TY - JOUR
T1 - Histone gene expression during sea urchin spermatogenesis
T2 - An in situ hybridization study
AU - Poccia, Dominic
AU - Lieber, Toby
AU - Childs, Geoffrey
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1989
Y1 - 1989
N2 - The expression of testis‐specific and adult somatic histone genes in sea urchin testis was investigated by in situ hybridization. The testis‐specific histone genes (Sp H2B‐1 of Strongylocentrotus purpuratus and Sp H2B‐2 of Lytechinus pictus) were expressed exclusively in a subset of male germ line cells. These cells are morphologically identical to replicating cells pulse‐labeled with 3H‐thymidine. Genes coding for histones expressed in adult somatic and late embryo cells (H2A‐β for S. purpuratus and H3‐1 for L. Pictus) were expressed in the same germ line cells, as well as in the supportive cells (nutritive phagocytes) of the gonad. All histone mRNAs detected in the male germ lineage declined precipitously by the early spermatid stage, before cytoplasmic reduction. The data suggest that both testis‐specific and adult somatic histone genes are expressed in proliferating male germ line cells. Testis‐specific gene expression is restricted to spermatogonia and premeiotic spermatid, but somatic histone expression is not. The decline of histone mRNA in nondividing spermatids is not merely a consequence of cytoplasmic shedding, but probably reflects mRNA turnover.
AB - The expression of testis‐specific and adult somatic histone genes in sea urchin testis was investigated by in situ hybridization. The testis‐specific histone genes (Sp H2B‐1 of Strongylocentrotus purpuratus and Sp H2B‐2 of Lytechinus pictus) were expressed exclusively in a subset of male germ line cells. These cells are morphologically identical to replicating cells pulse‐labeled with 3H‐thymidine. Genes coding for histones expressed in adult somatic and late embryo cells (H2A‐β for S. purpuratus and H3‐1 for L. Pictus) were expressed in the same germ line cells, as well as in the supportive cells (nutritive phagocytes) of the gonad. All histone mRNAs detected in the male germ lineage declined precipitously by the early spermatid stage, before cytoplasmic reduction. The data suggest that both testis‐specific and adult somatic histone genes are expressed in proliferating male germ line cells. Testis‐specific gene expression is restricted to spermatogonia and premeiotic spermatid, but somatic histone expression is not. The decline of histone mRNA in nondividing spermatids is not merely a consequence of cytoplasmic shedding, but probably reflects mRNA turnover.
KW - Male germ line cells
KW - Nutritive phagocytes
KW - Testis
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U2 - 10.1002/mrd.1080010310
DO - 10.1002/mrd.1080010310
M3 - Article
C2 - 2627371
AN - SCOPUS:0024796617
SN - 1040-452X
VL - 1
SP - 219
EP - 229
JO - Molecular Reproduction and Development
JF - Molecular Reproduction and Development
IS - 3
ER -