TY - JOUR
T1 - Genotyping the Prop-1 mutation in Ames dwarf mice
AU - Dollé, Martijn E.T.
AU - Snyder, Wendy K.
AU - Vijg, Jan
N1 - Funding Information:
We thank Dr A. Bartke for kindly providin0g us with Ames dwarf breeders and tissues, and critical reading of the manuscript. This work was supported by NIH grants AG 17242 and AG 18923.
PY - 2001
Y1 - 2001
N2 - The Ames dwarf mouse phenotype is based on a homozygous single gene mutation in the Prop-1 gene that markedly extends life span. Since its discovery, interest in breeding these mice as a model to study retardation of aging has increased dramatically. However, the homozygous Prop-1 mutants are infertile, which necessitates breeding heterozygotes. Heterozygotes cannot be distinguished from the wildtype, while the homozygote dwarf phenotype only becomes apparent after about 3 weeks. Hence, there is a need for a simple test to genotype individual animals at an early stage for the absence or presence of one or two copies of the Prop-1 mutant gene. Here we present a Prop-1 genotyping protocol, based on a PCR reaction followed by a PflMI digestion.
AB - The Ames dwarf mouse phenotype is based on a homozygous single gene mutation in the Prop-1 gene that markedly extends life span. Since its discovery, interest in breeding these mice as a model to study retardation of aging has increased dramatically. However, the homozygous Prop-1 mutants are infertile, which necessitates breeding heterozygotes. Heterozygotes cannot be distinguished from the wildtype, while the homozygote dwarf phenotype only becomes apparent after about 3 weeks. Hence, there is a need for a simple test to genotype individual animals at an early stage for the absence or presence of one or two copies of the Prop-1 mutant gene. Here we present a Prop-1 genotyping protocol, based on a PCR reaction followed by a PflMI digestion.
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U2 - 10.1016/S0047-6374(01)00282-2
DO - 10.1016/S0047-6374(01)00282-2
M3 - Article
C2 - 11557289
AN - SCOPUS:0034839877
SN - 0047-6374
VL - 122
SP - 1915
EP - 1918
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
IS - 15
ER -