Guidelines for the use of flow cytometry and cell sorting in immunological studies

Andrea Cossarizza; Hyun Dong Chang; Andreas Radbruch; Mübeccel Akdis; Immanuel Andrä; Francesco Annunziato; Petra Bacher; Vincenzo Barnaba; Luca Battistini; Wolfgang M. Bauer; Sabine Baumgart; Burkhard Becher; Wolfgang Beisker; Claudia Berek; Alfonso Blanco; Giovanna Borsellino; Philip E. Boulais; Ryan R. Brinkman; Martin Büscher; Dirk H. Busch; Timothy P. Bushnell; Xuetao Cao; Andrea Cavani; Pratip K. Chattopadhyay; Qingyu Cheng; Sue Chow; Mario Clerici; Anne Cooke; Antonio Cosma; Lorenzo Cosmi; Ana Cumano; Van Duc Dang; Derek Davies; Sara De Biasi; Genny Del Zotto; Silvia Della Bella; Paolo Dellabona; Günnur Deniz; Mark Dessing; Andreas Diefenbach; James Di Santo; Francesco Dieli; Andreas Dolf; Vera S. Donnenberg; Thomas Dörner; Götz R.A. Ehrhardt; Elmar Endl; Pablo Engel; Britta Engelhardt; Charlotte Esser; Bart Everts; Anita Dreher; Christine S. Falk; Todd A. Fehniger; Andrew Filby; Simon Fillatreau; Marie Follo; Irmgard Förster; John Foster; Gemma A. Foulds; Paul S. Frenette; David Galbraith; Natalio Garbi; Maria Dolores García-Godoy; Jens Geginat; Kamran Ghoreschi; Lara Gibellini; Christoph Goettlinger; Carl S. Goodyear; Andrea Gori; Jane Grogan; Mor Gross; Andreas Grützkau; Daryl Grummitt; Jonas Hahn; Quirin Hammer; Anja E. Hauser; David L. Haviland; David Hedley; Guadalupe Herrera; Martin Herrmann; Falk Hiepe; Tristan Holland; Pleun Hombrink; Jessica P. Houston; Bimba F. Hoyer; Bo Huang; Christopher A. Hunter; Anna Iannone; Hans Martin Jäck; Beatriz Jávega; Stipan Jonjic; Kerstin Juelke; Steffen Jung; Toralf Kaiser; Tomas Kalina; Baerbel Keller; Srijit Khan; Deborah Kienhöfer; Thomas Kroneis; Désirée Kunkel; Christian Kurts; Pia Kvistborg; Joanne Lannigan; Olivier Lantz; Anis Larbi; Salome LeibundGut-Landmann; Michael D. Leipold; Megan K. Levings; Virginia Litwin; Yanling Liu; Michael Lohoff; Giovanna Lombardi; Lilly Lopez; Amy Lovett-Racke; Erik Lubberts; Burkhard Ludewig; Enrico Lugli; Holden T. Maecker; Glòria Martrus; Giuseppe Matarese; Christian Maueröder; Mairi McGrath; Iain McInnes; Henrik E. Mei; Fritz Melchers; Susanne Melzer; Dirk Mielenz; Kingston Mills; David Mirrer; Jenny Mjösberg; Jonni Moore; Barry Moran; Alessandro Moretta; Lorenzo Moretta; Tim R. Mosmann; Susann Müller; Werner Müller; Christian Münz; Gabriele Multhoff; Luis Enrique Munoz; Kenneth M. Murphy; Toshinori Nakayama; Milena Nasi; Christine Neudörfl; John Nolan; Sussan Nourshargh; José Enrique O'Connor; Wenjun Ouyang; Annette Oxenius; Raghav Palankar; Isabel Panse; Pärt Peterson; Christian Peth; Jordi Petriz; Daisy Philips; Winfried Pickl; Silvia Piconese; Marcello Pinti; A. Graham Pockley; Malgorzata Justyna Podolska; Carlo Pucillo; Sally A. Quataert; Timothy R.D.J. Radstake; Bartek Rajwa; Jonathan A. Rebhahn; Diether Recktenwald; Ester B.M. Remmerswaal; Katy Rezvani; Laura G. Rico; J. Paul Robinson; Chiara Romagnani; Anna Rubartelli; Beate Ruckert; Jürgen Ruland; Shimon Sakaguchi; Francisco Sala-de-Oyanguren; Yvonne Samstag; Sharon Sanderson; Birgit Sawitzki; Alexander Scheffold; Matthias Schiemann; Frank Schildberg; Esther Schimisky; Stephan A. Schmid; Steffen Schmitt; Kilian Schober; Thomas Schüler; Axel Ronald Schulz; Ton Schumacher; Cristiano Scotta; T. Vincent Shankey; Anat Shemer; Anna Katharina Simon; Josef Spidlen; Alan M. Stall; Regina Stark; Christina Stehle; Merle Stein; Tobit Steinmetz; Hannes Stockinger; Yousuke Takahama; Attila Tarnok; Zhi Gang Tian; Gergely Toldi; Julia Tornack; Elisabetta Traggiai; Joe Trotter; Henning Ulrich; Marlous van der Braber; René A.W. van Lier; Marcello Veldhoen; Salvador Vento-Asturias; Paulo Vieira; David Voehringer; Hans Dieter Volk; Konrad von Volkmann; Ari Waisman; Rachael Walker; Michael D. Ward; Klaus Warnatz; Sarah Warth; James V. Watson; Carsten Watzl; Leonie Wegener; Annika Wiedemann; Jürgen Wienands; Gerald Willimsky; James Wing; Peter Wurst; Liping Yu; Alice Yue; Qianjun Zhang; Yi Zhao; Susanne Ziegler; Jakob Zimmermann

