High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome

Jan A. Gossen, Wiljo J.F. de Leeuw, Aart Verwest, Paul H.M. Lohman, Jan Vijg

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of -5 - 10-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.

Original languageEnglish (US)
Pages (from-to)423-429
Number of pages7
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume250
Issue number1-2
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Keywords

  • LacZ shuttle vector
  • Mouse X-chromosome
  • Mutational 'hot spot'
  • Spontaneous mutations
  • Transgenic mice

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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