Functional characterization of a putative aquaporin from Encephalitozoon cuniculi, a microsporidia pathogenic to humans

Kaya Ghosh, Clint D. Cappiello, Sean M. McBride, James L. Occi, Ann Cali, Peter M. Takvorian, Thomas V. McDonald, Louis M. Weiss

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The microsporidia are a group of obligate intracellular parasitic protists that have been implicated as both human and veterinary pathogens. The infectious process of these organisms is believed to be dependent upon the rapid influx of water into spores, presumably via aquaporins (AQPs), transmembrane channels that facilitate osmosis. An AQP-like sequence of the microsporidium Encephalitozoon cuniculi (EcAQP), when cloned and expressed in oocytes of Xenopus laevis, rendered these oocytes highly permeable to water. No permeability to the solutes glycerol or urea was observed. Pre-treatment of EcAQP-expressing oocytes with HgCl2 failed to inhibit their osmotic permeability, as predicted from EcAQP's lack of mercury-sensitive cysteine residues near the NPA motifs which line the AQP aqueous pore. EcAQP exhibits sequence identity to AQP A of Dictyostelium discoideum (26%) and human AQP 2 (24%). Further study of AQPs in microsporidia and their potential inhibitors may yield novel therapeutic agents for microsporidian infections.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalInternational Journal for Parasitology
Volume36
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Encephalitozoon cuniculi
Microsporidia
Aquaporins
Oocytes
Permeability
Unclassified Microsporidia
Aquaporin 2
Osmosis
Mercuric Chloride
Dictyostelium
Water
Xenopus laevis
Spores
Mercury
Glycerol
Cysteine
Urea
Infection

Keywords

  • Aquaporin
  • Encephalitozoon
  • Germination
  • Microsporidia
  • Water permeability
  • Xenopus oocytes

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Functional characterization of a putative aquaporin from Encephalitozoon cuniculi, a microsporidia pathogenic to humans. / Ghosh, Kaya; Cappiello, Clint D.; McBride, Sean M.; Occi, James L.; Cali, Ann; Takvorian, Peter M.; McDonald, Thomas V.; Weiss, Louis M.

In: International Journal for Parasitology, Vol. 36, No. 1, 01.2006, p. 57-62.

Research output: Contribution to journalArticle

Ghosh, Kaya ; Cappiello, Clint D. ; McBride, Sean M. ; Occi, James L. ; Cali, Ann ; Takvorian, Peter M. ; McDonald, Thomas V. ; Weiss, Louis M. / Functional characterization of a putative aquaporin from Encephalitozoon cuniculi, a microsporidia pathogenic to humans. In: International Journal for Parasitology. 2006 ; Vol. 36, No. 1. pp. 57-62.
@article{835a6b3dea2a467391bb4638f3c45994,
title = "Functional characterization of a putative aquaporin from Encephalitozoon cuniculi, a microsporidia pathogenic to humans",
abstract = "The microsporidia are a group of obligate intracellular parasitic protists that have been implicated as both human and veterinary pathogens. The infectious process of these organisms is believed to be dependent upon the rapid influx of water into spores, presumably via aquaporins (AQPs), transmembrane channels that facilitate osmosis. An AQP-like sequence of the microsporidium Encephalitozoon cuniculi (EcAQP), when cloned and expressed in oocytes of Xenopus laevis, rendered these oocytes highly permeable to water. No permeability to the solutes glycerol or urea was observed. Pre-treatment of EcAQP-expressing oocytes with HgCl2 failed to inhibit their osmotic permeability, as predicted from EcAQP's lack of mercury-sensitive cysteine residues near the NPA motifs which line the AQP aqueous pore. EcAQP exhibits sequence identity to AQP A of Dictyostelium discoideum (26{\%}) and human AQP 2 (24{\%}). Further study of AQPs in microsporidia and their potential inhibitors may yield novel therapeutic agents for microsporidian infections.",
keywords = "Aquaporin, Encephalitozoon, Germination, Microsporidia, Water permeability, Xenopus oocytes",
author = "Kaya Ghosh and Cappiello, {Clint D.} and McBride, {Sean M.} and Occi, {James L.} and Ann Cali and Takvorian, {Peter M.} and McDonald, {Thomas V.} and Weiss, {Louis M.}",
year = "2006",
month = "1",
doi = "10.1016/j.ijpara.2005.08.013",
language = "English (US)",
volume = "36",
pages = "57--62",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Functional characterization of a putative aquaporin from Encephalitozoon cuniculi, a microsporidia pathogenic to humans

AU - Ghosh, Kaya

AU - Cappiello, Clint D.

AU - McBride, Sean M.

AU - Occi, James L.

AU - Cali, Ann

AU - Takvorian, Peter M.

AU - McDonald, Thomas V.

AU - Weiss, Louis M.

PY - 2006/1

Y1 - 2006/1

N2 - The microsporidia are a group of obligate intracellular parasitic protists that have been implicated as both human and veterinary pathogens. The infectious process of these organisms is believed to be dependent upon the rapid influx of water into spores, presumably via aquaporins (AQPs), transmembrane channels that facilitate osmosis. An AQP-like sequence of the microsporidium Encephalitozoon cuniculi (EcAQP), when cloned and expressed in oocytes of Xenopus laevis, rendered these oocytes highly permeable to water. No permeability to the solutes glycerol or urea was observed. Pre-treatment of EcAQP-expressing oocytes with HgCl2 failed to inhibit their osmotic permeability, as predicted from EcAQP's lack of mercury-sensitive cysteine residues near the NPA motifs which line the AQP aqueous pore. EcAQP exhibits sequence identity to AQP A of Dictyostelium discoideum (26%) and human AQP 2 (24%). Further study of AQPs in microsporidia and their potential inhibitors may yield novel therapeutic agents for microsporidian infections.

AB - The microsporidia are a group of obligate intracellular parasitic protists that have been implicated as both human and veterinary pathogens. The infectious process of these organisms is believed to be dependent upon the rapid influx of water into spores, presumably via aquaporins (AQPs), transmembrane channels that facilitate osmosis. An AQP-like sequence of the microsporidium Encephalitozoon cuniculi (EcAQP), when cloned and expressed in oocytes of Xenopus laevis, rendered these oocytes highly permeable to water. No permeability to the solutes glycerol or urea was observed. Pre-treatment of EcAQP-expressing oocytes with HgCl2 failed to inhibit their osmotic permeability, as predicted from EcAQP's lack of mercury-sensitive cysteine residues near the NPA motifs which line the AQP aqueous pore. EcAQP exhibits sequence identity to AQP A of Dictyostelium discoideum (26%) and human AQP 2 (24%). Further study of AQPs in microsporidia and their potential inhibitors may yield novel therapeutic agents for microsporidian infections.

KW - Aquaporin

KW - Encephalitozoon

KW - Germination

KW - Microsporidia

KW - Water permeability

KW - Xenopus oocytes

UR - http://www.scopus.com/inward/record.url?scp=30044439993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30044439993&partnerID=8YFLogxK

U2 - 10.1016/j.ijpara.2005.08.013

DO - 10.1016/j.ijpara.2005.08.013

M3 - Article

VL - 36

SP - 57

EP - 62

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 1

ER -