doi:10.1002/eji.201646632

Guidelines for the use of flow cytometry and cell sorting in immunological studies

Andrea Cossarizza, Hyun Dong Chang, Andreas Radbruch, Mübeccel Akdis, Immanuel Andrä, Francesco Annunziato, Petra Bacher, Vincenzo Barnaba, Luca Battistini, Wolfgang M. Bauer, Sabine Baumgart, Burkhard Becher, Wolfgang Beisker, Claudia Berek, Alfonso Blanco, Giovanna Borsellino, Philip E. Boulais, Ryan R. Brinkman, Martin Büscher, Dirk H. BuschTimothy P. Bushnell, Xuetao Cao, Andrea Cavani, Pratip K. Chattopadhyay, Qingyu Cheng, Sue Chow, Mario Clerici, Anne Cooke, Antonio Cosma, Lorenzo Cosmi, Ana Cumano, Van Duc Dang, Derek Davies, Sara De Biasi, Genny Del Zotto, Silvia Della Bella, Paolo Dellabona, Günnur Deniz, Mark Dessing, Andreas Diefenbach, James Di Santo, Francesco Dieli, Andreas Dolf, Vera S. Donnenberg, Thomas Dörner, Götz R.A. Ehrhardt, Elmar Endl, Pablo Engel, Britta Engelhardt, Charlotte Esser, Bart Everts, Anita Dreher, Christine S. Falk, Todd A. Fehniger, Andrew Filby, Simon Fillatreau, Marie Follo, Irmgard Förster, John Foster, Gemma A. Foulds, Paul S. Frenette, David Galbraith, Natalio Garbi, Maria Dolores García-Godoy, Jens Geginat, Kamran Ghoreschi, Lara Gibellini, Christoph Goettlinger, Carl S. Goodyear, Andrea Gori, Jane Grogan, Mor Gross, Andreas Grützkau, Daryl Grummitt, Jonas Hahn, Quirin Hammer, Anja E. Hauser, David L. Haviland, David Hedley, Guadalupe Herrera, Martin Herrmann, Falk Hiepe, Tristan Holland, Pleun Hombrink, Jessica P. Houston, Bimba F. Hoyer, Bo Huang, Christopher A. Hunter, Anna Iannone, Hans Martin Jäck, Beatriz Jávega, Stipan Jonjic, Kerstin Juelke, Steffen Jung, Toralf Kaiser, Tomas Kalina, Baerbel Keller, Srijit Khan, Deborah Kienhöfer, Thomas Kroneis, Désirée Kunkel, Christian Kurts, Pia Kvistborg, Joanne Lannigan, Olivier Lantz, Anis Larbi, Salome LeibundGut-Landmann, Michael D. Leipold, Megan K. Levings, Virginia Litwin, Yanling Liu, Michael Lohoff, Giovanna Lombardi, Lilly Lopez, Amy Lovett-Racke, Erik Lubberts, Burkhard Ludewig, Enrico Lugli, Holden T. Maecker, Glòria Martrus, Giuseppe Matarese, Christian Maueröder, Mairi McGrath, Iain McInnes, Henrik E. Mei, Fritz Melchers, Susanne Melzer, Dirk Mielenz, Kingston Mills, David Mirrer, Jenny Mjösberg, Jonni Moore, Barry Moran, Alessandro Moretta, Lorenzo Moretta, Tim R. Mosmann, Susann Müller, Werner Müller, Christian Münz, Gabriele Multhoff, Luis Enrique Munoz, Kenneth M. Murphy, Toshinori