Granulomatous skin lesions in moray eels caused by a novel Mycobacterium species related to Mycobacterium triplex

Lawrence H. Herbst, S. F. Costa, Louis M. Weiss, L. K. Johnson, J. Bartell, R. Davis, M. Walsh, Michael H. Levi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

An outbreak of granulomatous dermatitis was investigated in a captive population of moray eels. The affected eels had florid skin nodules concentrated around the head and trunk. Histopathological examination revealed extensive granulomatous inflammation within the dermis and subcutaneous fascial plane between the fat and axial musculature. Acid-fast rods were detected within the smallest lesions, which were presumably the ones that had developed earliest. Eventually, after several months of incubation at room temperature, a very slowly growing acid-fast organism was isolated. Sequencing of the 16S rRNA gene identified it as a Mycobacterium species closely related (0.59% divergence) to M. triplex, an SAV mycobacterium. Intradermal inoculation of healthy green moray eels with this organism reliably reproduced the lesion. Experimentally induced granulomatous dermatitis appeared within 2 weeks of inoculation and slowly but progressively expanded during the 2 months of the experiment. Live organisms were recovered from these lesions at all time points, fulfilling Koch's postulates for this bacterium. In a retrospective study of tissues collected between 1993 and 1999 from five spontaneous disease cases, acid-fast rods were consistently found within lesions, and a nested PCR for the rRNA gene also demonstrated the presence of mycobacteria within affected tissues.

Original languageEnglish (US)
Pages (from-to)4639-4646
Number of pages8
JournalInfection and Immunity
Volume69
Issue number7
DOIs
StatePublished - 2001

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Eels
Mycobacterium
Dermatitis
rRNA Genes
Skin
Acids
Dermis
Disease Outbreaks
Retrospective Studies
Fats
Head
Inflammation
Bacteria
Polymerase Chain Reaction
Temperature
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Granulomatous skin lesions in moray eels caused by a novel Mycobacterium species related to Mycobacterium triplex. / Herbst, Lawrence H.; Costa, S. F.; Weiss, Louis M.; Johnson, L. K.; Bartell, J.; Davis, R.; Walsh, M.; Levi, Michael H.

In: Infection and Immunity, Vol. 69, No. 7, 2001, p. 4639-4646.

Research output: Contribution to journalArticle

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AU - Levi, Michael H.

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