Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells

Girolama La Mantia; Domenico Maglione; Gina Pengue; Antonio Di Cristofano; Antonio Simeone; Luisa Lanfrancone; Luigi Lania

doi:10.1093/nar/19.7.1513

Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells

Girolama La Mantia, Domenico Maglione, Gina Pengue, Antonio Di Cristofano, Antonio Simeone, Luisa Lanfrancone, Luigi Lania

Research output: Contribution to journal › Article › peer-review

104 Scopus citations

Abstract

A novel endogenous retroviral sequence (ERV-9) has been isolated from a human embryonal carcinoma cDNA library by hybridization to a probe containing a recently described human repetitive element. DNA sequence analysis of the 4kb cDNA insert (pHE.1) revealed the presence of ORFs potentially coding for putative retrovirus-related gag, pol and env proteins. Northern blot and RNase protection experiments showed that RNA homologous to the pHE.1 insert is detected only in embryonal carcinoma cells as a 8 kb mRNA, and its expression is negatively regulated during retinoic acid induced differentiation of the human teratocarcinoma cell line NT2/D1. Using a pol specific probe we have isolated a genomic locus containing the ERV-9 sequences. Characterization by restriction enzyme analysis and DNA sequencing allowed us to define LTR-like sequences, that are composed by a complex array of subrepetitive elements. In addition we show that ERV-9 LTR sequences are capable to drive expression of linked CAT gene in a cell specific manner as LTR promoter activity has been detected only in NT2/D1 cells.

Original language	English (US)
Pages (from-to)	1513-1520
Number of pages	8
Journal	Nucleic acids research
Volume	19
Issue number	7
DOIs	https://doi.org/10.1093/nar/19.7.1513
State	Published - Apr 11 1991
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells",

abstract = "A novel endogenous retroviral sequence (ERV-9) has been isolated from a human embryonal carcinoma cDNA library by hybridization to a probe containing a recently described human repetitive element. DNA sequence analysis of the 4kb cDNA insert (pHE.1) revealed the presence of ORFs potentially coding for putative retrovirus-related gag, pol and env proteins. Northern blot and RNase protection experiments showed that RNA homologous to the pHE.1 insert is detected only in embryonal carcinoma cells as a 8 kb mRNA, and its expression is negatively regulated during retinoic acid induced differentiation of the human teratocarcinoma cell line NT2/D1. Using a pol specific probe we have isolated a genomic locus containing the ERV-9 sequences. Characterization by restriction enzyme analysis and DNA sequencing allowed us to define LTR-like sequences, that are composed by a complex array of subrepetitive elements. In addition we show that ERV-9 LTR sequences are capable to drive expression of linked CAT gene in a cell specific manner as LTR promoter activity has been detected only in NT2/D1 cells.",

author = "Mantia, {Girolama La} and Domenico Maglione and Gina Pengue and Cristofano, {Antonio Di} and Antonio Simeone and Luisa Lanfrancone and Luigi Lania",

note = "Funding Information: We would like to thank R. Terracciano for skilful technical help, and E. Boncinelli and P.P. Di Nocera for helpful discussions. This work was paid for by grants from the Italian Association for Cancer Research (AIRC) to L.L., MPI (40%) to G.L.M. and from grant n° 5206072 from Progetto AIDS. G.P. is recipient of an AIRC fellowship.",

year = "1991",

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doi = "10.1093/nar/19.7.1513",

language = "English (US)",

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T1 - Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells

AU - Mantia, Girolama La

AU - Maglione, Domenico

AU - Pengue, Gina

AU - Cristofano, Antonio Di

AU - Simeone, Antonio

AU - Lanfrancone, Luisa

AU - Lania, Luigi

N1 - Funding Information: We would like to thank R. Terracciano for skilful technical help, and E. Boncinelli and P.P. Di Nocera for helpful discussions. This work was paid for by grants from the Italian Association for Cancer Research (AIRC) to L.L., MPI (40%) to G.L.M. and from grant n° 5206072 from Progetto AIDS. G.P. is recipient of an AIRC fellowship.

PY - 1991/4/11

Y1 - 1991/4/11

N2 - A novel endogenous retroviral sequence (ERV-9) has been isolated from a human embryonal carcinoma cDNA library by hybridization to a probe containing a recently described human repetitive element. DNA sequence analysis of the 4kb cDNA insert (pHE.1) revealed the presence of ORFs potentially coding for putative retrovirus-related gag, pol and env proteins. Northern blot and RNase protection experiments showed that RNA homologous to the pHE.1 insert is detected only in embryonal carcinoma cells as a 8 kb mRNA, and its expression is negatively regulated during retinoic acid induced differentiation of the human teratocarcinoma cell line NT2/D1. Using a pol specific probe we have isolated a genomic locus containing the ERV-9 sequences. Characterization by restriction enzyme analysis and DNA sequencing allowed us to define LTR-like sequences, that are composed by a complex array of subrepetitive elements. In addition we show that ERV-9 LTR sequences are capable to drive expression of linked CAT gene in a cell specific manner as LTR promoter activity has been detected only in NT2/D1 cells.

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Identification and characterization of novel human endogenous retroviral sequences prefentially expressed in undifferentiated embryonal carcinoma cells

Abstract

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