TY - JOUR
T1 - Human antibodies elicited by a pneumococcal vaccine express ldiotypic determinants indicative of V(H)3 gene segment usage
AU - Abadi, Jacobo
AU - Friedman, Joseph
AU - Mageed, Rizgar A.
AU - Jefferis, Roy
AU - Rodriguez-Barradas, Maria C.
AU - Pirofski, Liise Anne
PY - 1998
Y1 - 1998
N2 - Human immunodeficiency virus (HIV)-infected persons manifest decreased antibody responses to pneumococcal polysaccharide vaccines. Since human antibody responses to polysaccharides are often restricted, the molecular structure of antibodies elicited by a 23-valent pneumococcal vaccine was analyzed. Anti-idiotypic reagents were used to detect V(H)1, V(H)3, and V(H)4 gene usage by antibodies to pneumococcal capsular polysaccharides in HIV- uninfected and HIV-infected subjects by ELISA. HIV-uninfected persons generated β-mercaptoethanol-sensitive and -resistant antibodies to pneumococcal capsular polysaccharides expressing V(H)3 determinants recognized by the D12, 16.84, and B6 monoclonal antibodies; antibodies expressing V(H)1 determinants were not detected, and V(H)4 determinants were expressed by β-mercaptoethanol-sensitive antibodies only; and HIV-infected subjects had significantly lower capsular polysaccharide-specific and V(H)3- positive antibody responses. These findings confirm decreased antibody responses to pneumococcal vaccination in HIV-infected persons and suggest that their poor responses may result from HIV-associated depletion of restricted B cell subsets.
AB - Human immunodeficiency virus (HIV)-infected persons manifest decreased antibody responses to pneumococcal polysaccharide vaccines. Since human antibody responses to polysaccharides are often restricted, the molecular structure of antibodies elicited by a 23-valent pneumococcal vaccine was analyzed. Anti-idiotypic reagents were used to detect V(H)1, V(H)3, and V(H)4 gene usage by antibodies to pneumococcal capsular polysaccharides in HIV- uninfected and HIV-infected subjects by ELISA. HIV-uninfected persons generated β-mercaptoethanol-sensitive and -resistant antibodies to pneumococcal capsular polysaccharides expressing V(H)3 determinants recognized by the D12, 16.84, and B6 monoclonal antibodies; antibodies expressing V(H)1 determinants were not detected, and V(H)4 determinants were expressed by β-mercaptoethanol-sensitive antibodies only; and HIV-infected subjects had significantly lower capsular polysaccharide-specific and V(H)3- positive antibody responses. These findings confirm decreased antibody responses to pneumococcal vaccination in HIV-infected persons and suggest that their poor responses may result from HIV-associated depletion of restricted B cell subsets.
UR - http://www.scopus.com/inward/record.url?scp=0031658738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031658738&partnerID=8YFLogxK
U2 - 10.1086/515369
DO - 10.1086/515369
M3 - Article
C2 - 9728539
AN - SCOPUS:0031658738
SN - 0022-1899
VL - 178
SP - 707
EP - 716
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 3
ER -