Formation of methotrexate polyglutamates in rat hepatocytes

D. A. Gewirtz, J. C. White, J. K. Randolph, I. David Goldman

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Polyglutamate derivatives of [3H]methotrexate (MTX) were detected in freshly isolated rat hepatocytes in suspension within 15 min after exposure to the folate analog. The rate of polyglutamate synthesis remained constant for at least one hr, and the polyglutamate derivatives accounted for an increasing proportion of the intracellular radiolabel with time. After initial exposure to 1 μM [3H]MTX, polyglutamate derivatives of MTX continued to be synthesized even after the extracellular [3H]MTX concentration had been reduced 20-fold. Prolonged exposure of hepatocytes in primary culture to 1 μM [3H]MTX resulted in the formation of longer-chain polyglutamate derivatives of MTX. The present studies demonstrate another important biosynthetic capacity of the freshly isolated hepatocyte and suggest the usefulness of this system for studying the mechanism of, and controlling factors in, the synthesis of polyglutamate derivatives of MTX. The ramifications of the formation of MTX polyglutamates on drug cytotoxicity in general and hepatotoxicity in particular are considered.

Original languageEnglish (US)
Pages (from-to)2914-2918
Number of pages5
JournalCancer Research
Volume39
Issue number8
StatePublished - 1979
Externally publishedYes

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Polyglutamic Acid
Methotrexate
Hepatocytes
Folic Acid
Suspensions
methotrexate polyglutamate
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Gewirtz, D. A., White, J. C., Randolph, J. K., & Goldman, I. D. (1979). Formation of methotrexate polyglutamates in rat hepatocytes. Cancer Research, 39(8), 2914-2918.

Formation of methotrexate polyglutamates in rat hepatocytes. / Gewirtz, D. A.; White, J. C.; Randolph, J. K.; Goldman, I. David.

In: Cancer Research, Vol. 39, No. 8, 1979, p. 2914-2918.

Research output: Contribution to journalArticle

Gewirtz, DA, White, JC, Randolph, JK & Goldman, ID 1979, 'Formation of methotrexate polyglutamates in rat hepatocytes', Cancer Research, vol. 39, no. 8, pp. 2914-2918.
Gewirtz DA, White JC, Randolph JK, Goldman ID. Formation of methotrexate polyglutamates in rat hepatocytes. Cancer Research. 1979;39(8):2914-2918.
Gewirtz, D. A. ; White, J. C. ; Randolph, J. K. ; Goldman, I. David. / Formation of methotrexate polyglutamates in rat hepatocytes. In: Cancer Research. 1979 ; Vol. 39, No. 8. pp. 2914-2918.
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