HPV73 a nonvaccine type causes cervical cancer

Sergio M. Amaro-Filho, Ana Gradissimo, Mykhaylo Usyk, Fabio C.B. Moreira, Liz M. de Almeida, Miguel A.M. Moreira, Robert D. Burk

Research output: Contribution to journalArticle

Abstract

HPV73 is classified as possibly oncogenic. It is neither routinely evaluated in HPV screening, nor covered by any of the prophylactic vaccines. We sought to investigate the carcinogenic characteristics of HPV73. Molecular studies were performed on eight cervix cancer biopsy specimens containing HPV73 from a cross-sectional cancer cohort of 590 women referred to the National Cancer Institute in Rio de Janeiro, Brazil. Transcriptional activity of HPV73 was evaluated by detection of spliced transcripts of E6/E6* and E1^E4 in cDNA created from RNA isolated from fresh tissue. Disruption of viral E1 and E2 genes in the tumor DNA was assessed by overlapping PCR amplification. Evaluation of viral integration was performed using a customized capture panel and next-generation sequencing, and an in-house bioinformatic pipeline. HPV73 E6/E6* transcripts were found in 7/7 specimens with available RNA, and three also had HPV73 E1^E4 transcripts. Disruption of E1 and E2 genes was observed in 4/8 specimens. Integration of HPV73 sequences into the cancer cell genomes was identified in all cervix cancer tissues. These results provide evidence that HPV73 is an oncogenic virus that can cause invasive cervix cancer. With current molecular screening and HPV vaccination, not all cervix cancers will be prevented.

Original languageEnglish (US)
JournalInternational Journal of Cancer
DOIs
StatePublished - Jan 1 2019

Fingerprint

Uterine Cervical Neoplasms
RNA
Virus Integration
Neoplasms
Oncogenic Viruses
National Cancer Institute (U.S.)
Computational Biology
Genes
Brazil
Vaccination
Vaccines
Complementary DNA
Genome
Biopsy
Polymerase Chain Reaction
DNA

Keywords

  • cervical cancer
  • HPV
  • integration
  • oncogenic type
  • transcriptionally active

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Amaro-Filho, S. M., Gradissimo, A., Usyk, M., Moreira, F. C. B., de Almeida, L. M., Moreira, M. A. M., & Burk, R. D. (2019). HPV73 a nonvaccine type causes cervical cancer. International Journal of Cancer. https://doi.org/10.1002/ijc.32315

HPV73 a nonvaccine type causes cervical cancer. / Amaro-Filho, Sergio M.; Gradissimo, Ana; Usyk, Mykhaylo; Moreira, Fabio C.B.; de Almeida, Liz M.; Moreira, Miguel A.M.; Burk, Robert D.

In: International Journal of Cancer, 01.01.2019.

Research output: Contribution to journalArticle

Amaro-Filho, SM, Gradissimo, A, Usyk, M, Moreira, FCB, de Almeida, LM, Moreira, MAM & Burk, RD 2019, 'HPV73 a nonvaccine type causes cervical cancer', International Journal of Cancer. https://doi.org/10.1002/ijc.32315
Amaro-Filho SM, Gradissimo A, Usyk M, Moreira FCB, de Almeida LM, Moreira MAM et al. HPV73 a nonvaccine type causes cervical cancer. International Journal of Cancer. 2019 Jan 1. https://doi.org/10.1002/ijc.32315
Amaro-Filho, Sergio M. ; Gradissimo, Ana ; Usyk, Mykhaylo ; Moreira, Fabio C.B. ; de Almeida, Liz M. ; Moreira, Miguel A.M. ; Burk, Robert D. / HPV73 a nonvaccine type causes cervical cancer. In: International Journal of Cancer. 2019.
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