Genetically separable functions of the MEC-17 tubulin acetyltransferase affect microtubule organization

Irini Topalidou, Charles Keller, Nereo Kalebic, Ken C.Q. Nguyen, Hannah Somhegyi, Kristin A. Politi, Paul Heppenstall, David H. Hall, Martin Chalfie

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Background: Microtubules (MTs) are formed from the lateral association of 11-16 protofilament chains of tubulin dimers, with most cells containing 13-protofilament (13-p) MTs. How these different MTs are formed is unknown, although the number of protofilaments may depend on the nature of the α- and β-tubulins. Results: Here we show that the enzymatic activity of the Caenorhabiditis elegans α-tubulin acetyltransferase (α-TAT) MEC-17 allows the production of 15-p MTs in the touch receptor neurons (TRNs) MTs. Without MEC-17, MTs with between 11 and 15 protofilaments are seen. Loss of this enzymatic activity also changes the number and organization of the TRN MTs and affects TRN axonal morphology. In contrast, enzymatically inactive MEC-17 is sufficient for touch sensitivity and proper process outgrowth without correcting the MT defects. Thus, in addition to demonstrating that MEC-17 is required for MT structure and organization, our results suggest that the large number of 15-p MTs, normally found in the TRNs, is not essential for mechanosensation. Conclusion: These experiments reveal a specific role for α-TAT in the formation of MTs and in the production of higher order MTs arrays. In addition, our results indicate that the α-TAT protein has functions that require acetyltransferase activity (such as the determination of protofilament number) and others that do not (presence of internal MT structures).

Original languageEnglish (US)
Pages (from-to)1057-1065
Number of pages9
JournalCurrent Biology
Volume22
Issue number12
DOIs
StatePublished - Jun 19 2012

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acetyltransferases
Acetyltransferases
Tubulin
tubulin
Microtubules
microtubules
Neurons
touch (sensation)
Touch
Dimers
neurons
receptors
Defects
Proteins
Experiments

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Genetically separable functions of the MEC-17 tubulin acetyltransferase affect microtubule organization. / Topalidou, Irini; Keller, Charles; Kalebic, Nereo; Nguyen, Ken C.Q.; Somhegyi, Hannah; Politi, Kristin A.; Heppenstall, Paul; Hall, David H.; Chalfie, Martin.

In: Current Biology, Vol. 22, No. 12, 19.06.2012, p. 1057-1065.

Research output: Contribution to journalArticle

Topalidou, I, Keller, C, Kalebic, N, Nguyen, KCQ, Somhegyi, H, Politi, KA, Heppenstall, P, Hall, DH & Chalfie, M 2012, 'Genetically separable functions of the MEC-17 tubulin acetyltransferase affect microtubule organization', Current Biology, vol. 22, no. 12, pp. 1057-1065. https://doi.org/10.1016/j.cub.2012.03.066
Topalidou, Irini ; Keller, Charles ; Kalebic, Nereo ; Nguyen, Ken C.Q. ; Somhegyi, Hannah ; Politi, Kristin A. ; Heppenstall, Paul ; Hall, David H. ; Chalfie, Martin. / Genetically separable functions of the MEC-17 tubulin acetyltransferase affect microtubule organization. In: Current Biology. 2012 ; Vol. 22, No. 12. pp. 1057-1065.
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