Hexanedione effects on protein phosphorylation in rat peripheral nerve

Kelly L. Horan, Joseph Eichberg, Liliana N. Berti-Mattera, Richard M. LoPachin

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Rats were treated with either 2,5-hexanedione (2,5-HD), 1,6-hexanediol (1,6-HDIOL), or saline for 7, 15 or 24 days. Protein phosphorylation was measured in proximal and distal sciatic nerve segments following incubation with [32P]orthophosphate. In proximal segments, 2,5-HD administration caused selective time-dependent increases in isotope incorporation in a 55 kDa protein, tentatively identified as tubulin, and a 180 kDa protein. Enhanced phosphorylation was highest at 24 days when motor function was most impaired. Administration of 1,6-HDIOL produced no consistent phosphorylation changes. Animals intoxicated with 3,4-dimethyl-2,5-hexanedione for 12 days showed proximal region increases in phosphorylation of the 55 and 180 kDa proteins and the major myelin proteins, Po and Pr.

Original languageEnglish (US)
Pages (from-to)366-370
Number of pages5
JournalBrain Research
Volume491
Issue number2
DOIs
StatePublished - Jul 10 1989
Externally publishedYes

Keywords

  • Axonopathy
  • Hexanedione
  • Nerve injury
  • Neurotoxicity
  • Protein phosphorylation
  • Sciatic nerve

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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