High throughput fluorimetric assessment of iron traffic and chelation in iron-overloaded Caenorhabditis elegans

Breno Pannia Espósito; Airton Cunha Martins; Rodrigo Rodrigues Victor de Carvalho; Michael Aschner

doi:10.1007/s10534-020-00250-4

High throughput fluorimetric assessment of iron traffic and chelation in iron-overloaded Caenorhabditis elegans

Breno Pannia Espósito, Airton Cunha Martins, Rodrigo Rodrigues Victor de Carvalho, Michael Aschner

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.

Original language	English (US)
Pages (from-to)	255-267
Number of pages	13
Journal	BioMetals
Volume	33
Issue number	4-5
DOIs	https://doi.org/10.1007/s10534-020-00250-4
State	Published - Oct 1 2020

Keywords

Caenorhabditis elegans
Calcein
Chelator
Copper
Desferrioxamine
Fluorescence
Iron

ASJC Scopus subject areas

Biomaterials
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
Metals and Alloys

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10534-020-00250-4

Cite this

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abstract = "The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.",

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AU - Espósito, Breno Pannia

AU - Martins, Airton Cunha

AU - de Carvalho, Rodrigo Rodrigues Victor

AU - Aschner, Michael

PY - 2020/10/1

Y1 - 2020/10/1

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AB - The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.

KW - Caenorhabditis elegans

KW - Calcein

KW - Chelator

KW - Copper

KW - Desferrioxamine

KW - Fluorescence

KW - Iron

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High throughput fluorimetric assessment of iron traffic and chelation in iron-overloaded Caenorhabditis elegans

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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