Nakayama, Milena Nasi, Christine Neudörfl, John Nolan, Sussan Nourshargh, José Enrique O'Connor, Wenjun Ouyang, Annette Oxenius, Raghav Palankar, Isabel Panse, Pärt Peterson, Christian Peth, Jordi Petriz, Daisy Philips, Winfried Pickl, Silvia Piconese, Marcello Pinti, A. Graham Pockley, Malgorzata Justyna Podolska, Carlo Pucillo, Sally A. Quataert, Timothy R.D.J. Radstake, Bartek Rajwa, Jonathan A. Rebhahn, Diether Recktenwald, Ester B.M. Remmerswaal, Katy Rezvani, Laura G. Rico, J. Paul Robinson, Chiara Romagnani, Anna Rubartelli, Beate Ruckert, Jürgen Ruland, Shimon Sakaguchi, Francisco Sala-de-Oyanguren, Yvonne Samstag, Sharon Sanderson, Birgit Sawitzki, Alexander Scheffold, Matthias Schiemann, Frank Schildberg, Esther Schimisky, Stephan A. Schmid, Steffen Schmitt, Kilian Schober, Thomas Schüler, Axel Ronald Schulz, Ton Schumacher, Cristiano Scotta, T. Vincent Shankey, Anat Shemer, Anna Katharina Simon, Josef Spidlen, Alan M. Stall, Regina Stark, Christina Stehle, Merle Stein, Tobit Steinmetz, Hannes Stockinger, Yousuke Takahama, Attila Tarnok, Zhi Gang Tian, Gergely Toldi, Julia Tornack, Elisabetta Traggiai, Joe Trotter, Henning Ulrich, Marlous van der Braber, René A.W. van Lier, Marcello Veldhoen, Salvador Vento-Asturias, Paulo Vieira, David Voehringer, Hans Dieter Volk, Konrad von Volkmann, Ari Waisman, Rachael Walker, Michael D. Ward, Klaus Warnatz, Sarah Warth, James V. Watson, Carsten Watzl, Leonie Wegener, Annika Wiedemann, Jürgen Wienands, Gerald Willimsky, James Wing, Peter Wurst, Liping Yu, Alice Yue, Qianjun Zhang, Yi Zhao, Susanne Ziegler, Jakob Zimmermann

Medicine

Research output: Contribution to journal › Article › peer-review

413 Scopus citations

Original language	English (US)
Pages (from-to)	1584-1797
Number of pages	214
Journal	European Journal of Immunology
Volume	47
Issue number	10
DOIs	https://doi.org/10.1002/eji.201646632
State	Published - Oct 2017

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1002/eji.201646632

Cite this

Cossarizza, A., Chang, H. D., Radbruch, A., Akdis, M., Andrä, I., Annunziato, F., Bacher, P., Barnaba, V., Battistini, L., Bauer, W. M., Baumgart, S., Becher, B., Beisker, W., Berek, C., Blanco, A., Borsellino, G., Boulais, P. E., Brinkman, R. R., Büscher, M., ... Zimmermann, J. (2017). Guidelines for the use of flow cytometry and cell sorting in immunological studies. European Journal of Immunology, 47(10), 1584-1797. https://doi.org/10.1002/eji.201646632

