Genotyping the Prop-1 mutation in Ames dwarf mice

Martijn E.T. Dollé, Wendy K. Snyder, Jan Vijg

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The Ames dwarf mouse phenotype is based on a homozygous single gene mutation in the Prop-1 gene that markedly extends life span. Since its discovery, interest in breeding these mice as a model to study retardation of aging has increased dramatically. However, the homozygous Prop-1 mutants are infertile, which necessitates breeding heterozygotes. Heterozygotes cannot be distinguished from the wildtype, while the homozygote dwarf phenotype only becomes apparent after about 3 weeks. Hence, there is a need for a simple test to genotype individual animals at an early stage for the absence or presence of one or two copies of the Prop-1 mutant gene. Here we present a Prop-1 genotyping protocol, based on a PCR reaction followed by a PflMI digestion.

Original languageEnglish (US)
Pages (from-to)1915-1918
Number of pages4
JournalMechanisms of Ageing and Development
Volume122
Issue number15
DOIs
StatePublished - Sep 22 2001
Externally publishedYes

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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