Highly cooperative control of endocytosis by clathrin

Howard S. Moskowitz, Charles T. Yokoyama, Timothy A. Ryan

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Clathrin assembles into a dynamic two-dimensional lattice on the plasma membrane where it plays a critical role in endocytosis. To probe the regulation of this process, we used siRNA against clathrin, in combination with single cell assays for transferrin uptake as well as total internal reflection microscopy, to examine how endocytic rates and membrane dynamics depend upon cellular clathrin concentration ([Clathrin]). We find that endocytosis is tightly controlled by [Clathrin] over a very narrow dynamic range such that small changes in [Clathrin] can lead to large changes in endocytic rates, indicative of a highly cooperative process (apparent Hill coefficient, n > 6). The number of clathrin assemblies at the cell surface was invariant over a wide range of [Clathrin]; however, both the amount of clathrin in each assembly and the subsequent membrane dynamics were steeply dependent on [Clathrin]. Thus clathrin controls the structural dynamics of membrane internalization via a strongly cooperative process. We used this analysis to show that one important regulator of endocytosis, the actin cytoskeleton, acts noncompetitively as a modulator of clathrin function.

Original languageEnglish (US)
Pages (from-to)1769-1776
Number of pages8
JournalMolecular Biology of the Cell
Volume16
Issue number4
DOIs
StatePublished - Apr 2005
Externally publishedYes

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Clathrin
Endocytosis
Membranes
Transferrin
Actin Cytoskeleton
Small Interfering RNA
Microscopy
Cell Membrane

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Highly cooperative control of endocytosis by clathrin. / Moskowitz, Howard S.; Yokoyama, Charles T.; Ryan, Timothy A.

In: Molecular Biology of the Cell, Vol. 16, No. 4, 04.2005, p. 1769-1776.

Research output: Contribution to journalArticle

Moskowitz, Howard S. ; Yokoyama, Charles T. ; Ryan, Timothy A. / Highly cooperative control of endocytosis by clathrin. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 4. pp. 1769-1776.
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