Hemodialysis-induced changes in the blood composition affect function of the endothelium

Weronika Oleśkowska-Florek, Alicja Polubinska, Ewa Baum, Monika Matecka, Malgorzata Pyda, Krzysztof Pawlaczyk, Andrzej Breborowicz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hemodialysis induces oxidative stress causing intravascular inflammation, which may cause endothelial dysfunction. We evaluated how hemodialysis-induced changes in blood affect the function of endothelial cells in in vitro culture. Serum samples were collected from 42 uremic patients treated with hemodialysis, one before the start of dialysis and the other one at the end of session. All patients were dialysed with polysulfone dialyzer. Concentrations of the inflammatory molecules carbonyl protein and metabolites of NO synthesis were measured in blood. Additionally, the effect of the serum obtained before and after dialysis on the function of endothelial cells in in vitro culture was studied. Hemodialysis caused increase of monocyte chemoattractant protein (MCP)-1 (+17%), hepatocyte growth factor (+91%), and pentraxin-3 (+30%) concentration in serum. Concentration of carbonyl protein was decreased by 30%. Decrease of blood level of asymmetric dimethylarginine (-25%) and nitrate/nitrites (-62%) was observed. Serum obtained after hemodialysis stimulated proliferation of endothelial cells (+10%) and synthesis of MCP-1(+11%) in these cells. Hemodialysis-induced intravascular inflammation changes the function of endothelial cells, which may lead to acceleration of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)650-656
Number of pages7
JournalHemodialysis International
Volume18
Issue number3
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Endothelium
Renal Dialysis
Endothelial Cells
Chemokine CCL2
Serum
Dialysis
Inflammation
Hepatocyte Growth Factor
Nitrites
Nitrates
Atherosclerosis
Proteins
Oxidative Stress
In Vitro Techniques

Keywords

  • Endothelium
  • Hemodialysis
  • Inflammation

ASJC Scopus subject areas

  • Hematology
  • Nephrology
  • Medicine(all)

Cite this

Oleśkowska-Florek, W., Polubinska, A., Baum, E., Matecka, M., Pyda, M., Pawlaczyk, K., & Breborowicz, A. (2014). Hemodialysis-induced changes in the blood composition affect function of the endothelium. Hemodialysis International, 18(3), 650-656. https://doi.org/10.1111/hdi.12148

Hemodialysis-induced changes in the blood composition affect function of the endothelium. / Oleśkowska-Florek, Weronika; Polubinska, Alicja; Baum, Ewa; Matecka, Monika; Pyda, Malgorzata; Pawlaczyk, Krzysztof; Breborowicz, Andrzej.

In: Hemodialysis International, Vol. 18, No. 3, 2014, p. 650-656.

Research output: Contribution to journalArticle

Oleśkowska-Florek, W, Polubinska, A, Baum, E, Matecka, M, Pyda, M, Pawlaczyk, K & Breborowicz, A 2014, 'Hemodialysis-induced changes in the blood composition affect function of the endothelium', Hemodialysis International, vol. 18, no. 3, pp. 650-656. https://doi.org/10.1111/hdi.12148
Oleśkowska-Florek W, Polubinska A, Baum E, Matecka M, Pyda M, Pawlaczyk K et al. Hemodialysis-induced changes in the blood composition affect function of the endothelium. Hemodialysis International. 2014;18(3):650-656. https://doi.org/10.1111/hdi.12148
Oleśkowska-Florek, Weronika ; Polubinska, Alicja ; Baum, Ewa ; Matecka, Monika ; Pyda, Malgorzata ; Pawlaczyk, Krzysztof ; Breborowicz, Andrzej. / Hemodialysis-induced changes in the blood composition affect function of the endothelium. In: Hemodialysis International. 2014 ; Vol. 18, No. 3. pp. 650-656.
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