Human antibodies elicited by a pneumococcal vaccine express ldiotypic determinants indicative of V(H)3 gene segment usage

Jacobo Abadi, Joseph Friedman, Rizgar A. Mageed, Roy Jefferis, Maria C. Rodriguez-Barradas, Liise-anne Pirofski

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Human immunodeficiency virus (HIV)-infected persons manifest decreased antibody responses to pneumococcal polysaccharide vaccines. Since human antibody responses to polysaccharides are often restricted, the molecular structure of antibodies elicited by a 23-valent pneumococcal vaccine was analyzed. Anti-idiotypic reagents were used to detect V(H)1, V(H)3, and V(H)4 gene usage by antibodies to pneumococcal capsular polysaccharides in HIV- uninfected and HIV-infected subjects by ELISA. HIV-uninfected persons generated β-mercaptoethanol-sensitive and -resistant antibodies to pneumococcal capsular polysaccharides expressing V(H)3 determinants recognized by the D12, 16.84, and B6 monoclonal antibodies; antibodies expressing V(H)1 determinants were not detected, and V(H)4 determinants were expressed by β-mercaptoethanol-sensitive antibodies only; and HIV-infected subjects had significantly lower capsular polysaccharide-specific and V(H)3- positive antibody responses. These findings confirm decreased antibody responses to pneumococcal vaccination in HIV-infected persons and suggest that their poor responses may result from HIV-associated depletion of restricted B cell subsets.

Original languageEnglish (US)
Pages (from-to)707-716
Number of pages10
JournalJournal of Infectious Diseases
Volume178
Issue number3
StatePublished - 1998

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Pneumococcal Vaccines
HIV
Antibodies
Antibody Formation
Polysaccharides
Genes
Mercaptoethanol
B-Lymphocyte Subsets
Molecular Structure
Vaccination
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Human antibodies elicited by a pneumococcal vaccine express ldiotypic determinants indicative of V(H)3 gene segment usage. / Abadi, Jacobo; Friedman, Joseph; Mageed, Rizgar A.; Jefferis, Roy; Rodriguez-Barradas, Maria C.; Pirofski, Liise-anne.

In: Journal of Infectious Diseases, Vol. 178, No. 3, 1998, p. 707-716.

Research output: Contribution to journalArticle

Abadi, J, Friedman, J, Mageed, RA, Jefferis, R, Rodriguez-Barradas, MC & Pirofski, L 1998, 'Human antibodies elicited by a pneumococcal vaccine express ldiotypic determinants indicative of V(H)3 gene segment usage', Journal of Infectious Diseases, vol. 178, no. 3, pp. 707-716.
Abadi, Jacobo ; Friedman, Joseph ; Mageed, Rizgar A. ; Jefferis, Roy ; Rodriguez-Barradas, Maria C. ; Pirofski, Liise-anne. / Human antibodies elicited by a pneumococcal vaccine express ldiotypic determinants indicative of V(H)3 gene segment usage. In: Journal of Infectious Diseases. 1998 ; Vol. 178, No. 3. pp. 707-716.
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