TY - JOUR
T1 - Genome scanning of higher eukaryotes by two-dimensional DNA typing using micro- and minisatellite core probes
AU - Uitterlinden, André G.
AU - Mullaart, Erik
AU - Morolli, Bruno
AU - Vijg, Jan
N1 - Funding Information:
This is publication number 5 of Medscand INGENY. The research described is supported by PBTS Grant BIO 89080 from the Dutch Ministry of Economic Affairs. We thank Drs. P. Lindhout and H. Sandbrink (Centre for Plant Breeding Research, Wageningen) for supplying tomato DNA, Dr. J. H. G. den Daas (Holland Genetics VOF, Arnhem) for supplying cattle blood, and Dr. R. Frants (Dept. of Anthropogenetics, State University Leiden) for supplying human DNA. We thank Dr. P. E. Slagboom (TNO-IVVO, Leiden) for helpful discussions. The 2-D DNA typing method is subject to patent property in the United States (patent
PY - 1991/10
Y1 - 1991/10
N2 - Electrophoretic separation of DNA restriction fragments in two dimensions according to two independent criteria, i.e., size and basepair sequence, allows all restriction fragments of DNA molecules of up to 5 million bp, such as the Escherichia coli genome, to be resolved in a single gel. For more complex genomes, hybridization analysis of the two-dimensional separation patterns is necessary to obtain interpretable data. Here we describe a detailed protocol for two-dimensional DNA typing of mammalian (human and cattle) and plant (tomato) genomic DNA using micro- and minisatellite core probes. Spot patterns comprising 100-800 spots were obtained. This method of simultaneously scoring hundreds of genetic loci has various applications e.g., in mapping genomes, defining DNA markers for hereditary traits by genetic linkage analysis, and detecting somatic instability.
AB - Electrophoretic separation of DNA restriction fragments in two dimensions according to two independent criteria, i.e., size and basepair sequence, allows all restriction fragments of DNA molecules of up to 5 million bp, such as the Escherichia coli genome, to be resolved in a single gel. For more complex genomes, hybridization analysis of the two-dimensional separation patterns is necessary to obtain interpretable data. Here we describe a detailed protocol for two-dimensional DNA typing of mammalian (human and cattle) and plant (tomato) genomic DNA using micro- and minisatellite core probes. Spot patterns comprising 100-800 spots were obtained. This method of simultaneously scoring hundreds of genetic loci has various applications e.g., in mapping genomes, defining DNA markers for hereditary traits by genetic linkage analysis, and detecting somatic instability.
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U2 - 10.1016/S1046-2023(05)80199-9
DO - 10.1016/S1046-2023(05)80199-9
M3 - Article
AN - SCOPUS:0344682171
SN - 1046-2023
VL - 3
SP - 83
EP - 90
JO - Methods
JF - Methods
IS - 2
ER -