Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice

Daniel Benten, Antonia Follenzi, Kuldeep K. Bhargava, Vinay Kumaran, Christopher J. Palestro, Sanjeev Gupta

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Targeting of cells to specific tissues is critical for cell therapy. To study endothelial cell targeting, we isolated mouse liver sinusoidal endothelial cells (LSEC) and examined cell biodistributions in animals. To identify transplanted LSEC in tissues, we labeled cells metabolically with DiI-conjugated acetylated low density lipoprotein particles (DiI-Ac-LDL) or 111Indium-oxine, used LSEC from Rosa26 donors expressing β-galactosidase or Tie-2-GFP donors with green fluorescent protein (GFP) expression, and tranduced LSEC with a GFP-lentiviral vector. LSEC efficiently incorporated 111Indium and DiI-Ac-LDL and expressed GFP introduced by the lentiviral vector. Use of radiolabeled LSEC showed differences in cell biodistributions in relation to the cell transplantation route. After intraportal injection, LSEC were largely in the liver (60 ± 13%) and, after systemic intravenous injection, in lungs (67 ± 9%); however, after intrasplenic injection, only some LSEC remained in the spleen (29 ± 10%; P < .01), whereas most LSEC migrated to the liver or lungs. Transplanted LSEC were found in the liver, lungs, and spleen shortly after transplantation, whereas longer-term cell survival was observed only in the liver. Transplanted LSEC were distinct from Kupffer cells with expression of Tie-2 promoter-driven GFP and of CD31, without F4/80 reactivity. In further studies using radiolabeled LSEC, we established that the manipulation of receptor-mediated cell adhesion in liver sinusoids or the manipulation of blood flow-dependent cell exit from sinusoids improved intrahepatic retention of LSEC to 89 ± 7% and 89 ± 5%, respectively (P < .01). In conclusion, the targeting of LSEC to the liver and other organs is directed by vascular bed-specific mechanisms, including blood flow-related processes, and cell-specific factors. These findings may facilitate analysis of LSEC for cell and gene therapy applications.

Original languageEnglish (US)
Pages (from-to)140-148
Number of pages9
JournalHepatology
Volume42
Issue number1
DOIs
StatePublished - Jul 1 2005

ASJC Scopus subject areas

  • Hepatology

Fingerprint Dive into the research topics of 'Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice'. Together they form a unique fingerprint.

  • Cite this

    Benten, D., Follenzi, A., Bhargava, K. K., Kumaran, V., Palestro, C. J., & Gupta, S. (2005). Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice. Hepatology, 42(1), 140-148. https://doi.org/10.1002/hep.20746