High-Frequency Dynamic Nuclear Polarization in the Nuclear Rotating Frame

C. T. Farrar, D. A. Hall, Gary J. Gerfen, M. Rosay, J. H. Ardenkjær-Larsen, R. G. Griffin

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A proton dynamic nuclear polarization (DNP) NMR signal enhancement (e) close to thermal equilibrium, ∈ = 0.89, has been obtained at high field (B0 = 5 T, νepr = 139.5 GHz) using 15 mM trityl radical in a 40:60 water/glycerol frozen solution at 11 K. The electron-nuclear polarization transfer is performed in the nuclear rotating frame with microwave irradiation during a nuclear spin-lock pulse. The growth of the signal enhancement is governed by the rotating frame nuclear spin-lattice relaxation time (T1p), which is four orders of magnitude shorter than the nuclear spin-lattice relaxation time (T1n). Due to the rapid polarization transfer in the nuclear rotating frame the experiment can be recycled at a rate of 1/T1p and is not limited by the much slower lab frame nuclear spin-lattice relaxation rate (1/T1n). The increased repetition rate allowed in the nuclear rotating frame provides an effective enhancement per unit time1/2 of ∈t = 197. The nuclear rotating frame-DNP experiment does not require high microwave power; significant signal enhancements were obtained with a low-power (20 mW) Gunn diode microwave source and no microwave resonant structure. The symmetric trityl radical used as the polarization source is water-soluble and has a narrow EPR linewidth of 10 G at 139.5 GHz making it an ideal polarization source for high-field DNP/NMR studies of biological systems,

Original languageEnglish (US)
Pages (from-to)134-141
Number of pages8
JournalJournal of Magnetic Resonance
Volume144
Issue number1
DOIs
StatePublished - May 2000

Fingerprint

Microwaves
Polarization
nuclear spin
polarization
Spin-lattice relaxation
spin-lattice relaxation
microwaves
augmentation
Water
Relaxation time
Glycerol
Protons
relaxation time
Nuclear magnetic resonance
Gunn diodes
Hot Temperature
Electrons
nuclear magnetic resonance
Microwave irradiation
Biological systems

ASJC Scopus subject areas

  • Molecular Biology
  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

Farrar, C. T., Hall, D. A., Gerfen, G. J., Rosay, M., Ardenkjær-Larsen, J. H., & Griffin, R. G. (2000). High-Frequency Dynamic Nuclear Polarization in the Nuclear Rotating Frame. Journal of Magnetic Resonance, 144(1), 134-141. https://doi.org/10.1006/jmre.2000.2025

High-Frequency Dynamic Nuclear Polarization in the Nuclear Rotating Frame. / Farrar, C. T.; Hall, D. A.; Gerfen, Gary J.; Rosay, M.; Ardenkjær-Larsen, J. H.; Griffin, R. G.

In: Journal of Magnetic Resonance, Vol. 144, No. 1, 05.2000, p. 134-141.

Research output: Contribution to journalArticle

Farrar, CT, Hall, DA, Gerfen, GJ, Rosay, M, Ardenkjær-Larsen, JH & Griffin, RG 2000, 'High-Frequency Dynamic Nuclear Polarization in the Nuclear Rotating Frame', Journal of Magnetic Resonance, vol. 144, no. 1, pp. 134-141. https://doi.org/10.1006/jmre.2000.2025
Farrar, C. T. ; Hall, D. A. ; Gerfen, Gary J. ; Rosay, M. ; Ardenkjær-Larsen, J. H. ; Griffin, R. G. / High-Frequency Dynamic Nuclear Polarization in the Nuclear Rotating Frame. In: Journal of Magnetic Resonance. 2000 ; Vol. 144, No. 1. pp. 134-141.
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