Gap junction distribution is altered between cardiac myocytes infected with Trypanosoma cruzi

A. C. Campos De Carvalho, H. B. Tanowitz, M. Wittner, R. Dermietzel, C. Roy, E. L. Hertzberg, David C. Spray

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Conduction disturbances frequently accompany both acute and chronic Chagas' disease. To explore the possibility that changes in gap junction distribution or abundance might play a role in these disturbances, we have investigated intercellular communication between rat neonatal cardiac myocytes in cultures infected with Trypanosoma cruzi. Contractile activity of infected cells was characterized by regional asynchrony within the culture as well as by irregular contraction patterns. Junctional conductance between infected cell pairs was found to be significantly lower than in uninfected cell pairs, and the rapidity and extent of intercellular transfer of the dye lucifer yellow was markedly reduced between infected cells. Immunocytochemical studies demonstrated that the parasitic infection significantly decreased connexin43 expression at junctional membrane regions, correlating with the detected functional uncoupling. These findings of reduced gap junction abundance and function in trypanosome-infected cells may provide important insight into the pathogenesis of the cardiac arrhythmias that attend Chagas' disease.

Original languageEnglish (US)
Pages (from-to)733-742
Number of pages10
JournalCirculation Research
Volume70
Issue number4
StatePublished - 1990

Fingerprint

Trypanosoma cruzi
Gap Junctions
Cardiac Myocytes
Chagas Disease
Connexin 43
Parasitic Diseases
Trypanosomiasis
Cardiac Arrhythmias
Chronic Disease
Coloring Agents
Membranes

Keywords

  • Chagas' disease
  • Chagasic cardiomyopathy
  • Connexin43
  • Heart
  • Parasite

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Campos De Carvalho, A. C., Tanowitz, H. B., Wittner, M., Dermietzel, R., Roy, C., Hertzberg, E. L., & Spray, D. C. (1990). Gap junction distribution is altered between cardiac myocytes infected with Trypanosoma cruzi. Circulation Research, 70(4), 733-742.

Gap junction distribution is altered between cardiac myocytes infected with Trypanosoma cruzi. / Campos De Carvalho, A. C.; Tanowitz, H. B.; Wittner, M.; Dermietzel, R.; Roy, C.; Hertzberg, E. L.; Spray, David C.

In: Circulation Research, Vol. 70, No. 4, 1990, p. 733-742.

Research output: Contribution to journalArticle

Campos De Carvalho, AC, Tanowitz, HB, Wittner, M, Dermietzel, R, Roy, C, Hertzberg, EL & Spray, DC 1990, 'Gap junction distribution is altered between cardiac myocytes infected with Trypanosoma cruzi', Circulation Research, vol. 70, no. 4, pp. 733-742.
Campos De Carvalho AC, Tanowitz HB, Wittner M, Dermietzel R, Roy C, Hertzberg EL et al. Gap junction distribution is altered between cardiac myocytes infected with Trypanosoma cruzi. Circulation Research. 1990;70(4):733-742.
Campos De Carvalho, A. C. ; Tanowitz, H. B. ; Wittner, M. ; Dermietzel, R. ; Roy, C. ; Hertzberg, E. L. ; Spray, David C. / Gap junction distribution is altered between cardiac myocytes infected with Trypanosoma cruzi. In: Circulation Research. 1990 ; Vol. 70, No. 4. pp. 733-742.
@article{3271c2f3a8c14d30a8089e54ecdaa0d3,
title = "Gap junction distribution is altered between cardiac myocytes infected with Trypanosoma cruzi",
abstract = "Conduction disturbances frequently accompany both acute and chronic Chagas' disease. To explore the possibility that changes in gap junction distribution or abundance might play a role in these disturbances, we have investigated intercellular communication between rat neonatal cardiac myocytes in cultures infected with Trypanosoma cruzi. Contractile activity of infected cells was characterized by regional asynchrony within the culture as well as by irregular contraction patterns. Junctional conductance between infected cell pairs was found to be significantly lower than in uninfected cell pairs, and the rapidity and extent of intercellular transfer of the dye lucifer yellow was markedly reduced between infected cells. Immunocytochemical studies demonstrated that the parasitic infection significantly decreased connexin43 expression at junctional membrane regions, correlating with the detected functional uncoupling. These findings of reduced gap junction abundance and function in trypanosome-infected cells may provide important insight into the pathogenesis of the cardiac arrhythmias that attend Chagas' disease.",
keywords = "Chagas' disease, Chagasic cardiomyopathy, Connexin43, Heart, Parasite",
author = "{Campos De Carvalho}, {A. C.} and Tanowitz, {H. B.} and M. Wittner and R. Dermietzel and C. Roy and Hertzberg, {E. L.} and Spray, {David C.}",
year = "1990",
language = "English (US)",
volume = "70",
pages = "733--742",
journal = "Circulation Research",
issn = "0009-7330",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Gap junction distribution is altered between cardiac myocytes infected with Trypanosoma cruzi

AU - Campos De Carvalho, A. C.

AU - Tanowitz, H. B.

AU - Wittner, M.

AU - Dermietzel, R.

AU - Roy, C.

AU - Hertzberg, E. L.

AU - Spray, David C.

PY - 1990

Y1 - 1990

N2 - Conduction disturbances frequently accompany both acute and chronic Chagas' disease. To explore the possibility that changes in gap junction distribution or abundance might play a role in these disturbances, we have investigated intercellular communication between rat neonatal cardiac myocytes in cultures infected with Trypanosoma cruzi. Contractile activity of infected cells was characterized by regional asynchrony within the culture as well as by irregular contraction patterns. Junctional conductance between infected cell pairs was found to be significantly lower than in uninfected cell pairs, and the rapidity and extent of intercellular transfer of the dye lucifer yellow was markedly reduced between infected cells. Immunocytochemical studies demonstrated that the parasitic infection significantly decreased connexin43 expression at junctional membrane regions, correlating with the detected functional uncoupling. These findings of reduced gap junction abundance and function in trypanosome-infected cells may provide important insight into the pathogenesis of the cardiac arrhythmias that attend Chagas' disease.

AB - Conduction disturbances frequently accompany both acute and chronic Chagas' disease. To explore the possibility that changes in gap junction distribution or abundance might play a role in these disturbances, we have investigated intercellular communication between rat neonatal cardiac myocytes in cultures infected with Trypanosoma cruzi. Contractile activity of infected cells was characterized by regional asynchrony within the culture as well as by irregular contraction patterns. Junctional conductance between infected cell pairs was found to be significantly lower than in uninfected cell pairs, and the rapidity and extent of intercellular transfer of the dye lucifer yellow was markedly reduced between infected cells. Immunocytochemical studies demonstrated that the parasitic infection significantly decreased connexin43 expression at junctional membrane regions, correlating with the detected functional uncoupling. These findings of reduced gap junction abundance and function in trypanosome-infected cells may provide important insight into the pathogenesis of the cardiac arrhythmias that attend Chagas' disease.

KW - Chagas' disease

KW - Chagasic cardiomyopathy

KW - Connexin43

KW - Heart

KW - Parasite

UR - http://www.scopus.com/inward/record.url?scp=0026583587&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026583587&partnerID=8YFLogxK

M3 - Article

C2 - 1551199

AN - SCOPUS:0026583587

VL - 70

SP - 733

EP - 742

JO - Circulation Research

JF - Circulation Research

SN - 0009-7330

IS - 4

ER -