Heme-induced neutrophil extracellular traps contribute to the pathogenesis of sickle cell disease

Grace Chen, Dachuan Zhang, Tobias A. Fuchs, Deepa G. Manwani, Denisa D. Wagner, Paul S. Frenette

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Sickle cell disease (SCD) is characterized by recurring episodes of vascular occlusion in which neutrophil activation plays a major role. The disease is associated with chronic hemolysis with elevated cell-free hemoglobin and heme. The ensuing depletion of heme scavenger proteins leads to nonspecific heme uptake and heme-catalyzed generation of reactive oxygen species. Here, we have identified a novel role for heme in the induction of neutrophil extracellular trap (NET) formation in SCD. NETs are decondensed chromatin decorated by granular enzymes and are released by activated neutrophils. In humanized SCD mice, we have detected NETs in the lungs and soluble NET components in plasma. The presence of NETs was associated with hypothermia and death of these mice, which could be prevented and delayed, respectively, by dismantling NETs with DNase I treatment. We have identified heme as the plasma factor that stimulates neutrophils to release NETs in vitro and in vivo. Increasing or decreasing plasma heme concentrations can induce or prevent, respectively, in vivo NET formation, indicating that heme plays a crucial role in stimulating NET release in SCD. Our results thus suggest that NETs significantly contribute to SCD pathogenesis and can serve as a therapeutic target for treating SCD.

Original languageEnglish (US)
Pages (from-to)3818-3827
Number of pages10
JournalBlood
Volume123
Issue number24
DOIs
StatePublished - Jun 12 2014

Fingerprint

Sickle Cell Anemia
Heme
Plasmas
Neutrophils
Hypothermia
Neutrophil Activation
Extracellular Traps
Deoxyribonuclease I
Hemolysis
Chromatin
Blood Vessels
Reactive Oxygen Species
Hemoglobins
Chemical activation
Lung
Enzymes

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Heme-induced neutrophil extracellular traps contribute to the pathogenesis of sickle cell disease. / Chen, Grace; Zhang, Dachuan; Fuchs, Tobias A.; Manwani, Deepa G.; Wagner, Denisa D.; Frenette, Paul S.

In: Blood, Vol. 123, No. 24, 12.06.2014, p. 3818-3827.

Research output: Contribution to journalArticle

Chen, Grace ; Zhang, Dachuan ; Fuchs, Tobias A. ; Manwani, Deepa G. ; Wagner, Denisa D. ; Frenette, Paul S. / Heme-induced neutrophil extracellular traps contribute to the pathogenesis of sickle cell disease. In: Blood. 2014 ; Vol. 123, No. 24. pp. 3818-3827.
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