Genomic landscape of colorectal mucosa and adenomas

Ester Borras, F. Anthony San Lucas, Kyle Chang, Ruoji Zhou, Gita Masand, Jerry Fowler, Maureen E. Mork, Y. Nancy You, Melissa W. Taggart, Florencia McAllister, David A. Jones, Gareth E. Davies, Winfried Edelmann, Erik A. Ehli, Patrick M. Lynch, Ernest T. Hawk, Gabriel Capella, Paul Scheet, Eduardo Vilar

Research output: Contribution to journalArticle

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Abstract

The molecular basis of the adenoma-to-carcinoma transition has been deduced using comparative analysis of genetic alterations observed through the sequential steps of intestinal carcinogenesis. However, comprehensive genomic analyses of adenomas and at-risk mucosa are still lacking. Therefore, our aim was to characterize the genomic landscape of colonic atrisk mucosa and adenomas. We analyzed the mutation profile and copy number changes of 25 adenomas and adjacent mucosa from 12 familial adenomatous polyposis patients using whole-exome sequencing and validated allelic imbalances (AI) in 37 adenomas using SNP arrays. We assessed for evidence of clonality and performed estimations on the proportions of driver and passenger mutations using a systems biology approach. Adenomas had lower mutational rates than did colorectal cancers and showed recurrent alterations in known cancer driver genes (APC, KRAS, FBXW7, TCF7L2) and AIs in chromosomes 5, 7, and 13. Moreover, 80% of adenomas had somatic alterations in WNT pathway genes. Adenomas displayed evidence of multiclonality similar to stage I carcinomas. Strong correlations between mutational rate and patient age were observed in at-risk mucosa and adenomas. Our data indicate that at least 23% of somatic mutations are present in at-risk mucosa prior to adenoma initiation. The genomic profiles of at-risk mucosa and adenomas illustrate the evolution from normal tissue to carcinoma via greater resolution of molecular changes at the inflection point of premalignant lesions. Furthermore, substantial genomic variation exists in at-risk mucosa before adenoma formation, and deregulation of the WNT pathway is required to foster carcinogenesis. Cancer Prev Res; 9(6); 417-27.

Original languageEnglish (US)
Pages (from-to)417-427
Number of pages11
JournalCancer Prevention Research
Volume9
Issue number6
DOIs
StatePublished - Jun 1 2016

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Adenoma
Mucous Membrane
Carcinoma
Mutation
Carcinogenesis
Allelic Imbalance
Exome
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 5
Adenomatous Polyposis Coli
Chromosomes, Human, Pair 7
Systems Biology
Neoplasm Genes
Single Nucleotide Polymorphism
Colorectal Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Borras, E., Lucas, F. A. S., Chang, K., Zhou, R., Masand, G., Fowler, J., ... Vilar, E. (2016). Genomic landscape of colorectal mucosa and adenomas. Cancer Prevention Research, 9(6), 417-427. https://doi.org/10.1158/1940-6207.CAPR-16-0081

Genomic landscape of colorectal mucosa and adenomas. / Borras, Ester; Lucas, F. Anthony San; Chang, Kyle; Zhou, Ruoji; Masand, Gita; Fowler, Jerry; Mork, Maureen E.; You, Y. Nancy; Taggart, Melissa W.; McAllister, Florencia; Jones, David A.; Davies, Gareth E.; Edelmann, Winfried; Ehli, Erik A.; Lynch, Patrick M.; Hawk, Ernest T.; Capella, Gabriel; Scheet, Paul; Vilar, Eduardo.

In: Cancer Prevention Research, Vol. 9, No. 6, 01.06.2016, p. 417-427.

Research output: Contribution to journalArticle

Borras, E, Lucas, FAS, Chang, K, Zhou, R, Masand, G, Fowler, J, Mork, ME, You, YN, Taggart, MW, McAllister, F, Jones, DA, Davies, GE, Edelmann, W, Ehli, EA, Lynch, PM, Hawk, ET, Capella, G, Scheet, P & Vilar, E 2016, 'Genomic landscape of colorectal mucosa and adenomas', Cancer Prevention Research, vol. 9, no. 6, pp. 417-427. https://doi.org/10.1158/1940-6207.CAPR-16-0081
Borras E, Lucas FAS, Chang K, Zhou R, Masand G, Fowler J et al. Genomic landscape of colorectal mucosa and adenomas. Cancer Prevention Research. 2016 Jun 1;9(6):417-427. https://doi.org/10.1158/1940-6207.CAPR-16-0081
Borras, Ester ; Lucas, F. Anthony San ; Chang, Kyle ; Zhou, Ruoji ; Masand, Gita ; Fowler, Jerry ; Mork, Maureen E. ; You, Y. Nancy ; Taggart, Melissa W. ; McAllister, Florencia ; Jones, David A. ; Davies, Gareth E. ; Edelmann, Winfried ; Ehli, Erik A. ; Lynch, Patrick M. ; Hawk, Ernest T. ; Capella, Gabriel ; Scheet, Paul ; Vilar, Eduardo. / Genomic landscape of colorectal mucosa and adenomas. In: Cancer Prevention Research. 2016 ; Vol. 9, No. 6. pp. 417-427.
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