Function and structural organization of Mot1 bound to a natural target promoter

Rebekka O. Sprouse, Inna Shcherbakova, Huiyong Cheng, Elizabeth Jamison, Michael D. Brenowitz, David T. Auble

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Mot1 is an essential, conserved TATA-binding protein (TBP)-associated factor in Saccharomyces cerevisiae and a member of the Snf2/Swi2 ATPase family. Mot1 uses ATP hydrolysis to displace TBP from DNA, an activity that can be readily reconciled with its global role in gene repression. Less well understood is how Mot1 directly activates gene expression. It has been suggested that Mot1-mediated activation can occur by displacement of inactive TBP-containing complexes from promoters, thereby permitting assembly of functional transcription complexes. Mot1 may also activate transcription by other mechanisms that have not yet been defined. A gap in our understanding has been the absence of biochemical information related to the activity of Mot1 on natural target genes. Using URA1 as a model Mot1-activated promoter, we show striking differences in the way that both TBP and Mot1 interact with DNA compared with other model DNA substrates analyzed previously. These differences are due at least in part to the propensity of TBP alone to bind to the URA1 promoter in the wrong orientation to direct appropriate assembly of the URA1 preinitiation complex. The results suggest that Mot1-mediated activation of URA1 transcription involves at least two steps, one of which is the removal of TBP bound to the promoter in the opposite orientation required for URA1 transcription.

Original languageEnglish (US)
Pages (from-to)24935-24948
Number of pages14
JournalJournal of Biological Chemistry
Volume283
Issue number36
DOIs
StatePublished - Sep 5 2008

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TATA-Box Binding Protein
Transcription
DNA
TATA-Binding Protein Associated Factors
Genes
Chemical activation
Gene expression
Yeast
Transcriptional Activation
Saccharomyces cerevisiae
Adenosine Triphosphatases
Hydrolysis
Adenosine Triphosphate
Gene Expression
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Function and structural organization of Mot1 bound to a natural target promoter. / Sprouse, Rebekka O.; Shcherbakova, Inna; Cheng, Huiyong; Jamison, Elizabeth; Brenowitz, Michael D.; Auble, David T.

In: Journal of Biological Chemistry, Vol. 283, No. 36, 05.09.2008, p. 24935-24948.

Research output: Contribution to journalArticle

Sprouse, RO, Shcherbakova, I, Cheng, H, Jamison, E, Brenowitz, MD & Auble, DT 2008, 'Function and structural organization of Mot1 bound to a natural target promoter', Journal of Biological Chemistry, vol. 283, no. 36, pp. 24935-24948. https://doi.org/10.1074/jbc.M803749200
Sprouse, Rebekka O. ; Shcherbakova, Inna ; Cheng, Huiyong ; Jamison, Elizabeth ; Brenowitz, Michael D. ; Auble, David T. / Function and structural organization of Mot1 bound to a natural target promoter. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 36. pp. 24935-24948.
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