Histopathologic and Immunologic Aspects of AlphaCor Artificial Corneal Failure

Marco Coassin, Cheng Zhang, W. Richard Green, James V. Aquavella, Esen K. Akpek

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: To describe the histopathologic and immunologic characteristics of late artificial corneal failure in a small series of patients who underwent AlphaCor implantation and to elucidate the mechanisms involved. Design: Clinicopathologic case series. Methods: Three patients were diagnosed with corneal melting during the late postoperative period and required the removal of the devices. The explanted devices embedded within the corneal tissues were examined by light microscopy, electron microscopy, and immunohistochemical studies. Results: Light microscopic examination of the specimens disclosed adequate biointegration with no foreign body response. Immunofluorescence studies of the skirt exhibited expression of inflammatory cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α), and some interferon γ (IFN-γ). The keratocytes stained positively for Thy-1 and smooth muscle actin but negatively for CD34. Conclusions: Although these findings confirm the occurrence of biointegration, myofibroblastic differentiation of the ingrowing keratocytes is a prominent feature.

Original languageEnglish (US)
JournalAmerican Journal of Ophthalmology
Volume144
Issue number5
DOIs
StatePublished - Nov 2007
Externally publishedYes

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Device Removal
Light
Foreign Bodies
Interleukin-1
Postoperative Period
Interferons
Freezing
Fluorescent Antibody Technique
Smooth Muscle
Actins
Microscopy
Electron Microscopy
Tumor Necrosis Factor-alpha
Cytokines
Equipment and Supplies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Histopathologic and Immunologic Aspects of AlphaCor Artificial Corneal Failure. / Coassin, Marco; Zhang, Cheng; Green, W. Richard; Aquavella, James V.; Akpek, Esen K.

In: American Journal of Ophthalmology, Vol. 144, No. 5, 11.2007.

Research output: Contribution to journalArticle

Coassin, Marco ; Zhang, Cheng ; Green, W. Richard ; Aquavella, James V. ; Akpek, Esen K. / Histopathologic and Immunologic Aspects of AlphaCor Artificial Corneal Failure. In: American Journal of Ophthalmology. 2007 ; Vol. 144, No. 5.
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