Abstract
The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 255-267 |
| Number of pages | 13 |
| Journal | BioMetals |
| Volume | 33 |
| Issue number | 4-5 |
| DOIs | |
| State | Published - Oct 1 2020 |
Keywords
- Caenorhabditis elegans
- Calcein
- Chelator
- Copper
- Desferrioxamine
- Fluorescence
- Iron
ASJC Scopus subject areas
- Biomaterials
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
- Metals and Alloys
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Cite this
Espósito, B. P., Martins, A. C., de Carvalho, R. R. V., & Aschner, M. (2020). High throughput fluorimetric assessment of iron traffic and chelation in iron-overloaded Caenorhabditis elegans. BioMetals, 33(4-5), 255-267. https://doi.org/10.1007/s10534-020-00250-4