Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity

Ming Chieh Shun, Yair M. Botbol, Xiang Li, Francesca Di Nunzio, Janet E. Daigle, Nan Yan, Judy Lieberman, Marc Lavigne, Alan Engelman

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Lens epithelium-derived growth factor (LEDGF)/p75 functions as a bimodal tether during lentiviral DNA integration: its C-terminal integrase-binding domain interacts with the viral preintegration complex, whereas the N-terminal PWWP domain can bind to cellular chromatin. The molecular basis for the integrase-LEDGF/ p75 interaction is understood, while the mechanism of chromatin binding is unknown. The PWWP domain is homologous to other protein interaction modules that together comprise the Tudor clan. Based on primary amino acid sequence and three-dimensional structural similarities, 24 residues of the LEDGF/p75 PWWP domain were mutagenized to garner essential details of its function during human immunodeficiency virus type 1 (HIV-1) infection. Mutating either Trp-21 or Ala-51, which line the inner wall of a hydrophobic cavity that is common to Tudor clan members, disrupts chromatin binding and virus infectivity. Consistent with a role for chromatin-associated LEDGF/p75 in stimulating integrase activity during infection, recombinant W21A protein is preferentially defective for enhancing integration into chromatinized target DNA in vitro. The A51P mutation corresponds to the S270P change in DNA methyltransferase 3B that causes human immunodeficiency, centromeric instability, and facial anomaly syndrome, revealing a critical role for this amino acid position in the chromatin binding functions of varied PWWP domains. Our results furthermore highlight the requirement for a conserved Glu in the hydrophobic core that mediates interactions between other Tudor clan members and their substrates. This initial systematic mutagenesis of a PWWP domain identifies amino acid residues critical for chromatin binding function and the consequences of their changes on HIV-1 integration and infection.

Original languageEnglish (US)
Pages (from-to)11555-11567
Number of pages13
JournalJournal of Virology
Volume82
Issue number23
DOIs
StatePublished - Dec 2008
Externally publishedYes

Fingerprint

Human immunodeficiency virus 1
Lens
growth factors
Chromatin
chromatin
HIV-1
epithelium
pathogenicity
Integrases
Virus Diseases
DNA
infection
Virus Integration
Virus Attachment
Amino Acids
amino acids
immunosuppression
methyltransferases
Recombinant Proteins
recombinant proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity. / Shun, Ming Chieh; Botbol, Yair M.; Li, Xiang; Di Nunzio, Francesca; Daigle, Janet E.; Yan, Nan; Lieberman, Judy; Lavigne, Marc; Engelman, Alan.

In: Journal of Virology, Vol. 82, No. 23, 12.2008, p. 11555-11567.

Research output: Contribution to journalArticle

Shun, Ming Chieh ; Botbol, Yair M. ; Li, Xiang ; Di Nunzio, Francesca ; Daigle, Janet E. ; Yan, Nan ; Lieberman, Judy ; Lavigne, Marc ; Engelman, Alan. / Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity. In: Journal of Virology. 2008 ; Vol. 82, No. 23. pp. 11555-11567.
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AU - Di Nunzio, Francesca

AU - Daigle, Janet E.

AU - Yan, Nan

AU - Lieberman, Judy

AU - Lavigne, Marc

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