Hyaluronan modifies inflammatory response and peritoneal permeability during peritonitis in rats

Andrzej Breborowicz, Alicja Polubinska, James Moberly, Kristen Ogle, Leo Martis, Dimitrios Oreopoulos

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The effect of high-molecular-weight hyaluronan (HA) on peritoneal and systemic inflammation and peritoneal permeability to water and solutes was studied during endotoxin-induced peritonitis in rats. Acute peritonitis was induced by adding lipopolysaccharide (LPS) to the dialysis fluid (Dianeal 3.86; Baxter Healthcare, Ireland, Castlebar). HA was added to the dialysis solution in a concentration of 10 mg/dL. During 4- and 8-hour dwells of the dialysis fluid, we studied the intensity of peritoneal (dialysate) and systemic (blood) inflammation (dialysate cell count and differential, cytokine and HA levels), as well as the transperitoneal transport of solutes and water. In rats, the addition of LPS to the dialysis fluid induced changes in inflammatory reaction and transperitoneal transport similar to those seen in continuous ambulatory peritoneal dialysis patients with peritonitis. During peritonitis, the addition of HA to the dialysis fluid reduced the loss of ultrafiltration, which resulted in a greater peritoneal creatinine clearance during the 8 hours of dwell (29.9 ± 6.7 mL/8 h in the HA-LPS group versus 19.7 ± 7.8 mL/8 h in the LPS group; P < 0.05). Dialysate interferon-γ (INF-γ) levels during peritonitis were greater in HA-treated animals (536.8 ± 296.6 pg/mL in the HA-LPS group versus 169.8 ± 137.8 pg/mL in the LPS group; P < 0.05). Dialysate elastase activity increased during peritonitis (44.4 ± 9.3 versus 14.2 ± 4.1 U/mL in peritonitis-free rats); during peritonitis, the increase in dialysate elastase activity was less pronounced in the rats that had HA in the dialysate (27.3 ± 4.1 U/mL versus the LPS group; P < 0.01). We conclude that HA added to the dialysis fluid reduces loss of ultrafiltration during peritonitis in rats. In the presence of HA dialysate, INF-γ levels during peritonitis increased, whereas elastase activity decreased; these changes might improve the peritoneal immune reaction during peritonitis and at the same time prevent peritoneal membrane injury.

Original languageEnglish (US)
Pages (from-to)594-600
Number of pages7
JournalAmerican Journal of Kidney Diseases
Volume37
Issue number3
StatePublished - 2001
Externally publishedYes

Fingerprint

Hyaluronic Acid
Peritonitis
Permeability
Dialysis Solutions
Lipopolysaccharides
Dialysis
Pancreatic Elastase
Ultrafiltration
Interferons
Inflammation
Continuous Ambulatory Peritoneal Dialysis
Water
Ireland
Endotoxins
Creatinine
Cell Count
Molecular Weight
Cytokines
Delivery of Health Care
Membranes

Keywords

  • Hyaluronan
  • Peritoneal dialysis (PD)
  • Peritonitis
  • Rats

ASJC Scopus subject areas

  • Nephrology

Cite this

Breborowicz, A., Polubinska, A., Moberly, J., Ogle, K., Martis, L., & Oreopoulos, D. (2001). Hyaluronan modifies inflammatory response and peritoneal permeability during peritonitis in rats. American Journal of Kidney Diseases, 37(3), 594-600.

Hyaluronan modifies inflammatory response and peritoneal permeability during peritonitis in rats. / Breborowicz, Andrzej; Polubinska, Alicja; Moberly, James; Ogle, Kristen; Martis, Leo; Oreopoulos, Dimitrios.

In: American Journal of Kidney Diseases, Vol. 37, No. 3, 2001, p. 594-600.

