1074/mcp.M400045-MCP200",
language = "English (US)",
volume = "3",
pages = "857--871",
journal = "Molecular and Cellular Proteomics",
issn = "1535-9476",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "9",

}

TY - JOUR

T1 - Identification and verification of novel rodent postsynaptic density proteins

AU - Jordan, Bryen A.

AU - Fernholz, Brian D.

AU - Boussac, Muriel

AU - Xu, Chongfeng

AU - Grigorean, Gabriela

AU - Ziff, Edward B.

AU - Neubert, Thomas A.

PY - 2004/9

Y1 - 2004/9

N2 - The postsynaptic density (PSD) is a cellular structure specialized in receiving and transducing synaptic information. Here we describe the identification of 452 proteins isolated from biochemically purified PSD fractions of rat and mouse brains using nanoflow HPLC coupled to electrospray tandem mass spectrometry (LC-MS/MS). Fluorescence microscopy and Western blotting were used to verify that many of the novel proteins identified exhibit subcellular distributions consistent with those of PSD-localized proteins. In addition to identifying most previously described PSD components, we also detected proteins involved in signaling to the nucleus as well as regulators of ADP-ribosylation factor signaling, ubiquitination, RNA trafficking, and protein translation. These results suggest new mechanisms by which the PSD helps regulate synaptic strength and transmission.

AB - The postsynaptic density (PSD) is a cellular structure specialized in receiving and transducing synaptic information. Here we describe the identification of 452 proteins isolated from biochemically purified PSD fractions of rat and mouse brains using nanoflow HPLC coupled to electrospray tandem mass spectrometry (LC-MS/MS). Fluorescence microscopy and Western blotting were used to verify that many of the novel proteins identified exhibit subcellular distributions consistent with those of PSD-localized proteins. In addition to identifying most previously described PSD components, we also detected proteins involved in signaling to the nucleus as well as regulators of ADP-ribosylation factor signaling, ubiquitination, RNA trafficking, and protein translation. These results suggest new mechanisms by which the PSD helps regulate synaptic strength and transmission.

UR - http://www.scopus.com/inward/record.url?scp=5644266504&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=5644266504&partnerID=8YFLogxK

U2 - 10.1074/mcp.M400045-MCP200

DO - 10.1074/mcp.M400045-MCP200

M3 - Article

C2 - 15169875

AN - SCOPUS:5644266504

VL - 3

SP - 857

EP - 871

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

SN - 1535-9476

IS - 9

ER -