Heterogeneity of aberrant immunoglobulin expression in cancer cells

Duosha Hu, Zhi Duan, Ming Li, Yiqun Jiang, Haidan Liu, Hui Zheng, Lili Li, Ann M. Bode, Zigang Dong, Ya Cao

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Accumulating evidence has shown that immunoglobulin (Ig) is unexpectedly expressed by epithelial cancer cells and that it can promote tumor growth. The main purpose of this study was to explore the components of the cancerous Ig and its possible function. The presence of cancerous Ig in the Golgi apparatus was confirmed by immunofluorescence, indirectly suggesting that the cancerous Ig was processed and packaged in cancer cells. Western blot analysis and ELISA results indicated that cancer cells produced membrane Ig and secreted Ig into the supernatant fraction. The cancerous Ig consists of an Î ± heavy chain and a ΰlight chain. Finally, by analyzing the Ig components pulled down by protein A beads, the cancerous Ig was found to be structurally distinct from normal Ig. The cancerous Ig was truncated or aberrant. Although the underlying mechanism that causes the abnormalities has not been determined, our current discoveries strengthen our previous findings and promise fruitful future explorations.

Original languageEnglish (US)
Pages (from-to)479-485
Number of pages7
JournalCellular and Molecular Immunology
Volume8
Issue number6
DOIs
StatePublished - Nov 2011

Keywords

  • immunoglobulin
  • truncated immunoglobulin
  • tumor immunology

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Heterogeneity of aberrant immunoglobulin expression in cancer cells'. Together they form a unique fingerprint.

Cite this