HR22C16

A potent small-molecule probe for the dynamics of cell division

Srinivas Hotha, Justin C. Yarrow, Janet G. Yang, Sarah C. Garrett, Kishore V. Renduchintala, Thomas U. Mayer, Tarun M. Kapoor

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

A high-throughput, microscopy-based chemical-genetic screen identified HR22C16, which causes a monoastral mitotic block, as a small-molecule probe for cell division (see picture). By using a diastereoselective, traceless solid-phase synthesis and biological assays, a more potent HR22C16 analogue was then identified. A photocaging strategy for HR22C16 was also developed to allow fast temporal control over the function of the target protein Eg5.

Original languageEnglish (US)
Pages (from-to)2379-2382
Number of pages4
JournalAngewandte Chemie - International Edition
Volume42
Issue number21
DOIs
StatePublished - May 30 2003
Externally publishedYes

Fingerprint

Assays
Microscopic examination
Cells
Throughput
Proteins
Molecules
HR22C16

Keywords

  • Antimitotics
  • Inhibitors
  • Molecular motors
  • Photolysis
  • Solid-phase synthesis

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Hotha, S., Yarrow, J. C., Yang, J. G., Garrett, S. C., Renduchintala, K. V., Mayer, T. U., & Kapoor, T. M. (2003). HR22C16: A potent small-molecule probe for the dynamics of cell division. Angewandte Chemie - International Edition, 42(21), 2379-2382. https://doi.org/10.1002/anie.200351173

HR22C16 : A potent small-molecule probe for the dynamics of cell division. / Hotha, Srinivas; Yarrow, Justin C.; Yang, Janet G.; Garrett, Sarah C.; Renduchintala, Kishore V.; Mayer, Thomas U.; Kapoor, Tarun M.

In: Angewandte Chemie - International Edition, Vol. 42, No. 21, 30.05.2003, p. 2379-2382.

Research output: Contribution to journalArticle

Hotha, S, Yarrow, JC, Yang, JG, Garrett, SC, Renduchintala, KV, Mayer, TU & Kapoor, TM 2003, 'HR22C16: A potent small-molecule probe for the dynamics of cell division', Angewandte Chemie - International Edition, vol. 42, no. 21, pp. 2379-2382. https://doi.org/10.1002/anie.200351173
Hotha, Srinivas ; Yarrow, Justin C. ; Yang, Janet G. ; Garrett, Sarah C. ; Renduchintala, Kishore V. ; Mayer, Thomas U. ; Kapoor, Tarun M. / HR22C16 : A potent small-molecule probe for the dynamics of cell division. In: Angewandte Chemie - International Edition. 2003 ; Vol. 42, No. 21. pp. 2379-2382.
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