Heat stress and hydrocortisone are independent stimulators of triiodothyronine-induced growth hormone production in cultured rat somatotrophic tumour cells

L. E. Shapiro, C. P. Katz, S. H S Wasserman, C. R. DeFesi, Martin I. Surks

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have reported that, in cultured GC cells, the stress of incubation at 41°C enhances thyroid hormone stimulation of growth hormone (GH) in a manner similar to the effects observed in a model of nonthyroidal disease in rats. Since glucocorticoids are potentially involved in stress responses both in vivo and in cell culture, we studied the role of glucocorticoid in the enhancement of (which are rat somatotrophic tumor cells) triiodothyronine (T3)-induced GH synthesis due to heat stress. Hydrocortisone addition increased T3-induced GH synthesis and GH mRNA content in cultured GC cells at both 37Γ and 41Γ. Depletion of glucocorticoid endogenous to serum supplement of the tissue culture medium did not prevent the enhancement of T3-induced GH synthesis that occurred during incubation at 41Γ. The levels and affinity of glucocorticoid cytosolic receptors were not enhanced during incubation at 41Γ. Lastly, no change in the sedimentation coefficient of the cytosolic glucocorticoid receptor or in its translocation into the nucleus occurred during incubation at 41Γ. Thus, the enhancement of T3-induced GH production in GC cells by heat stress appeared independent of the effect of glucocorticoids and not mediated through glucocorticoid receptors.

Original languageEnglish (US)
Pages (from-to)417-424
Number of pages8
JournalActa Endocrinologica
Volume124
Issue number4
StatePublished - 1991

Fingerprint

Triiodothyronine
Growth Hormone
Hydrocortisone
Hot Temperature
Glucocorticoids
Glucocorticoid Receptors
Neoplasms
Cultured Cells
Thyroid Hormones
Culture Media
Cell Culture Techniques
Messenger RNA
Serum

ASJC Scopus subject areas

  • Endocrinology

Cite this

Heat stress and hydrocortisone are independent stimulators of triiodothyronine-induced growth hormone production in cultured rat somatotrophic tumour cells. / Shapiro, L. E.; Katz, C. P.; Wasserman, S. H S; DeFesi, C. R.; Surks, Martin I.

In: Acta Endocrinologica, Vol. 124, No. 4, 1991, p. 417-424.

Research output: Contribution to journalArticle

@article{ee5773c6510f4c6fa18a165fa2f624d9,
title = "Heat stress and hydrocortisone are independent stimulators of triiodothyronine-induced growth hormone production in cultured rat somatotrophic tumour cells",
abstract = "We have reported that, in cultured GC cells, the stress of incubation at 41°C enhances thyroid hormone stimulation of growth hormone (GH) in a manner similar to the effects observed in a model of nonthyroidal disease in rats. Since glucocorticoids are potentially involved in stress responses both in vivo and in cell culture, we studied the role of glucocorticoid in the enhancement of (which are rat somatotrophic tumor cells) triiodothyronine (T3)-induced GH synthesis due to heat stress. Hydrocortisone addition increased T3-induced GH synthesis and GH mRNA content in cultured GC cells at both 37Γ and 41Γ. Depletion of glucocorticoid endogenous to serum supplement of the tissue culture medium did not prevent the enhancement of T3-induced GH synthesis that occurred during incubation at 41Γ. The levels and affinity of glucocorticoid cytosolic receptors were not enhanced during incubation at 41Γ. Lastly, no change in the sedimentation coefficient of the cytosolic glucocorticoid receptor or in its translocation into the nucleus occurred during incubation at 41Γ. Thus, the enhancement of T3-induced GH production in GC cells by heat stress appeared independent of the effect of glucocorticoids and not mediated through glucocorticoid receptors.",
author = "Shapiro, {L. E.} and Katz, {C. P.} and Wasserman, {S. H S} and DeFesi, {C. R.} and Surks, {Martin I.}",
year = "1991",
language = "English (US)",
volume = "124",
pages = "417--424",
journal = "European Journal of Endocrinology",
issn = "0804-4643",
publisher = "BioScientifica Ltd.",
number = "4",

}

TY - JOUR

T1 - Heat stress and hydrocortisone are independent stimulators of triiodothyronine-induced growth hormone production in cultured rat somatotrophic tumour cells

AU - Shapiro, L. E.

AU - Katz, C. P.

AU - Wasserman, S. H S

AU - DeFesi, C. R.

AU - Surks, Martin I.

PY - 1991

Y1 - 1991

N2 - We have reported that, in cultured GC cells, the stress of incubation at 41°C enhances thyroid hormone stimulation of growth hormone (GH) in a manner similar to the effects observed in a model of nonthyroidal disease in rats. Since glucocorticoids are potentially involved in stress responses both in vivo and in cell culture, we studied the role of glucocorticoid in the enhancement of (which are rat somatotrophic tumor cells) triiodothyronine (T3)-induced GH synthesis due to heat stress. Hydrocortisone addition increased T3-induced GH synthesis and GH mRNA content in cultured GC cells at both 37Γ and 41Γ. Depletion of glucocorticoid endogenous to serum supplement of the tissue culture medium did not prevent the enhancement of T3-induced GH synthesis that occurred during incubation at 41Γ. The levels and affinity of glucocorticoid cytosolic receptors were not enhanced during incubation at 41Γ. Lastly, no change in the sedimentation coefficient of the cytosolic glucocorticoid receptor or in its translocation into the nucleus occurred during incubation at 41Γ. Thus, the enhancement of T3-induced GH production in GC cells by heat stress appeared independent of the effect of glucocorticoids and not mediated through glucocorticoid receptors.

AB - We have reported that, in cultured GC cells, the stress of incubation at 41°C enhances thyroid hormone stimulation of growth hormone (GH) in a manner similar to the effects observed in a model of nonthyroidal disease in rats. Since glucocorticoids are potentially involved in stress responses both in vivo and in cell culture, we studied the role of glucocorticoid in the enhancement of (which are rat somatotrophic tumor cells) triiodothyronine (T3)-induced GH synthesis due to heat stress. Hydrocortisone addition increased T3-induced GH synthesis and GH mRNA content in cultured GC cells at both 37Γ and 41Γ. Depletion of glucocorticoid endogenous to serum supplement of the tissue culture medium did not prevent the enhancement of T3-induced GH synthesis that occurred during incubation at 41Γ. The levels and affinity of glucocorticoid cytosolic receptors were not enhanced during incubation at 41Γ. Lastly, no change in the sedimentation coefficient of the cytosolic glucocorticoid receptor or in its translocation into the nucleus occurred during incubation at 41Γ. Thus, the enhancement of T3-induced GH production in GC cells by heat stress appeared independent of the effect of glucocorticoids and not mediated through glucocorticoid receptors.

UR - http://www.scopus.com/inward/record.url?scp=0025730993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025730993&partnerID=8YFLogxK

M3 - Article

C2 - 2031440

AN - SCOPUS:0025730993

VL - 124

SP - 417

EP - 424

JO - European Journal of Endocrinology

JF - European Journal of Endocrinology

SN - 0804-4643

IS - 4

ER -