A high-throughput, microscopy-based chemical-genetic screen identified HR22C16, which causes a monoastral mitotic block, as a small-molecule probe for cell division (see picture). By using a diastereoselective, traceless solid-phase synthesis and biological assays, a more potent HR22C16 analogue was then identified. A photocaging strategy for HR22C16 was also developed to allow fast temporal control over the function of the target protein Eg5.
|Original language||English (US)|
|Number of pages||4|
|Journal||Angewandte Chemie - International Edition|
|State||Published - May 30 2003|
- Molecular motors
- Solid-phase synthesis
ASJC Scopus subject areas