TY - JOUR
T1 - Gelation of sickle cell haemoglobin. II. Methaemoglobin
AU - Briehl, Robin W.
AU - Ewert, Sandra M.
PY - 1974/11/15
Y1 - 1974/11/15
N2 - Sickle cell methaemoglobin was assayed for gel formation by an equilibrium ultracentrifugation method previously described. A phase change from sol to gel, indicative of gelation, occurred, depending on conditions, at concentrations between 0.35 and 0.5 g/ml, considerably higher than concentrations observed previously for gelation of deoxyhaemoglobin S. Inositol hexaphosphate favours gelation, but gelation is seen also in its absence. Lowering pH toward 6 favours gelation. If gelation is assumed to require molecules in the T quaternary conformation, these results provide further evidence that methaemoglobin exists in R-T equilibrium in solution and that this equilibrium lies between the extremes exhibited by deoxyhaemoglobin (T-state) and carbon monoxide or oxyhaemoglobin (R-state).
AB - Sickle cell methaemoglobin was assayed for gel formation by an equilibrium ultracentrifugation method previously described. A phase change from sol to gel, indicative of gelation, occurred, depending on conditions, at concentrations between 0.35 and 0.5 g/ml, considerably higher than concentrations observed previously for gelation of deoxyhaemoglobin S. Inositol hexaphosphate favours gelation, but gelation is seen also in its absence. Lowering pH toward 6 favours gelation. If gelation is assumed to require molecules in the T quaternary conformation, these results provide further evidence that methaemoglobin exists in R-T equilibrium in solution and that this equilibrium lies between the extremes exhibited by deoxyhaemoglobin (T-state) and carbon monoxide or oxyhaemoglobin (R-state).
UR - http://www.scopus.com/inward/record.url?scp=0016312666&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016312666&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(74)90050-3
DO - 10.1016/0022-2836(74)90050-3
M3 - Article
C2 - 4449131
AN - SCOPUS:0016312666
SN - 0022-2836
VL - 89
SP - 759-762,IN51-IN52,763-766
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 4
ER -