Grafts of extra-adrenal chromaffin cells as aggregates show better survival rate and regenerative effects on parkinsonian rats than dispersed cell grafts

B. Galan-Rodriguez, A. del-Marco, J. A. Flores, S. Ramiro-Fuentes, R. Gonzalez-Aparicio, I. Tunez, Inmaculada Tasset, E. Fernandez-Espejo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The objective was to discern the neuroregenerative effect of grafts of extra-adrenal cells of the Zuckerkandl's paraganglion (ZP) in the nigrostriatal circuit, by using the retrograde model of parkinsonism in rats. The antiparkinsonian efficacy of two types of grafting procedures was studied (cell aggregates vs. dispersed cells), and GDNF and TGFβ1 (dopaminotrophic factors) as well as dopamine presence in extra-adrenal tissue was analyzed. Extra-adrenal chromaffin cells are noradrenergics, tissue dopamine is low, and they express both GDNF and TGFβ1. Grafts of cell aggregates, not of dispersed cells, exerted a trophic regeneration of the host striatum, leading to amelioration of motor deficits. Sprouting of spared dopaminergic fibers within the striatum, reduction of dopamine axon degeneration, and/or enhanced phenotypic expression of TH would explain striatal regeneration. Grafted cells as aggregates showed a better survival rate than dispersed cells, and they express higher levels of GDNF. Higher survivability and GDNF content together with the neurorestorative and dopaminotrophic action of both GDNF and TGFβ1 could account for striatal recovery and functional amelioration after grafting ZP cell aggregates. Finally, nigral degeneration and partial degeneration of ventral tegmental area were not precluded after transplantation, indicating that the trophic effect of grafts was local within the host striatum.

Original languageEnglish (US)
Pages (from-to)529-542
Number of pages14
JournalNeurobiology of Disease
Volume29
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Chromaffin Cells
Glial Cell Line-Derived Neurotrophic Factor
Transplants
Corpus Striatum
Dopamine
Regeneration
Antiparkinson Agents
Ventral Tegmental Area
Parkinsonian Disorders
Substantia Nigra
Axons
Transplantation

Keywords

  • Extra-adrenal
  • GDNF
  • Graft
  • Parkinson
  • Regeneration
  • TGFβ
  • Trophic
  • Zuckerkandl's paraganglion

ASJC Scopus subject areas

  • Neurology

Cite this

Galan-Rodriguez, B., del-Marco, A., Flores, J. A., Ramiro-Fuentes, S., Gonzalez-Aparicio, R., Tunez, I., ... Fernandez-Espejo, E. (2008). Grafts of extra-adrenal chromaffin cells as aggregates show better survival rate and regenerative effects on parkinsonian rats than dispersed cell grafts. Neurobiology of Disease, 29(3), 529-542. https://doi.org/10.1016/j.nbd.2007.11.009

Grafts of extra-adrenal chromaffin cells as aggregates show better survival rate and regenerative effects on parkinsonian rats than dispersed cell grafts. / Galan-Rodriguez, B.; del-Marco, A.; Flores, J. A.; Ramiro-Fuentes, S.; Gonzalez-Aparicio, R.; Tunez, I.; Tasset, Inmaculada; Fernandez-Espejo, E.

In: Neurobiology of Disease, Vol. 29, No. 3, 03.2008, p. 529-542.

Research output: Contribution to journalArticle

Galan-Rodriguez, B, del-Marco, A, Flores, JA, Ramiro-Fuentes, S, Gonzalez-Aparicio, R, Tunez, I, Tasset, I & Fernandez-Espejo, E 2008, 'Grafts of extra-adrenal chromaffin cells as aggregates show better survival rate and regenerative effects on parkinsonian rats than dispersed cell grafts', Neurobiology of Disease, vol. 29, no. 3, pp. 529-542. https://doi.org/10.1016/j.nbd.2007.11.009
Galan-Rodriguez, B. ; del-Marco, A. ; Flores, J. A. ; Ramiro-Fuentes, S. ; Gonzalez-Aparicio, R. ; Tunez, I. ; Tasset, Inmaculada ; Fernandez-Espejo, E. / Grafts of extra-adrenal chromaffin cells as aggregates show better survival rate and regenerative effects on parkinsonian rats than dispersed cell grafts. In: Neurobiology of Disease. 2008 ; Vol. 29, No. 3. pp. 529-542.
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