Human liver cell transplantation. Prolonged function in Athymic-Gunn and athymic-analbuminemic hybrid rats

Albert D. Moscioni, Jayanta Roy-Chowdhury, Ronald Barbour, Laurel L. Brown, Namita Roy-Chowdhury, Louis S. Competiello, Pulak Lahiri, Achilles A. Demetriou

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Isolated cryopreserved human liver cells, attached to collagen-coated microcarriers, were injected intraperitoneally into mutant rat recipients genetically deficient in either bilirubin uridine diphosphoglucuronosyltransferase activity (Gunn rats) or albumin synthesis (Nagase analbuminemic rats). One group of the recipient Gunn and analbuminemic rats were made genetically immunodeficient by interbreeding with athymic rats with inherited T-cell deficiency. Injected microcarriers and cells formed aggregates on the surface of the pancreas. There was no morphologic evidence of rejection in athymic recipients, whereas immunocompetent recipients demonstrated rejection within 5 days of transplantation. Athymic-Gunn rat recipients demonstrated excretion of bilirubin glucuronides in bile for 30 days and reduction in their serum bilirubin levels. In recipient athymic-analbuminemic rats, plasma albumin levels increased from pretransplantation levels of 0.025-0.05 mg/ml to 3.9-4.8 mg/ml 8 days posttransplantation and remained nearly at that level throughout the 30 days of the study. A method of harvesting, attaching to microcarriers, cryopreserving, and in vivo testing of human hepatocytes with prolonged survival and function in athymic-Gunn and athymic-analbuminemic hybrid rats is reported. These rats are excellent animal models for testing xenograft function.

Original languageEnglish (US)
Pages (from-to)1546-1551
Number of pages6
JournalGastroenterology
Volume96
Issue number6
StatePublished - 1989

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Gunn Rats
Cell Transplantation
Nude Rats
Liver Transplantation
Bilirubin
Acetylglucosaminidase
Uridine
Heterografts
Bile
Serum Albumin
Pancreas
Hepatocytes
Albumins
Collagen
Animal Models
Transplantation
T-Lymphocytes
Survival
Liver
Serum

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Moscioni, A. D., Roy-Chowdhury, J., Barbour, R., Brown, L. L., Roy-Chowdhury, N., Competiello, L. S., ... Demetriou, A. A. (1989). Human liver cell transplantation. Prolonged function in Athymic-Gunn and athymic-analbuminemic hybrid rats. Gastroenterology, 96(6), 1546-1551.

Human liver cell transplantation. Prolonged function in Athymic-Gunn and athymic-analbuminemic hybrid rats. / Moscioni, Albert D.; Roy-Chowdhury, Jayanta; Barbour, Ronald; Brown, Laurel L.; Roy-Chowdhury, Namita; Competiello, Louis S.; Lahiri, Pulak; Demetriou, Achilles A.

In: Gastroenterology, Vol. 96, No. 6, 1989, p. 1546-1551.

Research output: Contribution to journalArticle

Moscioni, AD, Roy-Chowdhury, J, Barbour, R, Brown, LL, Roy-Chowdhury, N, Competiello, LS, Lahiri, P & Demetriou, AA 1989, 'Human liver cell transplantation. Prolonged function in Athymic-Gunn and athymic-analbuminemic hybrid rats', Gastroenterology, vol. 96, no. 6, pp. 1546-1551.
Moscioni AD, Roy-Chowdhury J, Barbour R, Brown LL, Roy-Chowdhury N, Competiello LS et al. Human liver cell transplantation. Prolonged function in Athymic-Gunn and athymic-analbuminemic hybrid rats. Gastroenterology. 1989;96(6):1546-1551.
Moscioni, Albert D. ; Roy-Chowdhury, Jayanta ; Barbour, Ronald ; Brown, Laurel L. ; Roy-Chowdhury, Namita ; Competiello, Louis S. ; Lahiri, Pulak ; Demetriou, Achilles A. / Human liver cell transplantation. Prolonged function in Athymic-Gunn and athymic-analbuminemic hybrid rats. In: Gastroenterology. 1989 ; Vol. 96, No. 6. pp. 1546-1551.
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