GP300SCA is not a high affinity Notch ligand

E. Chiang Lee, Nicholas E. Baker

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The Notch protooncogene encodes a receptor important for determination and differentiation of many cell types, and is conserved between vertebrates and invertebrates. It has been suggested that the secreted protein scabrous (sca) might be a Notch ligand acting in the peripheral nervous system. The sca protein was purified and a cell line expressing 18,000 Notch molecules per cell surface used to test sca binding by coimmunoprecipitation, cell adhesion assays, and binding with labeled sca. No interaction was detected between gp300sca and Notch or the related protein Delta, suggesting that sca acts through a distinct mechanism.

Original languageEnglish (US)
Pages (from-to)720-725
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume225
Issue number3
DOIs
StatePublished - Aug 23 1996

Fingerprint

Cells
Ligands
Cell adhesion
Peripheral Nervous System
Neurology
Invertebrates
Cell Adhesion
Vertebrates
Cell Differentiation
Assays
Proteins
Cell Line
Molecules
delta protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

GP300SCA is not a high affinity Notch ligand. / Lee, E. Chiang; Baker, Nicholas E.

In: Biochemical and Biophysical Research Communications, Vol. 225, No. 3, 23.08.1996, p. 720-725.

Research output: Contribution to journalArticle

@article{13ff8692021c49a5aaf4e805c67ffe63,
title = "GP300SCA is not a high affinity Notch ligand",
abstract = "The Notch protooncogene encodes a receptor important for determination and differentiation of many cell types, and is conserved between vertebrates and invertebrates. It has been suggested that the secreted protein scabrous (sca) might be a Notch ligand acting in the peripheral nervous system. The sca protein was purified and a cell line expressing 18,000 Notch molecules per cell surface used to test sca binding by coimmunoprecipitation, cell adhesion assays, and binding with labeled sca. No interaction was detected between gp300sca and Notch or the related protein Delta, suggesting that sca acts through a distinct mechanism.",
author = "Lee, {E. Chiang} and Baker, {Nicholas E.}",
year = "1996",
month = "8",
day = "23",
doi = "10.1006/bbrc.1996.1241",
language = "English (US)",
volume = "225",
pages = "720--725",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - GP300SCA is not a high affinity Notch ligand

AU - Lee, E. Chiang

AU - Baker, Nicholas E.

PY - 1996/8/23

Y1 - 1996/8/23

N2 - The Notch protooncogene encodes a receptor important for determination and differentiation of many cell types, and is conserved between vertebrates and invertebrates. It has been suggested that the secreted protein scabrous (sca) might be a Notch ligand acting in the peripheral nervous system. The sca protein was purified and a cell line expressing 18,000 Notch molecules per cell surface used to test sca binding by coimmunoprecipitation, cell adhesion assays, and binding with labeled sca. No interaction was detected between gp300sca and Notch or the related protein Delta, suggesting that sca acts through a distinct mechanism.

AB - The Notch protooncogene encodes a receptor important for determination and differentiation of many cell types, and is conserved between vertebrates and invertebrates. It has been suggested that the secreted protein scabrous (sca) might be a Notch ligand acting in the peripheral nervous system. The sca protein was purified and a cell line expressing 18,000 Notch molecules per cell surface used to test sca binding by coimmunoprecipitation, cell adhesion assays, and binding with labeled sca. No interaction was detected between gp300sca and Notch or the related protein Delta, suggesting that sca acts through a distinct mechanism.

UR - http://www.scopus.com/inward/record.url?scp=0030598853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030598853&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1996.1241

DO - 10.1006/bbrc.1996.1241

M3 - Article

C2 - 8780680

AN - SCOPUS:0030598853

VL - 225

SP - 720

EP - 725

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -