HIV-1 capsid stabilization assay

Thomas Fricke, Felipe Diaz-Griffero

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The stability of the HIV-1 core in the cytoplasm is crucial for productive HIV-1 infection. Mutations that stabilize or destabilize the core showed defects in HIV-1 reverse transcription and infection. We developed a novel and simple assay to measure stability of in vitro-assembled HIV-1 CA-NC complexes. This assay allowed us to demonstrate that cytosolic extracts strongly stabilize the HIV-1 core (Fricke et al., J Virol 87:10587-10597, 2013). By using our novel assay, one can measure the ability of different drugs to modulate the stability of in vitro-assembled HIV-1 CA-NC complexes, such as PF74, CAP-1, IXN-053, cyclosporine A, Bi2, and the peptide CAI. We also found that purified CPSF6 (1-321) protein stabilizes in vitro-assembled HIV-1 CA-NC complexes (Fricke et al., J Virol 87:10587-10597, 2013). Here we describe in detail the use of this capsid stability assay. We believe that our assay can be a powerful tool to assess HIV-1 capsid stability in vitro.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages39-47
Number of pages9
Volume1354
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1354
ISSN (Print)10643745

Keywords

  • Capsid
  • Core
  • HIV-1
  • Stability
  • Uncoating

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Fricke, T., & Diaz-Griffero, F. (2016). HIV-1 capsid stabilization assay. In Methods in Molecular Biology (Vol. 1354, pp. 39-47). (Methods in Molecular Biology; Vol. 1354). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3046-3_3