Human antibodies elicited by a pneumococcal vaccine express ldiotypic determinants indicative of V(H)3 gene segment usage

Jacobo Abadi, Joseph Friedman, Rizgar A. Mageed, Roy Jefferis, Maria C. Rodriguez-Barradas, Liise Anne Pirofski

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Abstract

Human immunodeficiency virus (HIV)-infected persons manifest decreased antibody responses to pneumococcal polysaccharide vaccines. Since human antibody responses to polysaccharides are often restricted, the molecular structure of antibodies elicited by a 23-valent pneumococcal vaccine was analyzed. Anti-idiotypic reagents were used to detect V(H)1, V(H)3, and V(H)4 gene usage by antibodies to pneumococcal capsular polysaccharides in HIV- uninfected and HIV-infected subjects by ELISA. HIV-uninfected persons generated β-mercaptoethanol-sensitive and -resistant antibodies to pneumococcal capsular polysaccharides expressing V(H)3 determinants recognized by the D12, 16.84, and B6 monoclonal antibodies; antibodies expressing V(H)1 determinants were not detected, and V(H)4 determinants were expressed by β-mercaptoethanol-sensitive antibodies only; and HIV-infected subjects had significantly lower capsular polysaccharide-specific and V(H)3- positive antibody responses. These findings confirm decreased antibody responses to pneumococcal vaccination in HIV-infected persons and suggest that their poor responses may result from HIV-associated depletion of restricted B cell subsets.

Original languageEnglish (US)
Pages (from-to)707-716
Number of pages10
JournalJournal of Infectious Diseases
Volume178
Issue number3
DOIs
Publication statusPublished - Jan 1 1998

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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