High throughput fluorimetric assessment of iron traffic and chelation in iron-overloaded Caenorhabditis elegans

Breno Pannia Espósito, Airton Cunha Martins, Rodrigo Rodrigues Victor de Carvalho, Michael Aschner

Research output: Contribution to journalArticle

Abstract

The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.

Original languageEnglish (US)
Pages (from-to)255-267
Number of pages13
JournalBioMetals
Volume33
Issue number4-5
DOIs
StatePublished - Oct 1 2020

Keywords

  • Caenorhabditis elegans
  • Calcein
  • Chelator
  • Copper
  • Desferrioxamine
  • Fluorescence
  • Iron

ASJC Scopus subject areas

  • Biomaterials
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Metals and Alloys

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