Glycerol metabolism in humans: validation of2H- and13C-labelled tracers

D. E. Matthews, G. R. Pesola, V. Kvetan

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

For a tracer to be valid it must follow the metabolism of the tracee without distortion. Especially when the tracer contains several deuterium substitutions, the tracer can be altered or degraded differently from the metabolite it is to trace or be subject to distorting isotope effects. To determine whether2H5-glycerol is a valid tracer for following glycerol kinetics,2H5-glycerol and [2-13C] glycerol tracers were infused simultaneously in six healthy postabsorptive adult subjects. After 90 min of tracer infusion, epinephrine was also infused for 60 min to stimulate lipolysis and increase glycerol flux. Glycerol flux increased from 2.2±0.3 to 6.7±0.4 μmol/kg per minute (with the13C tracer) and from 2.2±0.3 to 6.7±0.3 μmol/kg per minute (with the2H tracer) when epinephrine was infused. There was no significant difference in glycerol flux measured with the2H tracer compared to the13C tracer either under basal or a stimulated flux condition. These results indicate that2H5-glycerol is a valid tracer for measuring glycerol metabolism in humans.

Original languageEnglish (US)
Pages (from-to)179-184
Number of pages6
JournalActa Diabetologica
Volume28
Issue number2
DOIs
StatePublished - Jun 1991

Keywords

  • C-glycerol
  • Glycerol kinetics
  • H-glycerol
  • Stable isotope tracers

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Fingerprint Dive into the research topics of 'Glycerol metabolism in humans: validation of<sup>2</sup>H- and<sup>13</sup>C-labelled tracers'. Together they form a unique fingerprint.

Cite this