Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells

Girolama La Mantia, Domenico Maglione, Gina Pengue, Antonio Di Cristofano, Antonio Simeone, Luisa Lanfrancone, Luigi Lania

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

A novel endogenous retroviral sequence (ERV-9) has been isolated from a human embryonal carcinoma cDNA library by hybridization to a probe containing a recently described human repetitive element. DNA sequence analysis of the 4kb cDNA insert (pHE.1) revealed the presence of ORFs potentially coding for putative retrovirus-related gag, pol and env proteins. Northern blot and RNase protection experiments showed that RNA homologous Jo the pHE.1 insert is detected only in embryonal carcinoma cells as a 8 kb mRNA, and its expression is negatively regulated during retinoic acid induced differentiation of the human teratocarcinoma cell line NT2/D1. Using a pol specific probe we have isolated a genomic locus containing the ERV-9 sequences. Characterization by restriction enzyme analysis and DNA sequencing allowed us to define LTR-like sequences, that are composed by a complex array of subrepetitive elements. In addition we show that ERV-9 LTR sequences are capable to drive expression of linked CAT gene in a cell specific manner as LTR promoter activity has been detected only in NT2/D1 cells.

Original languageEnglish (US)
Pages (from-to)1513-1520
Number of pages8
JournalNucleic Acids Research
Volume19
Issue number7
StatePublished - Apr 11 1991
Externally publishedYes

Fingerprint

Endogenous Retroviruses
Embryonal Carcinoma Stem Cells
gag-pol Fusion Proteins
Cells
env Gene Products
Carcinoma
DNA Sequence Analysis
DNA sequences
DNA Restriction Enzymes
Cell
Ribonucleases
CDNA
Tretinoin
RNA
Gene Library
Embryonal Carcinoma
Teratocarcinoma
Restriction Mapping
DNA
Probe

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells. / La Mantia, Girolama; Maglione, Domenico; Pengue, Gina; Di Cristofano, Antonio; Simeone, Antonio; Lanfrancone, Luisa; Lania, Luigi.

In: Nucleic Acids Research, Vol. 19, No. 7, 11.04.1991, p. 1513-1520.

Research output: Contribution to journalArticle

La Mantia, Girolama ; Maglione, Domenico ; Pengue, Gina ; Di Cristofano, Antonio ; Simeone, Antonio ; Lanfrancone, Luisa ; Lania, Luigi. / Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells. In: Nucleic Acids Research. 1991 ; Vol. 19, No. 7. pp. 1513-1520.
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