Research output: Contribution to journalArticle

Breborowicz, A, Polubinska, A, Moberly, J, Ogle, K, Martis, L & Oreopoulos, D 2001, 'Hyaluronan modifies inflammatory response and peritoneal permeability during peritonitis in rats', American Journal of Kidney Diseases, vol. 37, no. 3, pp. 594-600.
Breborowicz A, Polubinska A, Moberly J, Ogle K, Martis L, Oreopoulos D. Hyaluronan modifies inflammatory response and peritoneal permeability during peritonitis in rats. American Journal of Kidney Diseases. 2001;37(3):594-600.
Breborowicz, Andrzej ; Polubinska, Alicja ; Moberly, James ; Ogle, Kristen ; Martis, Leo ; Oreopoulos, Dimitrios. / Hyaluronan modifies inflammatory response and peritoneal permeability during peritonitis in rats. In: American Journal of Kidney Diseases. 2001 ; Vol. 37, No. 3. pp. 594-600.
@article{e9585cf0c6ae43308b5c9b209abfd2c3,
title = "Hyaluronan modifies inflammatory response and peritoneal permeability during peritonitis in rats",
abstract = "The effect of high-molecular-weight hyaluronan (HA) on peritoneal and systemic inflammation and peritoneal permeability to water and solutes was studied during endotoxin-induced peritonitis in rats. Acute peritonitis was induced by adding lipopolysaccharide (LPS) to the dialysis fluid (Dianeal 3.86; Baxter Healthcare, Ireland, Castlebar). HA was added to the dialysis solution in a concentration of 10 mg/dL. During 4- and 8-hour dwells of the dialysis fluid, we studied the intensity of peritoneal (dialysate) and systemic (blood) inflammation (dialysate cell count and differential, cytokine and HA levels), as well as the transperitoneal transport of solutes and water. In rats, the addition of LPS to the dialysis fluid induced changes in inflammatory reaction and transperitoneal transport similar to those seen in continuous ambulatory peritoneal dialysis patients with peritonitis. During peritonitis, the addition of HA to the dialysis fluid reduced the loss of ultrafiltration, which resulted in a greater peritoneal creatinine clearance during the 8 hours of dwell (29.9 ± 6.7 mL/8 h in the HA-LPS group versus 19.7 ± 7.8 mL/8 h in the LPS group; P < 0.05). Dialysate interferon-γ (INF-γ) levels during peritonitis were greater in HA-treated animals (536.8 ± 296.6 pg/mL in the HA-LPS group versus 169.8 ± 137.8 pg/mL in the LPS group; P < 0.05). Dialysate elastase activity increased during peritonitis (44.4 ± 9.3 versus 14.2 ± 4.1 U/mL in peritonitis-free rats); during peritonitis, the increase in dialysate elastase activity was less pronounced in the rats that had HA in the dialysate (27.3 ± 4.1 U/mL versus the LPS group; P < 0.01). We conclude that HA added to the dialysis fluid reduces loss of ultrafiltration during peritonitis in rats. In the presence of HA dialysate, INF-γ levels during peritonitis increased, whereas elastase activity decreased; these changes might improve the peritoneal immune reaction during peritonitis and at the same time prevent peritoneal membrane injury.",
keywords = "Hyaluronan, Peritoneal dialysis (PD), Peritonitis, Rats",
author = "Andrzej Breborowicz and Alicja Polubinska and James Moberly and Kristen Ogle and Leo Martis and Dimitrios Oreopoulos",
year = "2001",
language = "English (US)",
volume = "37",
pages = "594--600",
journal = "American Journal of Kidney Diseases",
issn = "0272-6386",
publisher = "W.B. Saunders Ltd",
number = "3",

}

TY - JOUR

T1 - Hyaluronan modifies inflammatory response and peritoneal permeability during peritonitis in rats

