Abstract
Accumulating evidence has shown that immunoglobulin (Ig) is unexpectedly expressed by epithelial cancer cells and that it can promote tumor growth. The main purpose of this study was to explore the components of the cancerous Ig and its possible function. The presence of cancerous Ig in the Golgi apparatus was confirmed by immunofluorescence, indirectly suggesting that the cancerous Ig was processed and packaged in cancer cells. Western blot analysis and ELISA results indicated that cancer cells produced membrane Ig and secreted Ig into the supernatant fraction. The cancerous Ig consists of an Î ± heavy chain and a ΰlight chain. Finally, by analyzing the Ig components pulled down by protein A beads, the cancerous Ig was found to be structurally distinct from normal Ig. The cancerous Ig was truncated or aberrant. Although the underlying mechanism that causes the abnormalities has not been determined, our current discoveries strengthen our previous findings and promise fruitful future explorations.
Original language | English (US) |
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Pages (from-to) | 479-485 |
Number of pages | 7 |
Journal | Cellular and Molecular Immunology |
Volume | 8 |
Issue number | 6 |
DOIs | |
State | Published - Nov 2011 |
Externally published | Yes |
Keywords
- immunoglobulin
- truncated immunoglobulin
- tumor immunology
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Infectious Diseases