Identification and expression of immunogenic proteins of a disease-associated marine turtle herpesvirus

Sadie S. Coberley, Richard C. Condit, Lawrence H. Herbst, Paul A. Klein

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Herpesviruses are associated with several diseases of marine turtles, including lung-eye-trachea disease (LETD) and fibropapillomatosis. Two approaches were used to identify immunodominant antigens of LETV, the LETD-associated herpesvirus. The first approach targeted glycoprotein B, which is known to be immunogenic and neutralizing in other species. The second strategy identified LETV proteins recognized on Western blots by antibodies in immune green turtle plasma. A 38-kDa protein was resolved by two-dimensional gel electrophoresis, sequenced, and identified as a scaffolding protein encoded by the overlapping open reading frames of UL26 and UL26.5. Glycoprotein B and the scaffolding protein were cloned and expressed in Escherichia coli. The expressed proteins were recognized on Western blots by antibodies in immune green turtle plasma. Phylogenetic studies based on UL26, DNA polymerase, and glycoprotein B revealed that LETV clusters with the alphaherpesviruses.

Original languageEnglish (US)
Pages (from-to)10553-10558
Number of pages6
JournalJournal of Virology
Volume76
Issue number20
DOIs
StatePublished - Oct 2002
Externally publishedYes

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Turtles
Herpesviridae
scaffolding proteins
turtles
glycoproteins
trachea (vertebrates)
Chelonia mydas
protein synthesis
Glycoproteins
Eye Diseases
Western blotting
Trachea
eyes
lungs
Proteins
antibodies
proteins
Western Blotting
two-dimensional gel electrophoresis
DNA-directed DNA polymerase

ASJC Scopus subject areas

  • Immunology

Cite this

Identification and expression of immunogenic proteins of a disease-associated marine turtle herpesvirus. / Coberley, Sadie S.; Condit, Richard C.; Herbst, Lawrence H.; Klein, Paul A.

In: Journal of Virology, Vol. 76, No. 20, 10.2002, p. 10553-10558.

Research output: Contribution to journalArticle

Coberley, Sadie S. ; Condit, Richard C. ; Herbst, Lawrence H. ; Klein, Paul A. / Identification and expression of immunogenic proteins of a disease-associated marine turtle herpesvirus. In: Journal of Virology. 2002 ; Vol. 76, No. 20. pp. 10553-10558.
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