Cossarizza, A, Chang, HD, Radbruch, A, Akdis, M, Andrä, I, Annunziato, F, Bacher, P, Barnaba, V, Battistini, L, Bauer, WM, Baumgart, S, Becher, B, Beisker, W, Berek, C, Blanco, A, Borsellino, G, Boulais, PE, Brinkman, RR, Büscher, M, Busch, DH, Bushnell, TP, Cao, X, Cavani, A, Chattopadhyay, PK, Cheng, Q, Chow, S, Clerici, M, Cooke, A, Cosma, A, Cosmi, L, Cumano, A, Dang, VD, Davies, D, De Biasi, S, Del Zotto, G, Della Bella, S, Dellabona, P, Deniz, G, Dessing, M, Diefenbach, A, Di Santo, J, Dieli, F, Dolf, A, Donnenberg, VS, Dörner, T, Ehrhardt, GRA, Endl, E, Engel, P, Engelhardt, B, Esser, C, Everts, B, Dreher, A, Falk, CS, Fehniger, TA, Filby, A, Fillatreau, S, Follo, M, Förster, I, Foster, J, Foulds, GA, Frenette, PS, Galbraith, D, Garbi, N, García-Godoy, MD, Geginat, J, Ghoreschi, K, Gibellini, L, Goettlinger, C, Goodyear, CS, Gori, A, Grogan, J, Gross, M, Grützkau, A, Grummitt, D, Hahn, J, Hammer, Q, Hauser, AE, Haviland, DL, Hedley, D, Herrera, G, Herrmann, M, Hiepe, F, Holland, T, Hombrink, P, Houston, JP, Hoyer, BF, Huang, B, Hunter, CA, Iannone, A, Jäck, HM, Jávega, B, Jonjic, S, Juelke, K, Jung, S, Kaiser, T, Kalina, T, Keller, B, Khan, S, Kienhöfer, D, Kroneis, T, Kunkel, D, Kurts, C, Kvistborg, P, Lannigan, J, Lantz, O, Larbi, A, LeibundGut-Landmann, S, Leipold, MD, Levings, MK, Litwin, V, Liu, Y, Lohoff, M, Lombardi, G, Lopez, L, Lovett-Racke, A, Lubberts, E, Ludewig, B, Lugli, E, Maecker, HT, Martrus, G, Matarese, G, Maueröder, C, McGrath, M, McInnes, I, Mei, HE, Melchers, F, Melzer, S, Mielenz, D, Mills, K, Mirrer, D, Mjösberg, J, Moore, J, Moran, B, Moretta, A, Moretta, L, Mosmann, TR, Müller, S, Müller, W, Münz, C, Multhoff, G, Munoz, LE, Murphy, KM, Nakayama, T, Nasi, M, Neudörfl, C, Nolan, J, Nourshargh, S, O'Connor, JE, Ouyang, W, Oxenius, A, Palankar, R, Panse, I, Peterson, P, Peth, C, Petriz, J, Philips, D, Pickl, W, Piconese, S, Pinti, M, Pockley, AG, Podolska, MJ, Pucillo, C, Quataert, SA, Radstake, TRDJ, Rajwa, B, Rebhahn, JA, Recktenwald, D, Remmerswaal, EBM, Rezvani, K, Rico, LG, Robinson, JP, Romagnani, C, Rubartelli, A, Ruckert, B, Ruland, J, Sakaguchi, S, Sala-de-Oyanguren, F, Samstag, Y, Sanderson, S, Sawitzki, B, Scheffold, A, Schiemann, M, Schildberg, F, Schimisky, E, Schmid, SA, Schmitt, S, Schober, K, Schüler, T, Schulz, AR, Schumacher, T, Scotta, C, Shankey, TV, Shemer, A, Simon, AK, Spidlen, J, Stall, AM, Stark, R, Stehle, C, Stein, M, Steinmetz, T, Stockinger, H, Takahama, Y, Tarnok, A, Tian, ZG, Toldi, G, Tornack, J, Traggiai, E, Trotter, J, Ulrich, H, van der Braber, M, van Lier, RAW, Veldhoen, M, Vento-Asturias, S, Vieira, P, Voehringer, D, Volk, HD, von Volkmann, K, Waisman, A, Walker, R, Ward, MD, Warnatz, K, Warth, S, Watson, JV, Watzl, C, Wegener, L, Wiedemann, A, Wienands, J, Willimsky, G, Wing, J, Wurst, P, Yu, L, Yue, A, Zhang, Q, Zhao, Y, Ziegler, S & Zimmermann, J 2017, 'Guidelines for the use of flow cytometry and cell sorting in immunological studies', European Journal of Immunology, vol. 47, no. 10, pp. 1584-1797. https://doi.org/10.1002/eji.201646632

