Hodgkin's cell lectin: An ectosialyltransferase and lymphocyte agglutinant related to the hepatic asialoglycoprotein receptor

Elisabeth M. Paietta, A. L. Hubbard, P. H. Wiernik, V. Diehl, R. J. Stockert

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Abstract

The galactophilic lectin expressed on the surface of cultured Hodgkin's cells, recently described by this laboratory, has binding characteristics similar to those of the hepatic asialoglycoprotein receptor (HBP), and has been recognized as a M(r) 55,000 (p55) membrane glycoprotein by a polyclonal antiserum to rat HBP. This study confirms the close structural relationship between the two lectins showing immunological cross-reactivity of monoclonal and polyclonal antibodies recognizing distinct epitopes on rat or human HBP. In support of the suggested dual nature of p55 as lectin and ectosialyltransferase, enzyme activity is inhibited by the monoclonal anti-HBP antibody, anti-HA 116. Cultured Hodgkin's cells, as purified HBP, agglutinate T-lymphocytes expressing hyposialylated membrane glycosyl determinants. This cell-cell interaction mediated by p55 results in the incorporation of sialic acid into lymphocyte surface asialo-glycans. The function of the Hodgkin's lectin as lymphocyte agglutinant in vitro suggests its role as an immunomodulator contributing to the immunodeficiencies associated with Hodgkin's disease.

Original languageEnglish (US)
Pages (from-to)2461-2467
Number of pages7
JournalCancer Research
Volume47
Issue number9
StatePublished - 1987

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Asialoglycoprotein Receptor
Sialyltransferases
Hodgkin Disease
Lectins
Lymphocytes
Cultured Cells
Monoclonal Antibodies
Membrane Glycoproteins
Immunologic Factors
N-Acetylneuraminic Acid
Cell Communication
Polysaccharides
Immune Sera
Epitopes
Anti-Idiotypic Antibodies
T-Lymphocytes
Membranes
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hodgkin's cell lectin : An ectosialyltransferase and lymphocyte agglutinant related to the hepatic asialoglycoprotein receptor. / Paietta, Elisabeth M.; Hubbard, A. L.; Wiernik, P. H.; Diehl, V.; Stockert, R. J.

In: Cancer Research, Vol. 47, No. 9, 1987, p. 2461-2467.

Research output: Contribution to journalArticle

Paietta, Elisabeth M. ; Hubbard, A. L. ; Wiernik, P. H. ; Diehl, V. ; Stockert, R. J. / Hodgkin's cell lectin : An ectosialyltransferase and lymphocyte agglutinant related to the hepatic asialoglycoprotein receptor. In: Cancer Research. 1987 ; Vol. 47, No. 9. pp. 2461-2467.
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