GTP-Binding proteins in brain and neutrophil are tethered to the plasma membrane via their amino termini

Brock Eide, Peter Gierschik, Graeme Milligan, Ian Mullaney, Cecilia Unson, Paul Goldsmith, Allen Spiegel

Research output: Contribution to journalArticle

30 Scopus citations


Using specific antisera raised against synthetic peptides, we find that three distinct GTP-binding protein alpha subunits remain bound to the plasma membrane even after activation with nonhydrolyzable GTP analog. Trypsin cleaves each alpha subunit at a site near the amino-terminus, and quantitatively releases the large fragment (comprising all but an amino-terminal 2kDa piece) from the membrane. Our results indicate that alpha subunits are essentially cytoplasmic proteins tethered to the inner surface of the membrane via an amino terminal stalk.

Original languageEnglish (US)
Pages (from-to)1398-1405
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - Nov 13 1987
Externally publishedYes


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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