Abstract
The stability of the HIV-1 core in the cytoplasm is crucial for productive HIV-1 infection. Mutations that stabilize or destabilize the core showed defects in HIV-1 reverse transcription and infection. We developed a novel and simple assay to measure stability of in vitro-assembled HIV-1 CA-NC complexes. This assay allowed us to demonstrate that cytosolic extracts strongly stabilize the HIV-1 core (Fricke et al., J Virol 87:10587-10597, 2013). By using our novel assay, one can measure the ability of different drugs to modulate the stability of in vitro-assembled HIV-1 CA-NC complexes, such as PF74, CAP-1, IXN-053, cyclosporine A, Bi2, and the peptide CAI. We also found that purified CPSF6 (1-321) protein stabilizes in vitro-assembled HIV-1 CA-NC complexes (Fricke et al., J Virol 87:10587-10597, 2013). Here we describe in detail the use of this capsid stability assay. We believe that our assay can be a powerful tool to assess HIV-1 capsid stability in vitro.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 39-47 |
| Number of pages | 9 |
| Volume | 1354 |
| DOIs | |
| State | Published - 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1354 |
| ISSN (Print) | 10643745 |
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Keywords
- Capsid
- Core
- HIV-1
- Stability
- Uncoating
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
HIV-1 capsid stabilization assay. / Fricke, Thomas; Diaz-Griffero, Felipe.
Methods in Molecular Biology. Vol. 1354 Humana Press Inc., 2016. p. 39-47 (Methods in Molecular Biology; Vol. 1354).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - HIV-1 capsid stabilization assay
AU - Fricke, Thomas
AU - Diaz-Griffero, Felipe
PY - 2016
Y1 - 2016
N2 - The stability of the HIV-1 core in the cytoplasm is crucial for productive HIV-1 infection. Mutations that stabilize or destabilize the core showed defects in HIV-1 reverse transcription and infection. We developed a novel and simple assay to measure stability of in vitro-assembled HIV-1 CA-NC complexes. This assay allowed us to demonstrate that cytosolic extracts strongly stabilize the HIV-1 core (Fricke et al., J Virol 87:10587-10597, 2013). By using our novel assay, one can measure the ability of different drugs to modulate the stability of in vitro-assembled HIV-1 CA-NC complexes, such as PF74, CAP-1, IXN-053, cyclosporine A, Bi2, and the peptide CAI. We also found that purified CPSF6 (1-321) protein stabilizes in vitro-assembled HIV-1 CA-NC complexes (Fricke et al., J Virol 87:10587-10597, 2013). Here we describe in detail the use of this capsid stability assay. We believe that our assay can be a powerful tool to assess HIV-1 capsid stability in vitro.
AB - The stability of the HIV-1 core in the cytoplasm is crucial for productive HIV-1 infection. Mutations that stabilize or destabilize the core showed defects in HIV-1 reverse transcription and infection. We developed a novel and simple assay to measure stability of in vitro-assembled HIV-1 CA-NC complexes. This assay allowed us to demonstrate that cytosolic extracts strongly stabilize the HIV-1 core (Fricke et al., J Virol 87:10587-10597, 2013). By using our novel assay, one can measure the ability of different drugs to modulate the stability of in vitro-assembled HIV-1 CA-NC complexes, such as PF74, CAP-1, IXN-053, cyclosporine A, Bi2, and the peptide CAI. We also found that purified CPSF6 (1-321) protein stabilizes in vitro-assembled HIV-1 CA-NC complexes (Fricke et al., J Virol 87:10587-10597, 2013). Here we describe in detail the use of this capsid stability assay. We believe that our assay can be a powerful tool to assess HIV-1 capsid stability in vitro.
KW - Capsid
KW - Core
KW - HIV-1
KW - Stability
KW - Uncoating
UR - http://www.scopus.com/inward/record.url?scp=84952769524&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84952769524&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3046-3_3
DO - 10.1007/978-1-4939-3046-3_3
M3 - Chapter
C2 - 26714703
AN - SCOPUS:84952769524
VL - 1354
T3 - Methods in Molecular Biology
SP - 39
EP - 47
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -