Helicobacter pylori strain types and risk of gastric cancer: A case-control study

H. Enroth, W. Kraaz, L. Engstrand, O. Nyren, Thomas E. Rohan

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The aim of this novel endoscopy clinic-based case-control study was to explore the influence of different Helicobacter pylori strain types on the risk of gastric adenocarcinoma using isolated bacterial strains, tissue samples, and sera. We included 72 cases with gastric adenocarcinoma and 324 age- and sex-matched controls. Histological characterization, culture, molecular typing of H. pylori genes by PCR (cagA/vacA), conventional IgG ELISA, and immunoblotting (Western blot) for the CagA and VacA proteins were performed. With four tests combined, H. pylori infection was detected in 57 (79%) cases and 213 (66%) controls. A positive association between H. pylori infection and gastric cancer risk was found [odds ratio (OR), 2.1; 95% confidence interval, 1.1-3.9]. Type I (OR, 1.8), intermediate (OR, 2.0), and type II (OR, 0.2) strains of H. pylori presented different serum antibody levels and different levels of association with gastric cancer. Our case-control study, based on molecular characterization and serology, provides further evidence that infection by more virulent strains of H. pylori and the presence of antibodies toward the CagA protein can be used as markers for an increased risk of gastric adenocarcinoma and that the strain types of H. pylori could be used in the future to determine disease outcome.

Original languageEnglish (US)
Pages (from-to)981-985
Number of pages5
JournalCancer Epidemiology Biomarkers and Prevention
Volume9
Issue number9
StatePublished - 2000

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Helicobacter pylori
Stomach Neoplasms
Case-Control Studies
Odds Ratio
Stomach
Adenocarcinoma
Helicobacter Infections
Molecular Typing
Antibodies
Serology
Serum
Immunoblotting
Endoscopy
Proteins
Immunoglobulin G
Western Blotting
Enzyme-Linked Immunosorbent Assay
Confidence Intervals
Polymerase Chain Reaction
Infection

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Helicobacter pylori strain types and risk of gastric cancer : A case-control study. / Enroth, H.; Kraaz, W.; Engstrand, L.; Nyren, O.; Rohan, Thomas E.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 9, No. 9, 2000, p. 981-985.

Research output: Contribution to journalArticle

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