High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome

Jan A. Gossen, Wiljo J F de Leeuw, Aart Verwest, Paul H M Lohman, Jan Vijg

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of -5 - 10-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.

Original languageEnglish (US)
Pages (from-to)423-429
Number of pages7
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume250
Issue number1-2
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

X Chromosome
Mutation Rate
Transgenes
Transgenic Mice
Genome
Bacteriophage lambda
Genetic Vectors
Liver
Brain
Mutagenesis
DNA
Genes

Keywords

  • LacZ shuttle vector
  • Mouse X-chromosome
  • Mutational 'hot spot'
  • Spontaneous mutations
  • Transgenic mice

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis

Cite this

High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome. / Gossen, Jan A.; de Leeuw, Wiljo J F; Verwest, Aart; Lohman, Paul H M; Vijg, Jan.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 250, No. 1-2, 1991, p. 423-429.

Research output: Contribution to journalArticle

Gossen, Jan A. ; de Leeuw, Wiljo J F ; Verwest, Aart ; Lohman, Paul H M ; Vijg, Jan. / High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 1991 ; Vol. 250, No. 1-2. pp. 423-429.
@article{7c7a7828d1c040c28a94e6384336ecf9,
title = "High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome",
abstract = "To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of -5 - 10-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.",
keywords = "LacZ shuttle vector, Mouse X-chromosome, Mutational 'hot spot', Spontaneous mutations, Transgenic mice",
author = "Gossen, {Jan A.} and {de Leeuw}, {Wiljo J F} and Aart Verwest and Lohman, {Paul H M} and Jan Vijg",
year = "1991",
doi = "10.1016/0027-5107(91)90198-W",
language = "English (US)",
volume = "250",
pages = "423--429",
journal = "Mutation Research",
issn = "0027-5107",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - High somatic mutation frequencies in a LacZ transgene integrated on the mouse X-chromosome

AU - Gossen, Jan A.

AU - de Leeuw, Wiljo J F

AU - Verwest, Aart

AU - Lohman, Paul H M

AU - Vijg, Jan

PY - 1991

Y1 - 1991

N2 - To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of -5 - 10-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.

AB - To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of -5 - 10-6. Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.

KW - LacZ shuttle vector

KW - Mouse X-chromosome

KW - Mutational 'hot spot'

KW - Spontaneous mutations

KW - Transgenic mice

UR - http://www.scopus.com/inward/record.url?scp=0025873390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025873390&partnerID=8YFLogxK

U2 - 10.1016/0027-5107(91)90198-W

DO - 10.1016/0027-5107(91)90198-W

M3 - Article

VL - 250

SP - 423

EP - 429

JO - Mutation Research

JF - Mutation Research

SN - 0027-5107

IS - 1-2

ER -