AU - Breborowicz, Andrzej

AU - Polubinska, Alicja

AU - Moberly, James

AU - Ogle, Kristen

AU - Martis, Leo

AU - Oreopoulos, Dimitrios

PY - 2001

Y1 - 2001

N2 - The effect of high-molecular-weight hyaluronan (HA) on peritoneal and systemic inflammation and peritoneal permeability to water and solutes was studied during endotoxin-induced peritonitis in rats. Acute peritonitis was induced by adding lipopolysaccharide (LPS) to the dialysis fluid (Dianeal 3.86; Baxter Healthcare, Ireland, Castlebar). HA was added to the dialysis solution in a concentration of 10 mg/dL. During 4- and 8-hour dwells of the dialysis fluid, we studied the intensity of peritoneal (dialysate) and systemic (blood) inflammation (dialysate cell count and differential, cytokine and HA levels), as well as the transperitoneal transport of solutes and water. In rats, the addition of LPS to the dialysis fluid induced changes in inflammatory reaction and transperitoneal transport similar to those seen in continuous ambulatory peritoneal dialysis patients with peritonitis. During peritonitis, the addition of HA to the dialysis fluid reduced the loss of ultrafiltration, which resulted in a greater peritoneal creatinine clearance during the 8 hours of dwell (29.9 ± 6.7 mL/8 h in the HA-LPS group versus 19.7 ± 7.8 mL/8 h in the LPS group; P < 0.05). Dialysate interferon-γ (INF-γ) levels during peritonitis were greater in HA-treated animals (536.8 ± 296.6 pg/mL in the HA-LPS group versus 169.8 ± 137.8 pg/mL in the LPS group; P < 0.05). Dialysate elastase activity increased during peritonitis (44.4 ± 9.3 versus 14.2 ± 4.1 U/mL in peritonitis-free rats); during peritonitis, the increase in dialysate elastase activity was less pronounced in the rats that had HA in the dialysate (27.3 ± 4.1 U/mL versus the LPS group; P < 0.01). We conclude that HA added to the dialysis fluid reduces loss of ultrafiltration during peritonitis in rats. In the presence of HA dialysate, INF-γ levels during peritonitis increased, whereas elastase activity decreased; these changes might improve the peritoneal immune reaction during peritonitis and at the same time prevent peritoneal membrane injury.

AB - The effect of high-molecular-weight hyaluronan (HA) on peritoneal and systemic inflammation and peritoneal permeability to water and solutes was studied during endotoxin-induced peritonitis in rats. Acute peritonitis was induced by adding lipopolysaccharide (LPS) to the dialysis fluid (Dianeal 3.86; Baxter Healthcare, Ireland, Castlebar). HA was added to the dialysis solution in a concentration of 10 mg/dL. During 4- and 8-hour dwells of the dialysis fluid, we studied the intensity of peritoneal (dialysate) and systemic (blood) inflammation (dialysate cell count and differential, cytokine and HA levels), as well as the transperitoneal transport of solutes and water. In rats, the addition of LPS to the dialysis fluid induced changes in inflammatory reaction and transperitoneal transport similar to those seen in continuous ambulatory peritoneal dialysis patients with peritonitis. During peritonitis, the addition of HA to the dialysis fluid reduced the loss of ultrafiltration, which resulted in a greater peritoneal creatinine clearance during the 8 hours of dwell (29.9 ± 6.7 mL/8 h in the HA-LPS group versus 19.7 ± 7.8 mL/8 h in the LPS group; P < 0.05). Dialysate interferon-γ (INF-γ) levels during peritonitis were greater in HA-treated animals (536.8 ± 296.6 pg/mL in the HA-LPS group versus 169.8 ± 137.8 pg/mL in the LPS group; P < 0.05). Dialysate elastase activity increased during peritonitis (44.4 ± 9.3 versus 14.2 ± 4.1 U/mL in peritonitis-free rats); during peritonitis, the increase in dialysate elastase activity was less pronounced in the rats that had HA in the dialysate (27.3 ± 4.1 U/mL versus the LPS group; P < 0.01). We conclude that HA added to the dialysis fluid reduces loss of ultrafiltration during peritonitis in rats. In the presence of HA dialysate, INF-γ levels during peritonitis increased, whereas elastase activity decreased; these changes might improve the peritoneal immune reaction during peritonitis and at the same time prevent peritoneal membrane injury.

KW - Hyaluronan

KW - Peritoneal dialysis (PD)

KW - Peritonitis

KW - Rats

UR - http://www.scopus.com/inward/record.url?scp=0035123653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035123653&partnerID=8YFLogxK

M3 - Article

C2 - 11228185

AN - SCOPUS:0035123653

VL - 37

SP - 594

EP - 600

JO - American Journal of Kidney Diseases

JF - American Journal of Kidney Diseases

SN - 0272-6386

IS - 3

ER -