Functions of the stem region of the Semliki Forest virus fusion protein during virus fusion and assembly

Maofu Liao, Margaret Kielian

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Membrane fusion of the alphaviruses is mediated by the E1 protein, a class II virus membrane fusion protein. During fusion, E1 dissociates from its heterodimer interaction with the E2 protein and forms a target membrane-inserted E1 homotrimer. The structure of the homotrimer is that of a trimeric hairpin in which E1 domain III and the stem region fold back toward the target membrane-inserted fusion peptide loop. The E1 stem region has a strictly conserved length and several highly conserved residues, suggesting the possibility of specific stem interactions along the trimer core and an important role in driving membrane fusion. Mutagenesis studies of the alphavirus Semliki Forest virus (SFV) here demonstrated that there was a strong requirement for the E1 stem in virus assembly and budding, probably reflecting its importance in lateral interactions of the envelope proteins. Surprisingly, however, neither the conserved length nor any specific residues of the stem were required for membrane fusion. Although the highest fusion activity was observed with wild-type E1, efficient fusion was mediated by stem mutants containing a variety of substitutions or deletions. A minimal stem length was required but could be conferred by a series of alanine residues. The lack of a specific stem sequence requirement during SFV fusion suggests that the interaction of domain III with the trimer core can provide sufficient driving force to mediate membrane merger.

Original languageEnglish (US)
Pages (from-to)11362-11369
Number of pages8
JournalJournal of Virology
Volume80
Issue number22
DOIs
StatePublished - Nov 2006

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viral fusion proteins
Semliki Forest virus
Viral Fusion Proteins
Semliki forest virus
Virus Assembly
Membrane Fusion
Alphavirus
viruses
stems
Membrane Fusion Proteins
Virus Release
Virus Internalization
Proteins
Membranes
Mutagenesis
Alanine
proteins
Peptides
mutagenesis
alanine

ASJC Scopus subject areas

  • Immunology

Cite this

Functions of the stem region of the Semliki Forest virus fusion protein during virus fusion and assembly. / Liao, Maofu; Kielian, Margaret.

In: Journal of Virology, Vol. 80, No. 22, 11.2006, p. 11362-11369.

Research output: Contribution to journalArticle

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