HMC-1 human mast cells synthesize neurotensin (NT) precursor, secrete bioactive NT-like peptide(s) and express NT receptor NTS1

David E. Cochrane, Robert E. Carraway, Kimberly Harrington, Melissa A. Laudano, Stephen Rawlings, Ross S. Feldberg

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective and design: To determine if mast cells synthesize the inflammatory peptide, neurotensin (NT), secrete immunoreactive and bioactive NT, and express the NT receptor NTS1. Materials: HMC-1 cells, pleural mast cells from Sprague-Dawley rats, LAD2 mast cells, and human cord blood mast cells were used. Treatment: HMC-1 cells were stimulated with NT, C48/80, mastoparan, or PGE 2. For changes in cutaneous vascular permeability, anesthetized rats were injected intravenously with Evans Blue dye and intradermally with saline, NT, histamine, diphenhydramine, and C48/80. Methods: RT-PCR was used to identify RNA transcripts. Histamine was measured by fluorometric assay. In vivo cutaneous vascular permeability assays, radio-immunoassays for NT, Western blotting for the NT precursor protein and NTS1 protein from HMC-1 cells and tissues from rats were used. Immunohistochemistry was used to identify NT precursor-like proteins in HMC-1 mast cells. Results: HMC-1 cells express mRNAs for NT precursor, PC5A processing enzyme and NTS1 receptor. Human cord blood mast cells and LAD2 mast cells express mRNA transcripts for NT precursor and NTS1. Western blotting showed NT precursor and NTS1 receptor in HMC1. Rat tissues with high numbers of mast cells contained NT precursor proteins. NT-like peptides from HMC-1 displayed NT-like bioactivity. Conclusions: HMC-1 mast cells synthesize and secrete immunoreactive and bioactive NT-like peptide(s) and express the NT receptor, suggesting that NT from mast cells might serve autocrine and paracrine roles.

Original languageEnglish (US)
Pages (from-to)1139-1151
Number of pages13
JournalInflammation Research
Volume60
Issue number12
DOIs
StatePublished - Dec 1 2011
Externally publishedYes

Fingerprint

Neurotensin Receptors
Neurotensin
Mast Cells
Peptides
Protein Precursors
Capillary Permeability
Fetal Blood
Histamine
Blood Cells
Western Blotting
Diphenhydramine
Evans Blue
Skin
RNA Precursors

Keywords

  • HMC-1 mast cell
  • Neurotensin
  • Neurotensin receptor
  • Tumor growth

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

HMC-1 human mast cells synthesize neurotensin (NT) precursor, secrete bioactive NT-like peptide(s) and express NT receptor NTS1. / Cochrane, David E.; Carraway, Robert E.; Harrington, Kimberly; Laudano, Melissa A.; Rawlings, Stephen; Feldberg, Ross S.

In: Inflammation Research, Vol. 60, No. 12, 01.12.2011, p. 1139-1151.

Research output: Contribution to journalArticle

Cochrane, David E. ; Carraway, Robert E. ; Harrington, Kimberly ; Laudano, Melissa A. ; Rawlings, Stephen ; Feldberg, Ross S. / HMC-1 human mast cells synthesize neurotensin (NT) precursor, secrete bioactive NT-like peptide(s) and express NT receptor NTS1. In: Inflammation Research. 2011 ; Vol. 60, No. 12. pp. 1139-1151.
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abstract = "Objective and design: To determine if mast cells synthesize the inflammatory peptide, neurotensin (NT), secrete immunoreactive and bioactive NT, and express the NT receptor NTS1. Materials: HMC-1 cells, pleural mast cells from Sprague-Dawley rats, LAD2 mast cells, and human cord blood mast cells were used. Treatment: HMC-1 cells were stimulated with NT, C48/80, mastoparan, or PGE 2. For changes in cutaneous vascular permeability, anesthetized rats were injected intravenously with Evans Blue dye and intradermally with saline, NT, histamine, diphenhydramine, and C48/80. Methods: RT-PCR was used to identify RNA transcripts. Histamine was measured by fluorometric assay. In vivo cutaneous vascular permeability assays, radio-immunoassays for NT, Western blotting for the NT precursor protein and NTS1 protein from HMC-1 cells and tissues from rats were used. Immunohistochemistry was used to identify NT precursor-like proteins in HMC-1 mast cells. Results: HMC-1 cells express mRNAs for NT precursor, PC5A processing enzyme and NTS1 receptor. Human cord blood mast cells and LAD2 mast cells express mRNA transcripts for NT precursor and NTS1. Western blotting showed NT precursor and NTS1 receptor in HMC1. Rat tissues with high numbers of mast cells contained NT precursor proteins. NT-like peptides from HMC-1 displayed NT-like bioactivity. Conclusions: HMC-1 mast cells synthesize and secrete immunoreactive and bioactive NT-like peptide(s) and express the NT receptor, suggesting that NT from mast cells might serve autocrine and paracrine roles.",
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AU - Cochrane, David E.

AU - Carraway, Robert E.

AU - Harrington, Kimberly

AU - Laudano, Melissa A.

AU - Rawlings, Stephen

AU - Feldberg, Ross S.

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AB - Objective and design: To determine if mast cells synthesize the inflammatory peptide, neurotensin (NT), secrete immunoreactive and bioactive NT, and express the NT receptor NTS1. Materials: HMC-1 cells, pleural mast cells from Sprague-Dawley rats, LAD2 mast cells, and human cord blood mast cells were used. Treatment: HMC-1 cells were stimulated with NT, C48/80, mastoparan, or PGE 2. For changes in cutaneous vascular permeability, anesthetized rats were injected intravenously with Evans Blue dye and intradermally with saline, NT, histamine, diphenhydramine, and C48/80. Methods: RT-PCR was used to identify RNA transcripts. Histamine was measured by fluorometric assay. In vivo cutaneous vascular permeability assays, radio-immunoassays for NT, Western blotting for the NT precursor protein and NTS1 protein from HMC-1 cells and tissues from rats were used. Immunohistochemistry was used to identify NT precursor-like proteins in HMC-1 mast cells. Results: HMC-1 cells express mRNAs for NT precursor, PC5A processing enzyme and NTS1 receptor. Human cord blood mast cells and LAD2 mast cells express mRNA transcripts for NT precursor and NTS1. Western blotting showed NT precursor and NTS1 receptor in HMC1. Rat tissues with high numbers of mast cells contained NT precursor proteins. NT-like peptides from HMC-1 displayed NT-like bioactivity. Conclusions: HMC-1 mast cells synthesize and secrete immunoreactive and bioactive NT-like peptide(s) and express the NT receptor, suggesting that NT from mast cells might serve autocrine and paracrine roles.

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