High throughput fluorimetric assessment of iron traffic and chelation in iron-overloaded Caenorhabditis elegans

Breno Pannia Espósito; Airton Cunha Martins; Rodrigo Rodrigues Victor de Carvalho; Michael Aschner

doi:10.1007/s10534-020-00250-4

High throughput fluorimetric assessment of iron traffic and chelation in iron-overloaded Caenorhabditis elegans

Breno Pannia Espósito, Airton Cunha Martins, Rodrigo Rodrigues Victor de Carvalho, Michael Aschner

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.

Original language	English (US)
Pages (from-to)	255-267
Number of pages	13
Journal	BioMetals
Volume	33
Issue number	4-5
DOIs	https://doi.org/10.1007/s10534-020-00250-4
State	Published - Oct 1 2020

Keywords

Caenorhabditis elegans
Calcein
Chelator
Copper
Desferrioxamine
Fluorescence
Iron

ASJC Scopus subject areas

Biomaterials
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
Metals and Alloys

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10.1007/s10534-020-00250-4

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abstract = "The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.",

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note = "Funding Information: The authors are grateful for the kind assistance of Dr. Tao Ke with the microscopy images. BPE acknowledges funding from the S{\~a}o Paulo Research Foundation (FAPESP 18/19684-0) and a travel grant from the Coordination of Superior Level Staff Improvement (CAPES 88887.371500/2019-00). MA was supported in part by grants from the National Institute of Environmental Health Sciences (NIEHS) R01ES10563 and R01ES07331. Publisher Copyright: {\textcopyright} 2020, Springer Nature B.V.",

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doi = "10.1007/s10534-020-00250-4",

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AU - Espósito, Breno Pannia

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AU - de Carvalho, Rodrigo Rodrigues Victor

AU - Aschner, Michael

N1 - Funding Information: The authors are grateful for the kind assistance of Dr. Tao Ke with the microscopy images. BPE acknowledges funding from the São Paulo Research Foundation (FAPESP 18/19684-0) and a travel grant from the Coordination of Superior Level Staff Improvement (CAPES 88887.371500/2019-00). MA was supported in part by grants from the National Institute of Environmental Health Sciences (NIEHS) R01ES10563 and R01ES07331. Publisher Copyright: © 2020, Springer Nature B.V.

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N2 - The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.

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KW - Caenorhabditis elegans

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KW - Copper

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KW - Fluorescence

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High throughput fluorimetric assessment of iron traffic and chelation in iron-overloaded Caenorhabditis elegans

Abstract

Keywords

ASJC Scopus subject areas

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