Gap junctions mediate bystander cell death in developing retina

Karen Cusato, Alejandra Bosco, Renato Rozental, Cinthya A. Guimarães, Benjamin E. Reese, Rafael Linden, David C. Spray

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

During development of the retina, programmed cell death helps to establish the final size and distribution of various cell classes in distinct layers of the tissue. Here we show that dying cells in the developing ganglion and inner nuclear layers are clustered spatially and that gap junction inhibitors decrease the clustering of dying cells. To confirm the role of gap junctions in cell death, we induced targeted cell death via intracellular cytochrome c (Cc) and examined the induced cells and their neighbors for apoptotic morphology or caspase-3 cleavage. These studies indicate that bystander killing extends to coupled cells. Quantitative studies of bystander killing were performed by scrape-loading retinas with Cc in the presence of rhodamine dextran (RD; to identify Cc-loaded cells) and by counting pyknotic cells in cryosections. Although only 1.5% of control scrape-loaded cells (RD alone) showed apoptotic morphology, 97% of Cc scrape-loaded cells were pyknotic. Moreover, bystander killing extended to neighboring cells, not labeled with RD, and was reduced significantly by the gap junction inhibitors octanol and carbenoxolone. We hypothesize that dying cells in the retina generate a gap junction - permeant apoptotic signal that mediates bystander killing. This novel finding of naturally occurring bystander cell death may have important implications in the histogenesis and pathology of the nervous system.

Original languageEnglish (US)
Pages (from-to)6413-6422
Number of pages10
JournalJournal of Neuroscience
Volume23
Issue number16
StatePublished - Jul 23 2003

Fingerprint

Gap Junctions
Retina
Cell Death
Cytochromes c
Carbenoxolone
Octanols
Ganglia
Caspase 3
Nervous System
Cluster Analysis
Pathology

Keywords

  • Bystander effect
  • Cell death
  • Development
  • Gap junctions
  • Inner nuclear layer
  • Retina

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cusato, K., Bosco, A., Rozental, R., Guimarães, C. A., Reese, B. E., Linden, R., & Spray, D. C. (2003). Gap junctions mediate bystander cell death in developing retina. Journal of Neuroscience, 23(16), 6413-6422.

Gap junctions mediate bystander cell death in developing retina. / Cusato, Karen; Bosco, Alejandra; Rozental, Renato; Guimarães, Cinthya A.; Reese, Benjamin E.; Linden, Rafael; Spray, David C.

In: Journal of Neuroscience, Vol. 23, No. 16, 23.07.2003, p. 6413-6422.

Research output: Contribution to journalArticle

Cusato, K, Bosco, A, Rozental, R, Guimarães, CA, Reese, BE, Linden, R & Spray, DC 2003, 'Gap junctions mediate bystander cell death in developing retina', Journal of Neuroscience, vol. 23, no. 16, pp. 6413-6422.
Cusato K, Bosco A, Rozental R, Guimarães CA, Reese BE, Linden R et al. Gap junctions mediate bystander cell death in developing retina. Journal of Neuroscience. 2003 Jul 23;23(16):6413-6422.
Cusato, Karen ; Bosco, Alejandra ; Rozental, Renato ; Guimarães, Cinthya A. ; Reese, Benjamin E. ; Linden, Rafael ; Spray, David C. / Gap junctions mediate bystander cell death in developing retina. In: Journal of Neuroscience. 2003 ; Vol. 23, No. 16. pp. 6413-6422.
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