@article{8586381c3cfc4b25b5a0a8083104be10,

title = "Guidelines for the use of flow cytometry and cell sorting in immunological studies",

author = "Andrea Cossarizza and Chang, {Hyun Dong} and Andreas Radbruch and M{\"u}beccel Akdis and Immanuel Andr{\"a} and Francesco Annunziato and Petra Bacher and Vincenzo Barnaba and Luca Battistini and Bauer, {Wolfgang M.} and Sabine Baumgart and Burkhard Becher and Wolfgang Beisker and Claudia Berek and Alfonso Blanco and Giovanna Borsellino and Boulais, {Philip E.} and Brinkman, {Ryan R.} and Martin B{\"u}scher and Busch, {Dirk H.} and Bushnell, {Timothy P.} and Xuetao Cao and Andrea Cavani and Chattopadhyay, {Pratip K.} and Qingyu Cheng and Sue Chow and Mario Clerici and Anne Cooke and Antonio Cosma and Lorenzo Cosmi and Ana Cumano and Dang, {Van Duc} and Derek Davies and {De Biasi}, Sara and {Del Zotto}, Genny and {Della Bella}, Silvia and Paolo Dellabona and G{\"u}nnur Deniz and Mark Dessing and Andreas Diefenbach and {Di Santo}, James and Francesco Dieli and Andreas Dolf and Donnenberg, {Vera S.} and Thomas D{\"o}rner and Ehrhardt, {G{\"o}tz R.A.} and Elmar Endl and Pablo Engel and Britta Engelhardt and Charlotte Esser and Bart Everts and Anita Dreher and Falk, {Christine S.} and Fehniger, {Todd A.} and Andrew Filby and Simon Fillatreau and Marie Follo and Irmgard F{\"o}rster and John Foster and Foulds, {Gemma A.} and Frenette, {Paul S.} and David Galbraith and Natalio Garbi and Garc{\'i}a-Godoy, {Maria Dolores} and Jens Geginat and Kamran Ghoreschi and Lara Gibellini and Christoph Goettlinger and Goodyear, {Carl S.} and Andrea Gori and Jane Grogan and Mor Gross and Andreas Gr{\"u}tzkau and Daryl Grummitt and Jonas Hahn and Quirin Hammer and Hauser, {Anja E.} and Haviland, {David L.} and David Hedley and Guadalupe Herrera and Martin Herrmann and Falk Hiepe and Tristan Holland and Pleun Hombrink and Houston, {Jessica P.} and Hoyer, {Bimba F.} and Bo Huang and Hunter, {Christopher A.} and Anna Iannone and J{\"a}ck, {Hans Martin} and Beatriz J{\'a}vega and Stipan Jonjic and Kerstin Juelke and Steffen Jung and Toralf Kaiser and Tomas Kalina and Baerbel Keller and Srijit Khan and Deborah Kienh{\"o}fer and Thomas Kroneis and D{\'e}sir{\'e}e Kunkel and Christian Kurts and Pia Kvistborg and Joanne Lannigan and Olivier Lantz and Anis Larbi and Salome LeibundGut-Landmann and Leipold, {Michael D.} and Levings, {Megan K.} and Virginia Litwin and Yanling Liu and Michael Lohoff and Giovanna Lombardi and Lilly Lopez and Amy Lovett-Racke and Erik Lubberts and Burkhard Ludewig and Enrico Lugli and Maecker, {Holden T.} and Gl{\`o}ria Martrus and Giuseppe Matarese and Christian Mauer{\"o}der and Mairi McGrath and Iain McInnes and Mei, {Henrik E.} and Fritz Melchers and Susanne Melzer and Dirk Mielenz and Kingston Mills and David Mirrer and Jenny Mj{\"o}sberg and Jonni Moore and Barry Moran and Alessandro Moretta and Lorenzo Moretta and Mosmann, {Tim R.} and Susann M{\"u}ller and Werner M{\"u}ller and Christian M{\"u}nz and Gabriele Multhoff and Munoz, {Luis Enrique} and Murphy, {Kenneth M.} and Toshinori Nakayama and Milena Nasi and Christine Neud{\"o}rfl and John Nolan and Sussan Nourshargh and O'Connor, {Jos{\'e} Enrique} and Wenjun Ouyang and Annette Oxenius and Raghav Palankar and Isabel Panse and P{\"a}rt Peterson and Christian Peth and Jordi Petriz and Daisy Philips and Winfried Pickl and Silvia Piconese and Marcello Pinti and Pockley, {A. Graham} and Podolska, {Malgorzata Justyna} and Carlo Pucillo and Quataert, {Sally A.} and Radstake, {Timothy R.D.J.} and Bartek Rajwa and Rebhahn, {Jonathan A.} and Diether Recktenwald and Remmerswaal, {Ester B.M.} and Katy Rezvani and Rico, {Laura G.} and Robinson, {J. Paul} and Chiara Romagnani and Anna Rubartelli and Beate Ruckert and J{\"u}rgen Ruland and Shimon Sakaguchi and Francisco Sala-de-Oyanguren and Yvonne Samstag and Sharon Sanderson and Birgit Sawitzki and Alexander Scheffold and Matthias Schiemann and Frank Schildberg and Esther Schimisky and Schmid, {Stephan A.} and Steffen Schmitt and Kilian Schober and Thomas Sch{\"u}ler and Schulz, {Axel Ronald} and Ton Schumacher and Cristiano Scotta and Shankey, {T. Vincent} and Anat Shemer and Simon, {Anna Katharina} and Josef Spidlen and Stall, {Alan M.} and Regina Stark and Christina Stehle and Merle Stein and Tobit Steinmetz and Hannes Stockinger and Yousuke Takahama and Attila Tarnok and Tian, {Zhi Gang} and Gergely Toldi and Julia Tornack and Elisabetta Traggiai and Joe Trotter and Henning Ulrich and {van der Braber}, Marlous and {van Lier}, {Ren{\'e} A.W.} and Marcello Veldhoen and Salvador Vento-Asturias and Paulo Vieira and David Voehringer and Volk, {Hans Dieter} and {von Volkmann}, Konrad and Ari Waisman and Rachael Walker and Ward, {Michael D.} and Klaus Warnatz and Sarah Warth and Watson, {James V.} and Carsten Watzl and Leonie Wegener and Annika Wiedemann and J{\"u}rgen Wienands and Gerald Willimsky and James Wing and Peter Wurst and Liping Yu and Alice Yue and Qianjun Zhang and Yi Zhao and Susanne Ziegler and Jakob Zimmermann",

