Glycosylation of the major polar tube protein of Encephalitozoon cuniculi

Boumediene Bouzahzah, Louis M. Weiss

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To infect their host cells the Microsporidia use a unique invasion organelle, the polar tube complex. During infection, the organism is injected into the host cell through the hollow polar tube formed during spore germination. Currently, three proteins, PTP1, PTP2, and PTP3 have been identified by immunological and molecular techniques as being components of this structure. Genomic data suggests that Microsporidia are capable of O-linked, but not N-linked glycosylation as a post-translational protein modification. Cells were infected with Encephalitozoon cunicuili, labeled with radioactive mannose or glucosamine, and the polar tube proteins were examined for glycosylation. PTP1 was clearly demonstrated to be mannosylated consistent with 0-glycosylation. In addition, it was evident that several other proteins were mannosylated, but no labeling was seen with glucosamine. The observed post-translational mannosylation of PTP1 may be involved in the functional properties of the polar tube, including its adherence to host cells during penetration.

Original languageEnglish (US)
Pages (from-to)761-764
Number of pages4
JournalParasitology Research
Volume107
Issue number3
DOIs
StatePublished - Aug 2010

Fingerprint

Encephalitozoon cuniculi
glycosylation
Glycosylation
Microsporidia
glucosamine
Glucosamine
Proteins
Encephalitozoon
proteins
cells
Immunologic Techniques
spore germination
Post Translational Protein Processing
Mannose
Germination
Spores
mannose
Organelles
functional properties
organelles

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Glycosylation of the major polar tube protein of Encephalitozoon cuniculi. / Bouzahzah, Boumediene; Weiss, Louis M.

In: Parasitology Research, Vol. 107, No. 3, 08.2010, p. 761-764.

Research output: Contribution to journalArticle

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