Histoplasma capsulatum heat-shock 60 orchestrates the adaptation of the fungus to temperature stress

Allan Jefferson Guimarães, Ernesto S. Nakayasu, Tiago J P Sobreira, Radames J B Cordero, Leonardo Nimrichter, Igor C. Almeida, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Heat shock proteins (Hsps) are among the most widely distributed and evolutionary conserved proteins. Hsps are essential regulators of diverse constitutive metabolic processes and are markedly upregulated during stress. A 62 kDa Hsp (Hsp60) of Histoplasma capsulatum (Hc) is an immunodominant antigen and the major surface ligand to CR3 receptors on macrophages. However little is known about the function of this protein within the fungus. We characterized Hc Hsp60-protein interactions under different temperature to gain insights of its additional functions oncell wall dynamism, heat stress and pathogenesis. We conducted co-immunoprecipitations with antibodies to Hc Hsp60 using cytoplasmic and cell wall extracts. Interacting proteins were identified by shotgun proteomics. For the cell wall, 84 common interactions were identified among the 3 growth conditions, including proteins involved in heat-shock response, sugar and amino acid/protein metabolism and cell signaling. Unique interactions were found at each temperature [30°C (81 proteins), 37°C (14) and 37/40°C (47)]. There were fewer unique interactions in cytoplasm [30°C (6), 37°C (25) and 37/40°C (39)] and four common interactions, including additional Hsps and other known virulence factors. These results show the complexity of Hsp60 function and provide insights into Hc biology, which may lead to new avenues for the management of histoplasmosis.

Original languageEnglish (US)
Article numbere14660
JournalPLoS One
Volume6
Issue number2
DOIs
StatePublished - 2011

Fingerprint

Histoplasma capsulatum
Histoplasma
Fungi
heat stress
Shock
Hot Temperature
fungi
Temperature
Heat-Shock Proteins
heat shock proteins
temperature
Proteins
proteins
Cell Wall
Cells
cell walls
Macrophage-1 Antigen
Chaperonin 60
Cell signaling
histoplasmosis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Guimarães, A. J., Nakayasu, E. S., Sobreira, T. J. P., Cordero, R. J. B., Nimrichter, L., Almeida, I. C., & Nosanchuk, J. D. (2011). Histoplasma capsulatum heat-shock 60 orchestrates the adaptation of the fungus to temperature stress. PLoS One, 6(2), [e14660]. https://doi.org/10.1371/journal.pone.0014660

Histoplasma capsulatum heat-shock 60 orchestrates the adaptation of the fungus to temperature stress. / Guimarães, Allan Jefferson; Nakayasu, Ernesto S.; Sobreira, Tiago J P; Cordero, Radames J B; Nimrichter, Leonardo; Almeida, Igor C.; Nosanchuk, Joshua D.

In: PLoS One, Vol. 6, No. 2, e14660, 2011.

Research output: Contribution to journalArticle

Guimarães, AJ, Nakayasu, ES, Sobreira, TJP, Cordero, RJB, Nimrichter, L, Almeida, IC & Nosanchuk, JD 2011, 'Histoplasma capsulatum heat-shock 60 orchestrates the adaptation of the fungus to temperature stress', PLoS One, vol. 6, no. 2, e14660. https://doi.org/10.1371/journal.pone.0014660
Guimarães AJ, Nakayasu ES, Sobreira TJP, Cordero RJB, Nimrichter L, Almeida IC et al. Histoplasma capsulatum heat-shock 60 orchestrates the adaptation of the fungus to temperature stress. PLoS One. 2011;6(2). e14660. https://doi.org/10.1371/journal.pone.0014660
Guimarães, Allan Jefferson ; Nakayasu, Ernesto S. ; Sobreira, Tiago J P ; Cordero, Radames J B ; Nimrichter, Leonardo ; Almeida, Igor C. ; Nosanchuk, Joshua D. / Histoplasma capsulatum heat-shock 60 orchestrates the adaptation of the fungus to temperature stress. In: PLoS One. 2011 ; Vol. 6, No. 2.
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