Identification and Characterization of Bacterial Vaginosis-Associated Pathogens Using a Comprehensive Cervical-Vaginal Epithelial Coculture Assay

Colleen R. Eade, Camila Diaz, Matthew P. Wood, Kathryn Anastos, Bruce K. Patterson, Phalguni Gupta, Amy L. Cole, Alexander M. Cole

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Bacterial vaginosis (BV) is the most commonly treated female reproductive tract affliction, characterized by the displacement of healthy lactobacilli by an overgrowth of pathogenic bacteria. BV can contribute to pathogenic inflammation, preterm birth, and susceptibility to sexually transmitted infections. As the bacteria responsible for BV pathogenicity and their interactions with host immunity are not understood, we sought to evaluate the effects of BV-associated bacteria on reproductive epithelia. Here we have characterized the interaction between BV-associated bacteria and the female reproductive tract by measuring cytokine and defensin induction in three types of FRT epithelial cells following bacterial inoculation. Four BV-associated bacteria were evaluated alongside six lactobacilli for a comparative assessment. While responses differed between epithelial cell types, our model showed good agreement with clinical BV trends. We observed a distinct cytokine and human β-defensin 2 response to BV-associated bacteria, especially Atopobium vaginae, compared to most lactobacilli. One lactobacillus species, Lactobacillus vaginalis, induced an immune response similar to that elicited by BV-associated bacteria, stimulating significantly higher levels of cytokines and human β-defensin 2 than other lactobacilli. These data provide an important prioritization of BV-associated bacteria and support further characterization of reproductive bacteria and their interactions with host epithelia. Additionally, they demonstrate the distinct immune response potentials of epithelial cells from different locations along the female reproductive tract.

Original languageEnglish (US)
Article numbere50106
JournalPLoS One
Volume7
Issue number11
DOIs
StatePublished - Nov 15 2012

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Bacterial Vaginosis
Pathogens
coculture
Coculture Techniques
Assays
Bacteria
Lactobacillus
pathogens
bacteria
assays
Defensins
epithelial cells
cytokines
Cytokines
Epithelial Cells
Atopobium vaginae
Lactobacillus vaginalis
epithelium
immune response
Epithelium

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Identification and Characterization of Bacterial Vaginosis-Associated Pathogens Using a Comprehensive Cervical-Vaginal Epithelial Coculture Assay. / Eade, Colleen R.; Diaz, Camila; Wood, Matthew P.; Anastos, Kathryn; Patterson, Bruce K.; Gupta, Phalguni; Cole, Amy L.; Cole, Alexander M.

In: PLoS One, Vol. 7, No. 11, e50106, 15.11.2012.

Research output: Contribution to journalArticle

Eade, Colleen R. ; Diaz, Camila ; Wood, Matthew P. ; Anastos, Kathryn ; Patterson, Bruce K. ; Gupta, Phalguni ; Cole, Amy L. ; Cole, Alexander M. / Identification and Characterization of Bacterial Vaginosis-Associated Pathogens Using a Comprehensive Cervical-Vaginal Epithelial Coculture Assay. In: PLoS One. 2012 ; Vol. 7, No. 11.
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