Hyperglycemia impairs proteasome function by methylglyoxal

Markus A. Queisser, Dachun Yao, Sven Geisler, Hans Peter Hammes, Günter Lochnit, Erwin D. Schleicher, Michael Brownlee, Klaus T. Preissner

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

OBJECTIVE - The ubiquitin-proteasome system is the main degradation machinery for intracellularly altered proteins. Hyperglycemia has been shown to increase intracellular levels of the reactive dicarbonyl methylglyoxal (MGO) in cells damaged by diabetes, resulting in modification of proteins and alterations of their function. In this study, the influence of MGO-derived advanced glycation end product (AGE) formation on the activity of the proteasome was investigated in vitro and in vivo. RESEARCH DESIGN AND METHODS - MGO-derived AGE modification of proteasome subunits was analyzed by mass spectrometry, immunoprecipitation, and Western blots. Proteasome activity was analyzed using proteasome-specific fluorogenic substrates. Experimental models included bovine retinal endothelial cells, diabetic Ins2Akita mice, glyoxalase 1 (GLO1) knockdown mice, and streptozotocin (STZ)-injected diabetic mice. RESULTS - In vitro incubation with MGO caused adduct formation on several 20S proteasomal subunit proteins. In cultured endothelial cells, the expression level of the catalytic 20S proteasome subunit was not altered but proteasomal chymotrypsinlike activity was significantly reduced. In contrast, levels of regulatory 19S proteasomal proteins were decreased. In diabetic Ins2 Akita, STZ diabetic, and nondiabetic and diabetic G101 knockdown mice, chymotrypsin-like activity was also reduced and MGO modification of the 20S-β2 subunit was increased. CONCLUSIONS - Hyperglycemia-induced formation of MGO covalently modifies the 20S proteasome, decreasing its activity in the diabetic kidney and reducing the polyubiquitin receptor 19S-S5a. The results indicate a new link between hyperglycemia and impairment of cell functions.

Original languageEnglish (US)
Pages (from-to)670-678
Number of pages9
JournalDiabetes
Volume59
Issue number3
DOIs
StatePublished - Mar 2010

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Pyruvaldehyde
Proteasome Endopeptidase Complex
Hyperglycemia
Advanced Glycosylation End Products
Streptozocin
Endothelial Cells
Polyubiquitin
Proteins
Protein Subunits
Chymotrypsin
Ubiquitin
Fluorescent Dyes
Immunoprecipitation
Cultured Cells
Mass Spectrometry
Research Design
Theoretical Models
Western Blotting
Kidney

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Queisser, M. A., Yao, D., Geisler, S., Hammes, H. P., Lochnit, G., Schleicher, E. D., ... Preissner, K. T. (2010). Hyperglycemia impairs proteasome function by methylglyoxal. Diabetes, 59(3), 670-678. https://doi.org/10.2337/db08-1565

Hyperglycemia impairs proteasome function by methylglyoxal. / Queisser, Markus A.; Yao, Dachun; Geisler, Sven; Hammes, Hans Peter; Lochnit, Günter; Schleicher, Erwin D.; Brownlee, Michael; Preissner, Klaus T.

In: Diabetes, Vol. 59, No. 3, 03.2010, p. 670-678.

Research output: Contribution to journalArticle

Queisser, MA, Yao, D, Geisler, S, Hammes, HP, Lochnit, G, Schleicher, ED, Brownlee, M & Preissner, KT 2010, 'Hyperglycemia impairs proteasome function by methylglyoxal', Diabetes, vol. 59, no. 3, pp. 670-678. https://doi.org/10.2337/db08-1565
Queisser MA, Yao D, Geisler S, Hammes HP, Lochnit G, Schleicher ED et al. Hyperglycemia impairs proteasome function by methylglyoxal. Diabetes. 2010 Mar;59(3):670-678. https://doi.org/10.2337/db08-1565
Queisser, Markus A. ; Yao, Dachun ; Geisler, Sven ; Hammes, Hans Peter ; Lochnit, Günter ; Schleicher, Erwin D. ; Brownlee, Michael ; Preissner, Klaus T. / Hyperglycemia impairs proteasome function by methylglyoxal. In: Diabetes. 2010 ; Vol. 59, No. 3. pp. 670-678.
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