note = "Funding Information: Mairi Mc Grath and Regina Stark thank Francesco Siracusa and Patrick Maschmeyer for providing data and Klaas van Gisbergen for helpful discussions. Philip E. Boulais and Paul S. Frenette are grateful to Dr. Sandra Pinho for helpful comments and suggestions. They thank the National Institutes of Health for their support (R01 grants DK056638, HL116340, HL097819 to P.S.F). They also thank the New York State Department of Health (NYSTEM Program) for shared facility (C029154) and research support (N13G-262) and the Leukemia and Lymphoma Society{\textquoteright}s Translational Research Program. Funding Information: Acknowledgements: Enrico Lugli and Pratip K. Chattopadhyay were supported by grants from the Fondazione Cariplo (Grant Ricerca Biomedica 2012/0683), the Italian Ministry of Health (Bando Giovani Ricercatori GR-2011-02347324) and the European Union Marie Curie Career Integration Grant 322093 (all to E.L.). E.L. and P.K.C. are International Society for the Advancement of Cytometry (ISAC) Marylou Ingram scholars. Alice Yue and Ryan R. Brinkman were funded by Genome BC and NSERC. Klaus Warnatz received funding from the German Federal Ministry of Education and Research (BMBF 01EO1303) and the Deutsche Forschungsgemeinschaft (DECIDE, DFG WA 1597/4-1 and the TRR130). The Jung laboratory is supported by funds of the ERC and ISF. Henrik Mei is a 2017-2021 ISAC scholar. Antonio Cosma is supported by the French government program: “Investissement d{\textquoteright}avenir: Equipements d{\textquoteright}Excellence” (EQUIPEX)-2010 FlowCyTech, Grant number: ANR-10-EQPX-02-01. Henrik Mei is supported by the Deutsche Forschungsgemeinschaft (DFG; grants Me3644/5-1 and TRR130/TP24). Funding Information: The Immunology Database and Analysis Portal (ImmPort) system provides an archive of immunology research data generated by investigators mainly funded through the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), Division of Allergy, Immunology, and Transplantation (DAIT). It is an extensive data warehouse containing an integration of experimental and clinical trial data generated by dozens of assay types, including 63 flow cytometry and 5 CyTOF data sets. In addition, the ImmPort system also provides data analysis tools and it contains implicit knowledge and {\textquoteleft}{\textquoteleft}best practices{\textquoteright}{\textquoteright} for clinical and genomic studies in the form of nearly 50 templates for data deposition, management, and dissemination. ImmPort has been developed under the Bioinformatics Integration Support Contract (BISC) by the Northrop Grumman Information Technology Health",

