Heat shock protein 90-eNOS interactions mature with postnatal age in the pulmonary circulation of the piglet

Judy L. Aschner, Heng Zeng, Mark R. Kaplowitz, Yongmei Zhang, James C. Slaughter, Candice D. Fike

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Binding of endothelial nitric oxide synthase (eNOS) to the chaperone protein, Hsp90, promotes coupled eNOS synthetic activity. Using resistance level pulmonary arteries (PRA) from 2-day-, 5- to 7-day-, and 12-day-old piglets, we tested the hypothesis that Hsp90-eNOS interactions are developmentally regulated in the early neonatal period. PRA were isolated for coimmunoprecipitation and immunoblot analyses or cannulated for continuous diameter measurements using the pressurized myography technique. NOS inhibition caused less constriction in PRA from 2-day- compared with 5- to 7-day- and 12-day-old piglets. No age-related differences were found in dilation responses to an NO donor or in protein expression of Hsp90, phospho-eNOS (Ser1177), Akt, phospho-Akt, or caveolin-1. Compared with the older animals, PRA from 2-day-old piglets had higher total eNOS expression but displayed less binding of eNOS to Hsp90 and Akt. Hsp90 antagonism with radicicol induced greatest constriction in PRA from 12-day-old piglets. ACh stimulation caused dilation in PRA from 5- to 7-day- and 12-day-old but not 2-day-old animals, despite rapid and equivalent ACh-mediated eNOS phosphorylation (Ser1177) in all three age groups. Hsp90 inhibition abolished ACh-mediated dilation in PRA from the older piglets. ACh failed to stimulate Hsp90-eNOS binding in 2-day-old but induced a significant increase in Hsp90-eNOS coimmunoprecipitation in PRA from the older age groups, which was blocked by Hsp90 antagonism. We conclude that physical interactions between Hsp90 and eNOS mature over the first weeks of life, likely contributing to the postnatal fall in pulmonary vascular resistance and changes in agonist-induced pulmonary vascular responses characteristic of the early neonatal period.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume296
Issue number3
DOIs
StatePublished - Mar 2009
Externally publishedYes

Fingerprint

HSP90 Heat-Shock Proteins
Pulmonary Circulation
Nitric Oxide Synthase Type III
Pulmonary Artery
Dilatation
Constriction
Myography
Age Groups
Caveolin 1
Vascular Resistance
Blood Vessels
Proteins
Phosphorylation

Keywords

  • Akt
  • Development
  • Nitric oxide

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology

Cite this

Heat shock protein 90-eNOS interactions mature with postnatal age in the pulmonary circulation of the piglet. / Aschner, Judy L.; Zeng, Heng; Kaplowitz, Mark R.; Zhang, Yongmei; Slaughter, James C.; Fike, Candice D.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 296, No. 3, 03.2009.

Research output: Contribution to journalArticle

Aschner, Judy L. ; Zeng, Heng ; Kaplowitz, Mark R. ; Zhang, Yongmei ; Slaughter, James C. ; Fike, Candice D. / Heat shock protein 90-eNOS interactions mature with postnatal age in the pulmonary circulation of the piglet. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2009 ; Vol. 296, No. 3.
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