TY - JOUR
T1 - Hexanedione effects on protein phosphorylation in rat peripheral nerve
AU - Horan, Kelly L.
AU - Eichberg, Joseph
AU - Berti-Mattera, Liliana N.
AU - LoPachin, Richard M.
N1 - Funding Information:
The authorsw ish to acknowledgeth e technical expertisea ndhelp of Drs. David Caprettea ndJoan Lowery. DMHD was kindly providedb y Dr. Doyle Graham, Duke University Medical School. This work was supportedb y NIH Grants ES 03830( to R.M.L.) and DK 30577( to J.E.).
PY - 1989/7/10
Y1 - 1989/7/10
N2 - Rats were treated with either 2,5-hexanedione (2,5-HD), 1,6-hexanediol (1,6-HDIOL), or saline for 7, 15 or 24 days. Protein phosphorylation was measured in proximal and distal sciatic nerve segments following incubation with [32P]orthophosphate. In proximal segments, 2,5-HD administration caused selective time-dependent increases in isotope incorporation in a 55 kDa protein, tentatively identified as tubulin, and a 180 kDa protein. Enhanced phosphorylation was highest at 24 days when motor function was most impaired. Administration of 1,6-HDIOL produced no consistent phosphorylation changes. Animals intoxicated with 3,4-dimethyl-2,5-hexanedione for 12 days showed proximal region increases in phosphorylation of the 55 and 180 kDa proteins and the major myelin proteins, Po and Pr.
AB - Rats were treated with either 2,5-hexanedione (2,5-HD), 1,6-hexanediol (1,6-HDIOL), or saline for 7, 15 or 24 days. Protein phosphorylation was measured in proximal and distal sciatic nerve segments following incubation with [32P]orthophosphate. In proximal segments, 2,5-HD administration caused selective time-dependent increases in isotope incorporation in a 55 kDa protein, tentatively identified as tubulin, and a 180 kDa protein. Enhanced phosphorylation was highest at 24 days when motor function was most impaired. Administration of 1,6-HDIOL produced no consistent phosphorylation changes. Animals intoxicated with 3,4-dimethyl-2,5-hexanedione for 12 days showed proximal region increases in phosphorylation of the 55 and 180 kDa proteins and the major myelin proteins, Po and Pr.
KW - Axonopathy
KW - Hexanedione
KW - Nerve injury
KW - Neurotoxicity
KW - Protein phosphorylation
KW - Sciatic nerve
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U2 - 10.1016/0006-8993(89)90072-3
DO - 10.1016/0006-8993(89)90072-3
M3 - Article
C2 - 2765891
AN - SCOPUS:0024341559
SN - 0006-8993
VL - 491
SP - 366
EP - 370
JO - Brain research
JF - Brain research
IS - 2
ER -