year = "2017",

month = oct,

doi = "10.1002/eji.201646632",

language = "English (US)",

volume = "47",

pages = "1584--1797",

journal = "European Journal of Immunology",

issn = "0014-2980",

publisher = "Wiley-VCH Verlag",

number = "10",

}

TY - JOUR

T1 - Guidelines for the use of flow cytometry and cell sorting in immunological studies

AU - Cossarizza, Andrea

AU - Chang, Hyun Dong

AU - Radbruch, Andreas

AU - Akdis, Mübeccel

AU - Andrä, Immanuel

AU - Annunziato, Francesco

AU - Bacher, Petra

AU - Barnaba, Vincenzo

AU - Battistini, Luca

AU - Bauer, Wolfgang M.

AU - Baumgart, Sabine

AU - Becher, Burkhard

AU - Beisker, Wolfgang

AU - Berek, Claudia

AU - Blanco, Alfonso

AU - Borsellino, Giovanna

AU - Boulais, Philip E.

AU - Brinkman, Ryan R.

AU - Büscher, Martin

AU - Busch, Dirk H.

AU - Bushnell, Timothy P.

AU - Cao, Xuetao

AU - Cavani, Andrea

AU - Chattopadhyay, Pratip K.

AU - Cheng, Qingyu

AU - Chow, Sue

AU - Clerici, Mario

AU - Cooke, Anne

AU - Cosma, Antonio

AU - Cosmi, Lorenzo

AU - Cumano, Ana

AU - Dang, Van Duc

AU - Davies, Derek

AU - De Biasi, Sara

AU - Del Zotto, Genny

AU - Della Bella, Silvia

AU - Dellabona, Paolo

AU - Deniz, Günnur

AU - Dessing, Mark

AU - Diefenbach, Andreas

AU - Di Santo, James

AU - Dieli, Francesco

AU - Dolf, Andreas

AU - Donnenberg, Vera S.

AU - Dörner, Thomas

AU - Ehrhardt, Götz R.A.

AU - Endl, Elmar

AU - Engel, Pablo

AU - Engelhardt, Britta

AU - Esser, Charlotte

AU - Everts, Bart

AU - Dreher, Anita

AU - Falk, Christine S.

AU - Fehniger, Todd A.

AU - Filby, Andrew

AU - Fillatreau, Simon

AU - Follo, Marie

AU - Förster, Irmgard

AU - Foster, John

AU - Foulds, Gemma A.

AU - Frenette, Paul S.

AU - Galbraith, David

AU - Garbi, Natalio

AU - García-Godoy, Maria Dolores

AU - Geginat, Jens

AU - Ghoreschi, Kamran

AU - Gibellini, Lara

AU - Goettlinger, Christoph

AU - Goodyear, Carl S.

AU - Gori, Andrea

AU - Grogan, Jane

AU - Gross, Mor

AU - Grützkau, Andreas

AU - Grummitt, Daryl

AU - Hahn, Jonas

AU - Hammer, Quirin

AU - Hauser, Anja E.

AU - Haviland, David L.

AU - Hedley, David

AU - Herrera, Guadalupe

AU - Herrmann, Martin

AU - Hiepe, Falk

AU - Holland, Tristan

AU - Hombrink, Pleun

AU - Houston, Jessica P.

AU - Hoyer, Bimba F.

AU - Huang, Bo

AU - Hunter, Christopher A.

AU - Iannone, Anna

AU - Jäck, Hans Martin

AU - Jávega, Beatriz

AU - Jonjic, Stipan

AU - Juelke, Kerstin

AU - Jung, Steffen

AU - Kaiser, Toralf

AU - Kalina, Tomas

AU - Keller, Baerbel

AU - Khan, Srijit

AU - Kienhöfer, Deborah

AU - Kroneis, Thomas

AU - Kunkel, Désirée

AU - Kurts, Christian

AU - Kvistborg, Pia

AU - Lannigan, Joanne

AU - Lantz, Olivier

AU - Larbi, Anis

AU - LeibundGut-Landmann, Salome

AU - Leipold, Michael D.

AU - Levings, Megan K.

AU - Litwin, Virginia

AU - Liu, Yanling

AU - Lohoff, Michael

AU - Lombardi, Giovanna

AU - Lopez, Lilly

AU - Lovett-Racke, Amy

AU - Lubberts, Erik

AU - Ludewig, Burkhard

AU - Lugli, Enrico

AU - Maecker, Holden T.

AU - Martrus, Glòria

AU - Matarese, Giuseppe

AU - Maueröder, Christian

AU - McGrath, Mairi

AU - McInnes, Iain

AU - Mei, Henrik E.

AU - Melchers, Fritz

AU - Melzer, Susanne

AU - Mielenz, Dirk

AU - Mills, Kingston

AU - Mirrer, David

AU - Mjösberg, Jenny

AU - Moore, Jonni

AU - Moran, Barry

AU - Moretta, Alessandro

AU - Moretta, Lorenzo

AU - Mosmann, Tim R.

AU - Müller, Susann

AU - Müller, Werner

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N1 - Funding Information: Mairi Mc Grath and Regina Stark thank Francesco Siracusa and Patrick Maschmeyer for providing data and Klaas van Gisbergen for helpful discussions. Philip E. Boulais and Paul S. Frenette are grateful to Dr. Sandra Pinho for helpful comments and suggestions. They thank the National Institutes of Health for their support (R01 grants DK056638, HL116340, HL097819 to P.S.F). They also thank the New York State Department of Health (NYSTEM Program) for shared facility (C029154) and research support (N13G-262) and the Leukemia and Lymphoma Society’s Translational Research Program. Funding Information: Acknowledgements: Enrico Lugli and Pratip K. Chattopadhyay were supported by grants from the Fondazione Cariplo (Grant Ricerca Biomedica 2012/0683), the Italian Ministry of Health (Bando Giovani Ricercatori GR-2011-02347324) and the European Union Marie Curie Career Integration Grant 322093 (all to E.L.). E.L. and P.K.C. are International Society for the Advancement of Cytometry (ISAC) Marylou Ingram scholars. Alice Yue and Ryan R. Brinkman were funded by Genome BC and NSERC. Klaus Warnatz received funding from the German Federal Ministry of Education and Research (BMBF 01EO1303) and the Deutsche Forschungsgemeinschaft (DECIDE, DFG WA 1597/4-1 and the TRR130). The Jung laboratory is supported by funds of the ERC and ISF. Henrik Mei is a 2017-2021 ISAC scholar. Antonio Cosma is supported by the French government program: “Investissement d’avenir: Equipements d’Excellence” (EQUIPEX)-2010 FlowCyTech, Grant number: ANR-10-EQPX-02-01. Henrik Mei is supported by the Deutsche Forschungsgemeinschaft (DFG; grants Me3644/5-1 and TRR130/TP24). Funding Information: The Immunology Database and Analysis Portal (ImmPort) system provides an archive of immunology research data generated by investigators mainly funded through the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), Division of Allergy, Immunology, and Transplantation (DAIT). It is an extensive data warehouse containing an integration of experimental and clinical trial data generated by dozens of assay types, including 63 flow cytometry and 5 CyTOF data sets. In addition, the ImmPort system also provides data analysis tools and it contains implicit knowledge and ‘‘best practices’’ for clinical and genomic studies in the form of nearly 50 templates for data deposition, management, and dissemination. ImmPort has been developed under the Bioinformatics Integration Support Contract (BISC) by the Northrop Grumman Information Technology Health

PY - 2017/10

Y1 - 2017/10

UR - http://www.scopus.com/inward/record.url?scp=85032278767&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032278767&partnerID=8YFLogxK

U2 - 10.1002/eji.201646632

DO - 10.1002/eji.201646632

M3 - Article

C2 - 29023707

AN - SCOPUS:85032278767

SN - 0014-2980

VL - 47

SP - 1584

EP - 1797

JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 10

ER -

Guidelines for the use of flow cytometry and cell sorting in immunological studies

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